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DNA stress checkpoint control in Arabidopsis thaliana by WEE1

Toon Cools UGent (2011)
abstract
The WEE1 kinase is a conserved cell cycle inhibitor that, together with CDC25, controls CDK activity and consequently the G2/M progression in yeast and animals. The same control mechanism is utilized by the DNA damage checkpoint to induce a cell cycle arrest upon DNA stress. In plants, a homolog of the WEE1 gene is present and is also responsible for phosphorylation of CDKs, although only during DNA damage. Plants that not contain a functional WEE1 gene develop normally during normal growth circumstances, but show a hypersensitive growth response to replication stress. To understand this, a transcriptomics analysis was performed, indicating prolonged S-phase duration. A role for WEE1 during S phase was substantiated by its specific accumulation in replicating nuclei that suffered from DNA stress. Besides an extended replication phase, WEE1 knockout plants accumulated dead cells that were associated with the onset of premature vascular differentiation. Furthermore, a new synchronization method for Arabidopsis roots was presented and the initial research on the posttranslational control of plant WEE1 was executed, both providing intriguing results.
Please use this url to cite or link to this publication:
author
promoter
UGent and UGent
organization
year
type
dissertation (monograph)
subject
keyword
vascular differentiation, synchronization, checkpoints, DNA damage, WEE1, cell cycle
pages
VI, 190 pages
publisher
Ghent University. Faculty of Sciences
place of publication
Ghent, Belgium
defense location
Zwijnaarde : Technologiepark (FSVM building)
defense date
2011-04-29 16:00
language
English
UGent publication?
yes
classification
D1
additional info
dissertation consists of copyrighted material
copyright statement
I have transferred the copyright for this publication to the publisher
id
1216535
handle
http://hdl.handle.net/1854/LU-1216535
date created
2011-05-03 17:26:36
date last changed
2011-05-04 09:14:12
@phdthesis{1216535,
  abstract     = {The WEE1 kinase is a conserved cell cycle inhibitor that, together with CDC25, controls CDK activity and consequently the G2/M progression in yeast and animals. The same control mechanism is utilized by the DNA damage checkpoint to induce a cell cycle arrest upon DNA stress. In plants, a homolog of the WEE1 gene is present and is also responsible for phosphorylation of CDKs, although only during DNA damage. Plants that not contain a functional WEE1 gene develop normally during normal growth circumstances, but show a hypersensitive growth response to replication stress. To understand this, a transcriptomics analysis was performed, indicating prolonged S-phase duration. A role for WEE1 during S phase was substantiated by its specific accumulation in replicating nuclei that suffered from DNA stress. Besides an extended replication phase, WEE1 knockout plants accumulated dead cells that were associated with the onset of premature vascular differentiation. Furthermore, a new synchronization method for Arabidopsis roots was presented and the initial research on the posttranslational control of plant WEE1 was executed, both providing intriguing results.},
  author       = {Cools, Toon},
  keyword      = {vascular differentiation,synchronization,checkpoints,DNA damage,WEE1,cell cycle},
  language     = {eng},
  pages        = {VI, 190},
  publisher    = {Ghent University. Faculty of Sciences},
  school       = {Ghent University},
  title        = {DNA stress checkpoint control in Arabidopsis thaliana by WEE1},
  year         = {2011},
}

Chicago
Cools, Toon. 2011. “DNA Stress Checkpoint Control in Arabidopsis Thaliana by WEE1”. Ghent, Belgium: Ghent University. Faculty of Sciences.
APA
Cools, T. (2011). DNA stress checkpoint control in Arabidopsis thaliana by WEE1. Ghent University. Faculty of Sciences, Ghent, Belgium.
Vancouver
1.
Cools T. DNA stress checkpoint control in Arabidopsis thaliana by WEE1. [Ghent, Belgium]: Ghent University. Faculty of Sciences; 2011.
MLA
Cools, Toon. “DNA Stress Checkpoint Control in Arabidopsis Thaliana by WEE1.” 2011 : n. pag. Print.