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Isolation and characterization of a new Helicobacter sp. belonging to the 'Helicobacter heilmannii' type 2 group from feline stomachs

Annemieke Smet UGent, Frank Pasmans UGent, Bram Flahou UGent, Katharina D'Herde UGent, Peter Vandamme UGent, Ilse Cleenwerck UGent, Richard Ducatelle UGent and Freddy Haesebrouck UGent (2010) 9th international workshop on pathogenesis and host response in helicobacter infections, Abstracts.
abstract
Three Gram-negative, microaerophillic bacteria with a corkscrew-like morphology isolated from the gastric mucosa of cats and designated ASB1, ABS2 and ASB3, were subjected to a polyphasic taxonomic study. The isolates grew on biphasic culture plates in microaerobic conditions at 37°C and exhibited urease, oxidase and catalase activity. They were also able to grow in colonies on dry agar plates. Based on 16S rRNA gene sequence analysis, ASB1, ABS2 and ASB3 were identified as a member of the genus Helicobacter and showed 98 to 99% sequence similarity to H. felis, H. bizzozeronii, Candidatus H. heilmannii, H. cynogastricus and H. salomonis, five related Helicobacter species belonging to the “Helicobacter heilmannii” type 2 group and previously detected in the feline or canine gastric mucosa. Sequencing of the partial hsp60 gene demostrated that ASB1, ASB2 and ASB3 constitute a separate taxon in the “Helicobacter heilmannii” type 2 group. The urease gene sequences of ASB1, ASB2 and ASB3 showed approximately 92% homology to the urease gene sequences of “Candidatus Helicobacter heilmannii” described by O’Rourke et al. (2004). Protein profiling of the strains ASB1, ASB2 and ASB3 using SDS-PAGE also allowed to differentiate them from other Helicobacter species of feline or canine gastric origin. The results of this polyphasic taxonomic study indicate that the cultured isolates constitute a new taxon corresponding to “Candidatus Helicobacter heilmannii” from cats but further investigations are still needed.
Please use this url to cite or link to this publication:
author
organization
year
type
conference
publication status
published
subject
in
9th international workshop on pathogenesis and host response in helicobacter infections, Abstracts
conference name
9th International Workshop on Pathogenesis and Host Response in Helicobacter Infections
conference location
Helsingor, Denmark
conference start
2010-07-07
conference end
2010-07-10
language
English
UGent publication?
yes
classification
C3
copyright statement
I have retained and own the full copyright for this publication
id
1217498
handle
http://hdl.handle.net/1854/LU-1217498
date created
2011-05-05 11:33:24
date last changed
2011-05-06 11:07:13
@inproceedings{1217498,
  abstract     = {Three Gram-negative, microaerophillic bacteria with a corkscrew-like morphology isolated from the gastric mucosa of cats and designated ASB1, ABS2 and ASB3, were subjected to a polyphasic taxonomic study. The isolates grew on biphasic culture plates in microaerobic conditions at 37{\textdegree}C and exhibited urease, oxidase and catalase activity. They were also able to grow in colonies on dry agar plates. Based on 16S rRNA gene sequence analysis, ASB1, ABS2 and ASB3 were identified as a member of the genus Helicobacter and showed 98 to 99\% sequence similarity to H. felis, H. bizzozeronii, Candidatus H. heilmannii, H. cynogastricus and H. salomonis, five related Helicobacter species belonging to the {\textquotedblleft}Helicobacter heilmannii{\textquotedblright} type 2 group and previously detected in the feline or canine gastric mucosa. Sequencing of the partial hsp60 gene demostrated that ASB1, ASB2 and ASB3 constitute a separate taxon in the {\textquotedblleft}Helicobacter heilmannii{\textquotedblright} type 2 group. The urease gene sequences of ASB1, ASB2 and ASB3 showed approximately 92\% homology to the urease gene sequences of {\textquotedblleft}Candidatus Helicobacter heilmannii{\textquotedblright} described by O{\textquoteright}Rourke et al. (2004). Protein profiling of the strains ASB1, ASB2 and ASB3 using SDS-PAGE also allowed to differentiate them from other Helicobacter species of feline or canine gastric origin. The results of this polyphasic taxonomic study indicate that the cultured isolates constitute a new taxon corresponding to {\textquotedblleft}Candidatus Helicobacter heilmannii{\textquotedblright} from cats but further investigations are still needed.},
  author       = {Smet, Annemieke and Pasmans, Frank and Flahou, Bram and D'Herde, Katharina and Vandamme, Peter and Cleenwerck, Ilse and Ducatelle, Richard and Haesebrouck, Freddy},
  booktitle    = {9th international workshop on pathogenesis and host response in helicobacter infections, Abstracts},
  language     = {eng},
  location     = {Helsingor, Denmark},
  title        = {Isolation and characterization of a new Helicobacter sp. belonging to the 'Helicobacter heilmannii' type 2 group from feline stomachs},
  year         = {2010},
}

Chicago
Smet, Annemieke, Frank Pasmans, Bram Flahou, Katharina D’Herde, Peter Vandamme, Ilse Cleenwerck, Richard Ducatelle, and Freddy Haesebrouck. 2010. “Isolation and Characterization of a New Helicobacter Sp. Belonging to the ‘Helicobacter Heilmannii’ Type 2 Group from Feline Stomachs.” In 9th International Workshop on Pathogenesis and Host Response in Helicobacter Infections, Abstracts.
APA
Smet, A., Pasmans, F., Flahou, B., D’Herde, K., Vandamme, P., Cleenwerck, I., Ducatelle, R., et al. (2010). Isolation and characterization of a new Helicobacter sp. belonging to the “Helicobacter heilmannii” type 2 group from feline stomachs. 9th international workshop on pathogenesis and host response in helicobacter infections, Abstracts. Presented at the 9th International Workshop on Pathogenesis and Host Response in Helicobacter Infections.
Vancouver
1.
Smet A, Pasmans F, Flahou B, D’Herde K, Vandamme P, Cleenwerck I, et al. Isolation and characterization of a new Helicobacter sp. belonging to the “Helicobacter heilmannii” type 2 group from feline stomachs. 9th international workshop on pathogenesis and host response in helicobacter infections, Abstracts. 2010.
MLA
Smet, Annemieke, Frank Pasmans, Bram Flahou, et al. “Isolation and Characterization of a New Helicobacter Sp. Belonging to the ‘Helicobacter Heilmannii’ Type 2 Group from Feline Stomachs.” 9th International Workshop on Pathogenesis and Host Response in Helicobacter Infections, Abstracts. 2010. Print.