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Helicobacter heilmannii sp. nov., isolated from feline gastric mucosa

Annemieke Smet UGent, Bram Flahou UGent, Katharina D'Herde UGent, Peter Vandamme UGent, Ilse Cleenwerck UGent, Richard Ducatelle UGent, Frank Pasmans UGent and Freddy Haesebrouck UGent (2012) INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY. 62(2). p.299-306
abstract
Three Gram-negative, microaerophilic bacteria, strains ASB1(T), ASB2 and ASB3, with a corkscrew-like morphology isolated from the gastric mucosa of cats were studied using a polyphasic taxonomic approach. The isolates grew on biphasic culture plates under microaerobic conditions at 37 degrees C and exhibited urease, oxidase and catalase activities. They were also able to grow in colonies on dry agar plates. Based on 16S rRNA gene sequence analysis, ASB1(T), ASB2 and ASB3 were identified as members of the genus Helicobacter and showed 98 to 99% sequence similarity to strains of Helicobacter felis, Helicobacter bizzozeronii, 'Candidatus Helicobacter heilmannii', Helicobacter cynogastricus, Helicobacter baculiformis and Helicobacter salomonis, six related Helicobacter species previously detected in feline or canine gastric mucosa. Sequencing of the partial hsp60 gene demonstrated that ASB1(T), ASB2 and ASB3 constitute a separate taxon among the feline and canine Helicobacter species. The urease gene sequences of ASB1(T), ASB2 and ASB3 showed approximately 91% similarity to those of 'Candidatus Helicobacter heilmannii'. Protein profiling, the absence of alkaline phosphatase activity and several other biochemical characteristics also allowed strains ASB1(T), ASB2 and ASB3 to be differentiated from other Helicobacter species of feline or canine gastric origin. The results of this polyphasic taxonomic study show that the cultured isolates constitute a new taxon corresponding to 'Candidatus Helicobacter heilmannii', which was previously demonstrated in the stomach of humans, wild felidae, cats and dogs. The name Helicobacter heilmannii sp. nov. is proposed for these isolates; the type strain is ASB1(T) (=DSM 23983(T)=LMG 26292(T)).
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
CATS, PREVALENCE, ORGANISMS, BIZZOZERONII, IDENTIFICATION, DOMESTIC-ANIMALS, CANINE, SAMPLES, INFECTION, STOMACH
journal title
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
Int. J. Syst. Evol. Microbiol.
volume
62
issue
2
pages
299 - 306
Web of Science type
Article
Web of Science id
000300861600007
JCR category
MICROBIOLOGY
JCR impact factor
2.112 (2012)
JCR rank
68/116 (2012)
JCR quartile
3 (2012)
ISSN
1466-5026
DOI
10.1099/ijs.0.029207-0
language
English
UGent publication?
yes
classification
A1
additional info
correction published in Int. J. Syst. Evol. Microbiol. (2012) 62(4), 1016 ; DOI 10.1099/ijs.0.68146-0
copyright statement
I have transferred the copyright for this publication to the publisher
id
1217433
handle
http://hdl.handle.net/1854/LU-1217433
date created
2011-05-05 11:16:02
date last changed
2013-04-22 15:56:23
@article{1217433,
  abstract     = {Three Gram-negative, microaerophilic bacteria, strains ASB1(T), ASB2 and ASB3, with a corkscrew-like morphology isolated from the gastric mucosa of cats were studied using a polyphasic taxonomic approach. The isolates grew on biphasic culture plates under microaerobic conditions at 37 degrees C and exhibited urease, oxidase and catalase activities. They were also able to grow in colonies on dry agar plates. Based on 16S rRNA gene sequence analysis, ASB1(T), ASB2 and ASB3 were identified as members of the genus Helicobacter and showed 98 to 99\% sequence similarity to strains of Helicobacter felis, Helicobacter bizzozeronii, 'Candidatus Helicobacter heilmannii', Helicobacter cynogastricus, Helicobacter baculiformis and Helicobacter salomonis, six related Helicobacter species previously detected in feline or canine gastric mucosa. Sequencing of the partial hsp60 gene demonstrated that ASB1(T), ASB2 and ASB3 constitute a separate taxon among the feline and canine Helicobacter species. The urease gene sequences of ASB1(T), ASB2 and ASB3 showed approximately 91\% similarity to those of 'Candidatus Helicobacter heilmannii'. Protein profiling, the absence of alkaline phosphatase activity and several other biochemical characteristics also allowed strains ASB1(T), ASB2 and ASB3 to be differentiated from other Helicobacter species of feline or canine gastric origin. The results of this polyphasic taxonomic study show that the cultured isolates constitute a new taxon corresponding to 'Candidatus Helicobacter heilmannii', which was previously demonstrated in the stomach of humans, wild felidae, cats and dogs. The name Helicobacter heilmannii sp. nov. is proposed for these isolates; the type strain is ASB1(T) (=DSM 23983(T)=LMG 26292(T)).},
  author       = {Smet, Annemieke and Flahou, Bram and D'Herde, Katharina and Vandamme, Peter and Cleenwerck, Ilse and Ducatelle, Richard and Pasmans, Frank and Haesebrouck, Freddy},
  issn         = {1466-5026},
  journal      = {INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY},
  keyword      = {CATS,PREVALENCE,ORGANISMS,BIZZOZERONII,IDENTIFICATION,DOMESTIC-ANIMALS,CANINE,SAMPLES,INFECTION,STOMACH},
  language     = {eng},
  number       = {2},
  pages        = {299--306},
  title        = {Helicobacter heilmannii sp. nov., isolated from feline gastric mucosa},
  url          = {http://dx.doi.org/10.1099/ijs.0.029207-0},
  volume       = {62},
  year         = {2012},
}

Chicago
Smet, Annemieke, Bram Flahou, Katharina D’Herde, Peter Vandamme, Ilse Cleenwerck, Richard Ducatelle, Frank Pasmans, and Freddy Haesebrouck. 2012. “Helicobacter Heilmannii Sp. Nov., Isolated from Feline Gastric Mucosa.” International Journal of Systematic and Evolutionary Microbiology 62 (2): 299–306.
APA
Smet, A., Flahou, B., D’Herde, K., Vandamme, P., Cleenwerck, I., Ducatelle, R., Pasmans, F., et al. (2012). Helicobacter heilmannii sp. nov., isolated from feline gastric mucosa. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 62(2), 299–306.
Vancouver
1.
Smet A, Flahou B, D’Herde K, Vandamme P, Cleenwerck I, Ducatelle R, et al. Helicobacter heilmannii sp. nov., isolated from feline gastric mucosa. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY. 2012;62(2):299–306.
MLA
Smet, Annemieke, Bram Flahou, Katharina D’Herde, et al. “Helicobacter Heilmannii Sp. Nov., Isolated from Feline Gastric Mucosa.” INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 62.2 (2012): 299–306. Print.