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The Arabidopsis bHLH transcription factors MYC3 and MYC4 are targets of JAZ repressors and act additively with MYC2 in the activation of jasmonate responses

Patricia Fernández-Calvo, Andrea Chini, Gemma Fernández-Barbero, José-Manuel Chico, Selena Gímenez-Ibanez, Jan Geerinck UGent, Dominique Eeckhout UGent, Fabian Schweizer, Marta Godoy and José Manuel Franco-Zorrilla, et al. (2011) PLANT CELL. 23(2). p.701-715
abstract
Jasmonates (JAs) trigger an important transcriptional reprogramming of plant cells to modulate both basal development and stress responses. In spite of the importance of transcriptional regulation, only one transcription factor (TF), the Arabidopsis thaliana basic helix-loop-helix MYC2, has been described so far as a direct target of JAZ repressors. By means of yeast two-hybrid screening and tandem affinity purification strategies, we identified two previously unknown targets of JAZ repressors, the TFs MYC3 and MYC4, phylogenetically closely related to MYC2. We show that MYC3 and MYC4 interact in vitro and in vivo with JAZ repressors and also form homo-and heterodimers with MYC2 and among themselves. They both are nuclear proteins that bind DNA with sequence specificity similar to that of MYC2. Loss-of-function mutations in any of these two TFs impair full responsiveness to JA and enhance the JA insensitivity of myc2 mutants. Moreover, the triple mutant myc2 myc3 myc4 is as impaired as coi1-1 in the activation of several, but not all, JA-mediated responses such as the defense against bacterial pathogens and insect herbivory. Our results show that MYC3 and MYC4 are activators of JA-regulated programs that act additively with MYC2 to regulate specifically different subsets of the JA-dependent transcriptional response.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
GENE, CORONATINE, PROTEINS, THALIANA, AUXIN RECEPTORS, PLANT DEVELOPMENT, PSEUDOMONAS-SYRINGAE, REGULATED DEFENSE, TANDEM AFFINITY PURIFICATION, METHYL JASMONATE
journal title
PLANT CELL
Plant Cell
volume
23
issue
2
pages
701 - 715
Web of Science type
Article
Web of Science id
000288801000019
JCR category
PLANT SCIENCES
JCR impact factor
8.987 (2011)
JCR rank
5/189 (2011)
JCR quartile
1 (2011)
ISSN
1040-4651
DOI
10.1105/tpc.110.080788
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1209242
handle
http://hdl.handle.net/1854/LU-1209242
date created
2011-04-14 18:20:10
date last changed
2012-12-17 16:46:26
@article{1209242,
  abstract     = {Jasmonates (JAs) trigger an important transcriptional reprogramming of plant cells to modulate both basal development and stress responses. In spite of the importance of transcriptional regulation, only one transcription factor (TF), the Arabidopsis thaliana basic helix-loop-helix MYC2, has been described so far as a direct target of JAZ repressors. By means of yeast two-hybrid screening and tandem affinity purification strategies, we identified two previously unknown targets of JAZ repressors, the TFs MYC3 and MYC4, phylogenetically closely related to MYC2. We show that MYC3 and MYC4 interact in vitro and in vivo with JAZ repressors and also form homo-and heterodimers with MYC2 and among themselves. They both are nuclear proteins that bind DNA with sequence specificity similar to that of MYC2. Loss-of-function mutations in any of these two TFs impair full responsiveness to JA and enhance the JA insensitivity of myc2 mutants. Moreover, the triple mutant myc2 myc3 myc4 is as impaired as coi1-1 in the activation of several, but not all, JA-mediated responses such as the defense against bacterial pathogens and insect herbivory. Our results show that MYC3 and MYC4 are activators of JA-regulated programs that act additively with MYC2 to regulate specifically different subsets of the JA-dependent transcriptional response.},
  author       = {Fern{\'a}ndez-Calvo, Patricia and Chini, Andrea and Fern{\'a}ndez-Barbero, Gemma and Chico, Jos{\'e}-Manuel and G{\'i}menez-Ibanez, Selena and Geerinck, Jan and Eeckhout, Dominique and Schweizer, Fabian and Godoy, Marta and Franco-Zorrilla, Jos{\'e} Manuel and Pauwels, Laurens and Witters, Erwin and Puga, Mar{\'i}a Isabel and Paz-Ares, Javier and Goossens, Alain and Reymond, Philippe and De Jaeger, Geert and Solano, Roberto},
  issn         = {1040-4651},
  journal      = {PLANT CELL},
  keyword      = {GENE,CORONATINE,PROTEINS,THALIANA,AUXIN RECEPTORS,PLANT DEVELOPMENT,PSEUDOMONAS-SYRINGAE,REGULATED DEFENSE,TANDEM AFFINITY PURIFICATION,METHYL JASMONATE},
  language     = {eng},
  number       = {2},
  pages        = {701--715},
  title        = {The Arabidopsis bHLH transcription factors MYC3 and MYC4 are targets of JAZ repressors and act additively with MYC2 in the activation of jasmonate responses},
  url          = {http://dx.doi.org/10.1105/tpc.110.080788},
  volume       = {23},
  year         = {2011},
}

Chicago
Fernández-Calvo, Patricia, Andrea Chini, Gemma Fernández-Barbero, José-Manuel Chico, Selena Gímenez-Ibanez, Jan Geerinck, Dominique Eeckhout, et al. 2011. “The Arabidopsis bHLH Transcription Factors MYC3 and MYC4 Are Targets of JAZ Repressors and Act Additively with MYC2 in the Activation of Jasmonate Responses.” Plant Cell 23 (2): 701–715.
APA
Fernández-Calvo, P., Chini, A., Fernández-Barbero, G., Chico, J.-M., Gímenez-Ibanez, S., Geerinck, J., Eeckhout, D., et al. (2011). The Arabidopsis bHLH transcription factors MYC3 and MYC4 are targets of JAZ repressors and act additively with MYC2 in the activation of jasmonate responses. PLANT CELL, 23(2), 701–715.
Vancouver
1.
Fernández-Calvo P, Chini A, Fernández-Barbero G, Chico J-M, Gímenez-Ibanez S, Geerinck J, et al. The Arabidopsis bHLH transcription factors MYC3 and MYC4 are targets of JAZ repressors and act additively with MYC2 in the activation of jasmonate responses. PLANT CELL. 2011;23(2):701–15.
MLA
Fernández-Calvo, Patricia, Andrea Chini, Gemma Fernández-Barbero, et al. “The Arabidopsis bHLH Transcription Factors MYC3 and MYC4 Are Targets of JAZ Repressors and Act Additively with MYC2 in the Activation of Jasmonate Responses.” PLANT CELL 23.2 (2011): 701–715. Print.