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In situ ESBL conjugation from avian to human Escherichia coli during cefotaxime administration

Annemieke Smet UGent, Geertrui Rasschaert, An Martel UGent, Davy Persoons UGent, Jeroen Dewulf UGent, Patrick Butaye UGent, Boudewijn Catry, Freddy Haesebrouck UGent, Lieve Herman and Marc Heyndrickx (2011) JOURNAL OF APPLIED MICROBIOLOGY. 110(2). p.541-549
abstract
Aims: The behaviour of an E. coli isolate of broiler origin harbouring a blaTEM-52-carrying plasmid (lactose negative mutant of B1-54, IncII group) was studied in an in situ continuous flow culture system, simulating the human caecum and the ascending colon during cefotaxime administration. Methods and Results: Fresh faeces from a healthy volunteer, negative for cephalosporin-resistant E. coli, were selected to prepare inocula. The microbiota was monitored by plating on diverse selective media, and a shift in the populations of bacteria was examined by 16S rDNA PCR denaturing gradient gel electrophoresis. E. coli transconjugants were verified by plasmid and pulsed field gel electrophoresis profiles (PFGE). The avian extended-spectrum β-lactamase (ESBL) positive E. coli was able to proliferate without selective pressure of cefotaxime and E. coli transconjugants of human origin were detected 24 h after inoculation of the donor strain. Upon administration of cefotaxime to the fresh medium, an increase of the population size of E. coli B1-54 and the transconjugants was observed. PFGE and plasmid analysis revealed a limited number of human E. coli clones receptive for the blaTEM-52-carrying plasmid. Conclusions: These observations provide evidence of the maintenance of an E. coli strain of poultry origin and the horizontal gene transfer in the human commensal bowel microbiota even without antimicrobial treatment. Significance and Impact of Study: The fact that an E. coli strain of poultry origin might establish itself and transfer its bla gene to commensal human E. coli raises public health concerns.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
conjugation, SALMONELLA, extended-spectrum beta-lactams, transfer frequency, SPECTRUM BETA-LACTAMASES, ANTIMICROBIAL RESISTANCE, AMPICILLIN RESISTANCE, ANTIBIOTIC-RESISTANCE, KLEBSIELLA-PNEUMONIAE, DIGESTIVE-TRACT, GENE-TRANSFER, VIVO TRANSFER, FARM-ANIMALS, continuous flow model
journal title
JOURNAL OF APPLIED MICROBIOLOGY
J. Appl. Microbiol.
volume
110
issue
2
pages
541 - 549
Web of Science type
Article
Web of Science id
000285972900017
JCR category
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
JCR impact factor
2.337 (2011)
JCR rank
71/157 (2011)
JCR quartile
2 (2011)
ISSN
1364-5072
DOI
10.1111/j.1365-2672.2010.04907.x
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1217468
handle
http://hdl.handle.net/1854/LU-1217468
date created
2011-05-05 11:24:47
date last changed
2013-03-01 00:30:32
@article{1217468,
  abstract     = {Aims: The behaviour of an E. coli isolate of broiler origin harbouring a blaTEM-52-carrying plasmid (lactose negative mutant of B1-54, IncII group) was studied in an in situ continuous flow culture system, simulating the human caecum and the ascending colon during cefotaxime administration. 
Methods and Results: Fresh faeces from a healthy volunteer, negative for cephalosporin-resistant E. coli, were selected to prepare inocula. The microbiota was monitored by plating on diverse selective media, and a shift in the populations of bacteria was examined by 16S rDNA PCR denaturing gradient gel electrophoresis. E. coli transconjugants were verified by plasmid and pulsed field gel electrophoresis profiles (PFGE). The avian extended-spectrum \ensuremath{\beta}-lactamase (ESBL) positive E. coli was able to proliferate without selective pressure of cefotaxime and E. coli transconjugants of human origin were detected 24 h after inoculation of the donor strain. Upon administration of cefotaxime to the fresh medium, an increase of the population size of E. coli B1-54 and the transconjugants was observed. PFGE and plasmid analysis revealed a limited number of human E. coli clones receptive for the blaTEM-52-carrying plasmid.
Conclusions: These observations provide evidence of the maintenance of an E. coli strain of poultry origin and the horizontal gene transfer in the human commensal bowel microbiota even without antimicrobial treatment.
Significance and Impact of Study: The fact that an E. coli strain of poultry origin might establish itself and transfer its bla gene to commensal human E. coli raises public health concerns.},
  author       = {Smet, Annemieke and Rasschaert, Geertrui and Martel, An and Persoons, Davy and Dewulf, Jeroen and Butaye, Patrick and Catry, Boudewijn and Haesebrouck, Freddy and Herman, Lieve and Heyndrickx, Marc},
  issn         = {1364-5072},
  journal      = {JOURNAL OF APPLIED MICROBIOLOGY},
  keyword      = {conjugation,SALMONELLA,extended-spectrum beta-lactams,transfer frequency,SPECTRUM BETA-LACTAMASES,ANTIMICROBIAL RESISTANCE,AMPICILLIN RESISTANCE,ANTIBIOTIC-RESISTANCE,KLEBSIELLA-PNEUMONIAE,DIGESTIVE-TRACT,GENE-TRANSFER,VIVO TRANSFER,FARM-ANIMALS,continuous flow model},
  language     = {eng},
  number       = {2},
  pages        = {541--549},
  title        = {In situ ESBL conjugation from avian to human Escherichia coli during cefotaxime administration},
  url          = {http://dx.doi.org/10.1111/j.1365-2672.2010.04907.x},
  volume       = {110},
  year         = {2011},
}

Chicago
Smet, Annemieke, Geertrui Rasschaert, An Martel, Davy Persoons, Jeroen Dewulf, Patrick Butaye, Boudewijn Catry, Freddy Haesebrouck, Lieve Herman, and Marc Heyndrickx. 2011. “In Situ ESBL Conjugation from Avian to Human Escherichia Coli During Cefotaxime Administration.” Journal of Applied Microbiology 110 (2): 541–549.
APA
Smet, A., Rasschaert, G., Martel, A., Persoons, D., Dewulf, J., Butaye, P., Catry, B., et al. (2011). In situ ESBL conjugation from avian to human Escherichia coli during cefotaxime administration. JOURNAL OF APPLIED MICROBIOLOGY, 110(2), 541–549.
Vancouver
1.
Smet A, Rasschaert G, Martel A, Persoons D, Dewulf J, Butaye P, et al. In situ ESBL conjugation from avian to human Escherichia coli during cefotaxime administration. JOURNAL OF APPLIED MICROBIOLOGY. 2011;110(2):541–9.
MLA
Smet, Annemieke, Geertrui Rasschaert, An Martel, et al. “In Situ ESBL Conjugation from Avian to Human Escherichia Coli During Cefotaxime Administration.” JOURNAL OF APPLIED MICROBIOLOGY 110.2 (2011): 541–549. Print.