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The micronucleus assay in radiation accidents

Hubert Thierens (UGent) and Anne Vral (UGent)
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Abstract
The cytokinesis-block micronucleus assay in peripheral blood lymphocytes is a standardised and validated technique for biodosimetry. Automated scoring of micronuclei allows large scale applications as in population triage in case of radiation accidents or malevolent use of radioactive sources. The dose detection limit (95% confidence) of the micronucleus assay for individual dose assessment is restricted to 0.2 Gy but can be decreased to 0.1 Gy by scoring centromeres in micronuclei using fluorescence in situ hybridization (FISH). In the past the micronucleus assay was applied for a number of large scale biomonitoring studies of nuclear power plant workers and hospital workers. Baseline micronucleus frequencies depend strongly on age and gender. The assay was also already used for biodosimetry of radiation accidents. In a multiple endpoint biodosimetry study for dose assessment of a worker exposed accidentally in 2003 to X-rays, a good agreement was obtained between dose estimates resulting from the micronucleus assay, the scoring of dicentrics and translocations. Automated scoring of micronuclei in combination with centromere signals, allowing systematic biodosimetry of exposed populations, remains a challenge for the future.
Keywords
micronucleus assay, radiation accidents, biodosimetry

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Citation

Please use this url to cite or link to this publication:

Chicago
Thierens, Hubert, and Anne Vral. 2009. “The Micronucleus Assay in Radiation Accidents.” Annali Dell Istituto Superiore Di Sanita 45 (3): 260–264.
APA
Thierens, H., & Vral, A. (2009). The micronucleus assay in radiation accidents. ANNALI DELL ISTITUTO SUPERIORE DI SANITA, 45(3), 260–264.
Vancouver
1.
Thierens H, Vral A. The micronucleus assay in radiation accidents. ANNALI DELL ISTITUTO SUPERIORE DI SANITA. 2009;45(3):260–4.
MLA
Thierens, Hubert, and Anne Vral. “The Micronucleus Assay in Radiation Accidents.” ANNALI DELL ISTITUTO SUPERIORE DI SANITA 45.3 (2009): 260–264. Print.
@article{1215443,
  abstract     = {The cytokinesis-block micronucleus assay in peripheral blood lymphocytes is a standardised and validated technique for biodosimetry. Automated scoring of micronuclei allows large scale applications as in population triage in case of radiation accidents or malevolent use of radioactive sources. The dose detection limit (95\% confidence) of the micronucleus assay for individual dose assessment is restricted to 0.2 Gy but can be decreased to 0.1 Gy by scoring centromeres in micronuclei using fluorescence in situ hybridization (FISH). In the past the micronucleus assay was applied for a number of large scale biomonitoring studies of nuclear power plant workers and hospital workers. Baseline micronucleus frequencies depend strongly on age and gender. The assay was also already used for biodosimetry of radiation accidents. In a multiple endpoint biodosimetry study for dose assessment of a worker exposed accidentally in 2003 to X-rays, a good agreement was obtained between dose estimates resulting from the micronucleus assay, the scoring of dicentrics and translocations. Automated scoring of micronuclei in combination with centromere signals, allowing systematic biodosimetry of exposed populations, remains a challenge for the future.},
  author       = {Thierens, Hubert and Vral, Anne},
  issn         = {0021-2571},
  journal      = {ANNALI DELL ISTITUTO SUPERIORE DI SANITA},
  keyword      = {micronucleus assay,radiation accidents,biodosimetry},
  language     = {eng},
  number       = {3},
  pages        = {260--264},
  title        = {The micronucleus assay in radiation accidents},
  volume       = {45},
  year         = {2009},
}

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