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Post-plasma grafting of AEMA as a versatile tool to biofunctionalise polyesters for tissue engineering

Tim Desmet UGent, Thomas Billiet UGent, Elke Berneel UGent, Maria Cornelissen UGent, David Schaubroeck UGent, Etienne Schacht UGent and Peter Dubruel UGent (2010) MACROMOLECULAR BIOSCIENCE. 10(12). p.1484-1494
abstract
In the last decade, substantial research in the field of post-plasma grafting surface modification has focussed on the introduction of carboxylic acids on surfaces by grafting acrylic acid (AAc). In the present work, we report on an alternative approach for biomaterial surface functionalisation. Thin poly-epsilon-caprolactone (PCL) films were subjected to a dielectric barrier discharge Ar-plasma followed by the grafting of 2-aminoethyl methacrylate (AEMA) under UV-irradiation. X-ray photoelectron spectroscopy (XPS) confirmed the presence of nitrogen. The ninhydrin assay demonstrated, both quantitatively and qualitatively, the presence of free amines on the surface. Confocal fluorescence microscopy (CFM), atomic force microscopy (AFM) and scanning electron microscopy (SEM) were used to visualise the grafted surfaces, indicating the presence of pAEMA. Static contact angle (SCA) measurements indicated a permanent increase in hydrophilicity. Furthermore, the AEMA grafted surfaces were applied for comparing the physisorption and covalent immobilisation of gelatin. CFM demonstrated that only the covalent immobilisation lead to a complete coverage of the surface. Those gelatin-coated surfaces obtained were further coated using fibronectin. Osteosarcoma cells demonstrated better cell-adhesion and cell-viability on the modified surfaces, compared to the pure PCL films.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
SYNTHETIC BIODEGRADABLE POLYMERS, POLY-EPSILON-CAPROLACTONE, AMINOLYZED POLY(L-LACTIC ACID), OF-THE-ART, SURFACE MODIFICATION, ACRYLIC-ACID, CELL-ADHESION, POLY(ETHYLENE-TEREPHTHALATE) FILMS, ENDOTHELIUM REGENERATION, MECHANICAL-PROPERTIES, surface modification, surface characterisation, poly(epsilon-caprolactone), gelatine, biocompatibility, films
journal title
MACROMOLECULAR BIOSCIENCE
Macromol. Biosci.
volume
10
issue
12
pages
1484 - 1494
Web of Science type
Article
Web of Science id
000285313000011
JCR category
POLYMER SCIENCE
JCR impact factor
3.458 (2010)
JCR rank
12/78 (2010)
JCR quartile
1 (2010)
ISSN
1616-5187
DOI
10.1002/mabi.201000147
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1212535
handle
http://hdl.handle.net/1854/LU-1212535
date created
2011-04-26 15:26:20
date last changed
2011-04-26 17:02:16
@article{1212535,
  abstract     = {In the last decade, substantial research in the field of post-plasma grafting surface modification has focussed on the introduction of carboxylic acids on surfaces by grafting acrylic acid (AAc). In the present work, we report on an alternative approach for biomaterial surface functionalisation. Thin poly-epsilon-caprolactone (PCL) films were subjected to a dielectric barrier discharge Ar-plasma followed by the grafting of 2-aminoethyl methacrylate (AEMA) under UV-irradiation. X-ray photoelectron spectroscopy (XPS) confirmed the presence of nitrogen. The ninhydrin assay demonstrated, both quantitatively and qualitatively, the presence of free amines on the surface. Confocal fluorescence microscopy (CFM), atomic force microscopy (AFM) and scanning electron microscopy (SEM) were used to visualise the grafted surfaces, indicating the presence of pAEMA. Static contact angle (SCA) measurements indicated a permanent increase in hydrophilicity. Furthermore, the AEMA grafted surfaces were applied for comparing the physisorption and covalent immobilisation of gelatin. CFM demonstrated that only the covalent immobilisation lead to a complete coverage of the surface. Those gelatin-coated surfaces obtained were further coated using fibronectin. Osteosarcoma cells demonstrated better cell-adhesion and cell-viability on the modified surfaces, compared to the pure PCL films.},
  author       = {Desmet, Tim and Billiet, Thomas and Berneel, Elke and Cornelissen, Maria and Schaubroeck, David and Schacht, Etienne and Dubruel, Peter},
  issn         = {1616-5187},
  journal      = {MACROMOLECULAR BIOSCIENCE},
  keyword      = {SYNTHETIC BIODEGRADABLE POLYMERS,POLY-EPSILON-CAPROLACTONE,AMINOLYZED POLY(L-LACTIC ACID),OF-THE-ART,SURFACE MODIFICATION,ACRYLIC-ACID,CELL-ADHESION,POLY(ETHYLENE-TEREPHTHALATE) FILMS,ENDOTHELIUM REGENERATION,MECHANICAL-PROPERTIES,surface modification,surface characterisation,poly(epsilon-caprolactone),gelatine,biocompatibility,films},
  language     = {eng},
  number       = {12},
  pages        = {1484--1494},
  title        = {Post-plasma grafting of AEMA as a versatile tool to biofunctionalise polyesters for tissue engineering},
  url          = {http://dx.doi.org/10.1002/mabi.201000147},
  volume       = {10},
  year         = {2010},
}

Chicago
Desmet, Tim, Thomas Billiet, Elke Berneel, Maria Cornelissen, David Schaubroeck, Etienne Schacht, and Peter Dubruel. 2010. “Post-plasma Grafting of AEMA as a Versatile Tool to Biofunctionalise Polyesters for Tissue Engineering.” Macromolecular Bioscience 10 (12): 1484–1494.
APA
Desmet, Tim, Billiet, T., Berneel, E., Cornelissen, M., Schaubroeck, D., Schacht, E., & Dubruel, P. (2010). Post-plasma grafting of AEMA as a versatile tool to biofunctionalise polyesters for tissue engineering. MACROMOLECULAR BIOSCIENCE, 10(12), 1484–1494.
Vancouver
1.
Desmet T, Billiet T, Berneel E, Cornelissen M, Schaubroeck D, Schacht E, et al. Post-plasma grafting of AEMA as a versatile tool to biofunctionalise polyesters for tissue engineering. MACROMOLECULAR BIOSCIENCE. 2010;10(12):1484–94.
MLA
Desmet, Tim, Thomas Billiet, Elke Berneel, et al. “Post-plasma Grafting of AEMA as a Versatile Tool to Biofunctionalise Polyesters for Tissue Engineering.” MACROMOLECULAR BIOSCIENCE 10.12 (2010): 1484–1494. Print.