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A Reciprocal N-15-labeling proteomic analysis of expanding arabidopsis leaves subjected to osmotic stress indicates importance of mitochondria in preserving plastid functions

Aleksandra Skirycz UGent, Samy Memmi UGent, Stefanie De Bodt UGent, Katrien Maleux UGent, Toshihiro Obata, Alisdai R Fernie, Bart Devreese UGent and Dirk Inzé UGent (2011) JOURNAL OF PROTEOME RESEARCH. 10(3). p.1018-1029
abstract
Plants respond to environmental stress by dynamically reprogramming their growth. Whereas stress onset is accompanied by rapid growth inhibition leading to smaller organs, growth will recover and adapt once the stress conditions become stable and do no threaten plant survival. Here, adaptation of growing Arabidopsis thaliana leaves to mild and prolonged osmotic stress was investigated by means of a complete metabolic labeling strategy with the N-15-stable isotope as a complement to a previously published transcript and metabolite profiling. Global analysis of protein changes revealed that plastidial ATPase, Calvin cycle, and photorespiration were down-regulated, but mitochondrial ATP synthesis was up-regulated, indicating the importance of mitochondria in preserving plastid functions during water stress. Although transcript and protein data correlated well with the stable and prolonged character of the applied stress, numerous proteins were clearly regulated at the post-transcriptional level that could, at least partly, be related to changes in protein synthesis and degradation. In conclusion, proteomics using the N-15 labeling helped understand the mechanisms underlying growth adaptation to osmotic stress and allowed the identification of candidate genes to improve plant growth under limited water.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
QUANTITATIVE PROTEOMICS, OXIDATIVE STRESS, proteome, ROS scavenging, ATP, mitochondria, respiration, photosynthesis, osmotic stress, leaf growth, Arabidopsis, Metabolic labeling, PLANT-MITOCHONDRIA, ATP SYNTHASE, BACTERIAL EXPRESSION, METABOLIC-RESPONSE, MASS-SPECTROMETRY, ABIOTIC STRESSES, GENE-EXPRESSION, LEAF PROTEOME
journal title
JOURNAL OF PROTEOME RESEARCH
J. Proteome Res.
volume
10
issue
3
pages
1018 - 1029
Web of Science type
Article
Web of Science id
000287944000009
JCR category
BIOCHEMICAL RESEARCH METHODS
JCR impact factor
5.113 (2011)
JCR rank
10/72 (2011)
JCR quartile
1 (2011)
ISSN
1535-3893
DOI
10.1021/pr100785n
project
Biotechnology for a sustainable economy (Bio-Economy)
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1209205
handle
http://hdl.handle.net/1854/LU-1209205
date created
2011-04-14 18:19:39
date last changed
2013-02-27 12:06:03
@article{1209205,
  abstract     = {Plants respond to environmental stress by dynamically reprogramming their growth. Whereas stress onset is accompanied by rapid growth inhibition leading to smaller organs, growth will recover and adapt once the stress conditions become stable and do no threaten plant survival. Here, adaptation of growing Arabidopsis thaliana leaves to mild and prolonged osmotic stress was investigated by means of a complete metabolic labeling strategy with the N-15-stable isotope as a complement to a previously published transcript and metabolite profiling. Global analysis of protein changes revealed that plastidial ATPase, Calvin cycle, and photorespiration were down-regulated, but mitochondrial ATP synthesis was up-regulated, indicating the importance of mitochondria in preserving plastid functions during water stress. Although transcript and protein data correlated well with the stable and prolonged character of the applied stress, numerous proteins were clearly regulated at the post-transcriptional level that could, at least partly, be related to changes in protein synthesis and degradation. In conclusion, proteomics using the N-15 labeling helped understand the mechanisms underlying growth adaptation to osmotic stress and allowed the identification of candidate genes to improve plant growth under limited water.},
  author       = {Skirycz, Aleksandra and Memmi, Samy and De Bodt, Stefanie and Maleux, Katrien and Obata, Toshihiro and Fernie, Alisdai R and Devreese, Bart and Inz{\'e}, Dirk},
  issn         = {1535-3893},
  journal      = {JOURNAL OF PROTEOME RESEARCH},
  keyword      = {QUANTITATIVE PROTEOMICS,OXIDATIVE STRESS,proteome,ROS scavenging,ATP,mitochondria,respiration,photosynthesis,osmotic stress,leaf growth,Arabidopsis,Metabolic labeling,PLANT-MITOCHONDRIA,ATP SYNTHASE,BACTERIAL EXPRESSION,METABOLIC-RESPONSE,MASS-SPECTROMETRY,ABIOTIC STRESSES,GENE-EXPRESSION,LEAF PROTEOME},
  language     = {eng},
  number       = {3},
  pages        = {1018--1029},
  title        = {A Reciprocal N-15-labeling proteomic analysis of expanding arabidopsis leaves subjected to osmotic stress indicates importance of mitochondria in preserving plastid functions},
  url          = {http://dx.doi.org/10.1021/pr100785n},
  volume       = {10},
  year         = {2011},
}

Chicago
Skirycz, Aleksandra, Samy Memmi, Stefanie De Bodt, Katrien Maleux, Toshihiro Obata, Alisdai R Fernie, Bart Devreese, and Dirk Inzé. 2011. “A Reciprocal N-15-labeling Proteomic Analysis of Expanding Arabidopsis Leaves Subjected to Osmotic Stress Indicates Importance of Mitochondria in Preserving Plastid Functions.” Journal of Proteome Research 10 (3): 1018–1029.
APA
Skirycz, A., Memmi, S., De Bodt, S., Maleux, K., Obata, T., Fernie, A. R., Devreese, B., et al. (2011). A Reciprocal N-15-labeling proteomic analysis of expanding arabidopsis leaves subjected to osmotic stress indicates importance of mitochondria in preserving plastid functions. JOURNAL OF PROTEOME RESEARCH, 10(3), 1018–1029.
Vancouver
1.
Skirycz A, Memmi S, De Bodt S, Maleux K, Obata T, Fernie AR, et al. A Reciprocal N-15-labeling proteomic analysis of expanding arabidopsis leaves subjected to osmotic stress indicates importance of mitochondria in preserving plastid functions. JOURNAL OF PROTEOME RESEARCH. 2011;10(3):1018–29.
MLA
Skirycz, Aleksandra, Samy Memmi, Stefanie De Bodt, et al. “A Reciprocal N-15-labeling Proteomic Analysis of Expanding Arabidopsis Leaves Subjected to Osmotic Stress Indicates Importance of Mitochondria in Preserving Plastid Functions.” JOURNAL OF PROTEOME RESEARCH 10.3 (2011): 1018–1029. Print.