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Chromosomal radiosensitivity of HIV positive individuals

Ans Baeyens, Jacobus P Slabbert, Pascale Willem, Sibusiso Jozela, Debby Van Der Merwe and Anne Vral UGent (2010) INTERNATIONAL JOURNAL OF RADIATION BIOLOGY. 86(7). p.584-592
abstract
Purpose: Radiosensitivity in relation to the human immunodeficiency virus (HIV) status is important in South Africa as the prevalence of HIV infections is high. In this study the in vitro chromosomal radiosensitivity of HIV positive individuals was investigated and compared with that of HIV negative individuals. Materials and methods: Blood samples from 59 HIV positive and 39 HIV negative individuals were exposed in vitro to doses of 6MV X-rays ranging from 1-4 Gy. Chromosomal radiosensitivity was assessed with the micronucleus assay. Micronuclei are a measure of chromosomal damage and were quantified in at least 500 binucleated lymphoblasts (BN) per sample. Un-irradiated control samples from each donor were also analysed. Results: In 47% of HIV positive individuals difficulties with cell stimulation by adding phytohaemagglutinin (PHA) to blood cultures were noticed which resulted in insufficient yield of BN for microscopic analysis. Micronuclei frequencies were consistently higher in irradiated lymphocytes obtained from HIV positive individuals compared to that observed in cells from HIV negative donors. Data for both groups were fitted to the linear-quadratic equation Y = alpha D + beta D-2 where Y is the number of micronuclei in 500 binucleated cells and D is the dose in Gy. The fitted parameters for respectively HIV positive and HIV negative lymphocytes are alpha -80.17 Gy(-1), beta - 14 Gy(-2) and alpha - 54.5 Gy(-1), beta - 16.2 Gy(-2). The confidence ellipses of these parameters are separated indicating that the increase in radiosensitivity is statistically significant. Conclusion: T-lymphocytes of HIV infected individuals were considerably more sensitive to X-rays compared to that of HIV negative donors. This may have implications for normal tissue tolerance during radiotherapy as well as for the radiological health of radiation workers.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
the human immunedeficiency virus (HIV), dose response curve, chromosomal radiosensitivity, micronucleus (MN) assay, HUMAN LYMPHOCYTE SUBPOPULATIONS, MICRONUCLEUS-CENTROMERE ASSAY, PERIPHERAL-BLOOD LYMPHOCYTES, RADIATION-INDUCED APOPTOSIS, NATURAL-KILLER-CELLS, NORMAL TISSUE-DAMAGE, DOUBLE-STRAND BREAKS, KAPOSIS-SARCOMA, AIDS PATIENTS, HUMAN-IMMUNODEFICIENCY-VIRUS
journal title
INTERNATIONAL JOURNAL OF RADIATION BIOLOGY
Int. J. Radiat. Biol.
volume
86
issue
7
pages
584 - 592
Web of Science type
Article
Web of Science id
000279507400007
JCR category
NUCLEAR SCIENCE & TECHNOLOGY
JCR impact factor
1.861 (2010)
JCR rank
2/34 (2010)
JCR quartile
1 (2010)
ISSN
0955-3002
DOI
10.3109/09553001003734576
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1212438
handle
http://hdl.handle.net/1854/LU-1212438
date created
2011-04-26 14:19:15
date last changed
2011-04-26 14:49:32
@article{1212438,
  abstract     = {Purpose: Radiosensitivity in relation to the human immunodeficiency virus (HIV) status is important in South Africa as the prevalence of HIV infections is high. In this study the in vitro chromosomal radiosensitivity of HIV positive individuals was investigated and compared with that of HIV negative individuals.
Materials and methods: Blood samples from 59 HIV positive and 39 HIV negative individuals were exposed in vitro to doses of 6MV X-rays ranging from 1-4 Gy. Chromosomal radiosensitivity was assessed with the micronucleus assay. Micronuclei are a measure of chromosomal damage and were quantified in at least 500 binucleated lymphoblasts (BN) per sample. Un-irradiated control samples from each donor were also analysed.
Results: In 47\% of HIV positive individuals difficulties with cell stimulation by adding phytohaemagglutinin (PHA) to blood cultures were noticed which resulted in insufficient yield of BN for microscopic analysis. Micronuclei frequencies were consistently higher in irradiated lymphocytes obtained from HIV positive individuals compared to that observed in cells from HIV negative donors. Data for both groups were fitted to the linear-quadratic equation Y = alpha D + beta D-2 where Y is the number of micronuclei in 500 binucleated cells and D is the dose in Gy. The fitted parameters for respectively HIV positive and HIV negative lymphocytes are alpha -80.17 Gy(-1), beta - 14 Gy(-2) and alpha - 54.5 Gy(-1), beta - 16.2 Gy(-2). The confidence ellipses of these parameters are separated indicating that the increase in radiosensitivity is statistically significant.
Conclusion: T-lymphocytes of HIV infected individuals were considerably more sensitive to X-rays compared to that of HIV negative donors. This may have implications for normal tissue tolerance during radiotherapy as well as for the radiological health of radiation workers.},
  author       = {Baeyens, Ans and Slabbert, Jacobus P and Willem, Pascale and Jozela, Sibusiso and Van Der Merwe, Debby and Vral, Anne},
  issn         = {0955-3002},
  journal      = {INTERNATIONAL JOURNAL OF RADIATION BIOLOGY},
  keyword      = {the human immunedeficiency virus (HIV),dose response curve,chromosomal radiosensitivity,micronucleus (MN) assay,HUMAN LYMPHOCYTE SUBPOPULATIONS,MICRONUCLEUS-CENTROMERE ASSAY,PERIPHERAL-BLOOD LYMPHOCYTES,RADIATION-INDUCED APOPTOSIS,NATURAL-KILLER-CELLS,NORMAL TISSUE-DAMAGE,DOUBLE-STRAND BREAKS,KAPOSIS-SARCOMA,AIDS PATIENTS,HUMAN-IMMUNODEFICIENCY-VIRUS},
  language     = {eng},
  number       = {7},
  pages        = {584--592},
  title        = {Chromosomal radiosensitivity of HIV positive individuals},
  url          = {http://dx.doi.org/10.3109/09553001003734576},
  volume       = {86},
  year         = {2010},
}

Chicago
Baeyens, Ans, Jacobus P Slabbert, Pascale Willem, Sibusiso Jozela, Debby Van Der Merwe, and Anne Vral. 2010. “Chromosomal Radiosensitivity of HIV Positive Individuals.” International Journal of Radiation Biology 86 (7): 584–592.
APA
Baeyens, A., Slabbert, J. P., Willem, P., Jozela, S., Van Der Merwe, D., & Vral, A. (2010). Chromosomal radiosensitivity of HIV positive individuals. INTERNATIONAL JOURNAL OF RADIATION BIOLOGY, 86(7), 584–592.
Vancouver
1.
Baeyens A, Slabbert JP, Willem P, Jozela S, Van Der Merwe D, Vral A. Chromosomal radiosensitivity of HIV positive individuals. INTERNATIONAL JOURNAL OF RADIATION BIOLOGY. 2010;86(7):584–92.
MLA
Baeyens, Ans, Jacobus P Slabbert, Pascale Willem, et al. “Chromosomal Radiosensitivity of HIV Positive Individuals.” INTERNATIONAL JOURNAL OF RADIATION BIOLOGY 86.7 (2010): 584–592. Print.