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Combining enhanced root and shoot growth reveals cross talk between pathways that control plant organ size in Arabidopsis

Liesbeth Vercruyssen UGent, Nathalie Gonzalez Sanchez UGent, Tomas Werner, Thomas Schmulling and Dirk Inzé UGent (2011) PLANT PHYSIOLOGY. 155(3). p.1339-1352
abstract
Functionally distinct Arabidopsis (Arabidopsis thaliana) genes that positively affect root or shoot growth when ectopically expressed were combined to explore the feasibility of enhanced biomass production. Enhanced root growth resulting from cytokinin deficiency was obtained by overexpressing CYTOKININ OXIDASE/DEHYDROGENASE3 (CKX3) under the control of the root-specific PYK10 promoter. Plants harboring the PYK10-CKX3 construct were crossed with four different transgenic lines showing enhanced leaf growth. For all combinations, the phenotypic traits of the individual lines could be combined, resulting in an overall growth increase. Unexpectedly, three out of four combinations had more than additive effects. Both leaf and root growth were synergistically enhanced in plants ectopically expressing CKX3 and BRASSINOSTEROID INSENSITIVE1, indicating cross talk between cytokinins and brassinosteroids. In agreement, treatment of PYK10-CKX3 plants with brassinolide resulted in a dramatic increase in lateral root growth that could not be observed in wild-type plants. Coexpression of CKX3 and the GROWTH-REGULATING FACTOR5 (GRF5) antagonized the effects of GRF5 overexpression, revealing an interplay between cytokinins and GRF5 during leaf cell proliferation. The combined overexpression of CKX3 and GIBBERELLIN 20-OXIDASE1 led to a synergistic increase in leaf growth, suggesting an antagonistic growth control by cytokinins and gibberellins. Only additive effects on root and shoot growth were visible in plants ectopically expressing both CKX3 and ARABIDOPSIS VACUOLAR PYROPHOSPHATASE1, hinting at an independent action mode. Our results show new interactions and contribute to the molecular and physiological understanding of biomass production at the whole plant level.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
BREVIS-RADIX, SALT TOLERANCE, FOUNDER CELLS, APICAL MERISTEM, CYTOKININ METABOLISM, CELL-PROLIFERATION, GIBBERELLIN 20-OXIDASE, GENE-EXPRESSION, HETEROLOGOUS EXPRESSION, H+-PPASE
journal title
PLANT PHYSIOLOGY
Plant Physiol.
volume
155
issue
3
pages
1339 - 1352
Web of Science type
Article
Web of Science id
000287843800024
JCR category
PLANT SCIENCES
JCR impact factor
6.535 (2011)
JCR rank
7/189 (2011)
JCR quartile
1 (2011)
ISSN
0032-0889
DOI
10.1104/pp.110.167049
project
Biotechnology for a sustainable economy (Bio-Economy)
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1209198
handle
http://hdl.handle.net/1854/LU-1209198
date created
2011-04-14 18:19:33
date last changed
2013-02-27 12:05:20
@article{1209198,
  abstract     = {Functionally distinct Arabidopsis (Arabidopsis thaliana) genes that positively affect root or shoot growth when ectopically expressed were combined to explore the feasibility of enhanced biomass production. Enhanced root growth resulting from cytokinin deficiency was obtained by overexpressing CYTOKININ OXIDASE/DEHYDROGENASE3 (CKX3) under the control of the root-specific PYK10 promoter. Plants harboring the PYK10-CKX3 construct were crossed with four different transgenic lines showing enhanced leaf growth. For all combinations, the phenotypic traits of the individual lines could be combined, resulting in an overall growth increase. Unexpectedly, three out of four combinations had more than additive effects. Both leaf and root growth were synergistically enhanced in plants ectopically expressing CKX3 and BRASSINOSTEROID INSENSITIVE1, indicating cross talk between cytokinins and brassinosteroids. In agreement, treatment of PYK10-CKX3 plants with brassinolide resulted in a dramatic increase in lateral root growth that could not be observed in wild-type plants. Coexpression of CKX3 and the GROWTH-REGULATING FACTOR5 (GRF5) antagonized the effects of GRF5 overexpression, revealing an interplay between cytokinins and GRF5 during leaf cell proliferation. The combined overexpression of CKX3 and GIBBERELLIN 20-OXIDASE1 led to a synergistic increase in leaf growth, suggesting an antagonistic growth control by cytokinins and gibberellins. Only additive effects on root and shoot growth were visible in plants ectopically expressing both CKX3 and ARABIDOPSIS VACUOLAR PYROPHOSPHATASE1, hinting at an independent action mode. Our results show new interactions and contribute to the molecular and physiological understanding of biomass production at the whole plant level.},
  author       = {Vercruyssen, Liesbeth and Gonzalez Sanchez, Nathalie and Werner, Tomas and Schmulling, Thomas and Inz{\'e}, Dirk},
  issn         = {0032-0889},
  journal      = {PLANT PHYSIOLOGY},
  keyword      = {BREVIS-RADIX,SALT TOLERANCE,FOUNDER CELLS,APICAL MERISTEM,CYTOKININ METABOLISM,CELL-PROLIFERATION,GIBBERELLIN 20-OXIDASE,GENE-EXPRESSION,HETEROLOGOUS EXPRESSION,H+-PPASE},
  language     = {eng},
  number       = {3},
  pages        = {1339--1352},
  title        = {Combining enhanced root and shoot growth reveals cross talk between pathways that control plant organ size in Arabidopsis},
  url          = {http://dx.doi.org/10.1104/pp.110.167049},
  volume       = {155},
  year         = {2011},
}

Chicago
Vercruyssen, Liesbeth, Nathalie Gonzalez Sanchez, Tomas Werner, Thomas Schmulling, and Dirk Inzé. 2011. “Combining Enhanced Root and Shoot Growth Reveals Cross Talk Between Pathways That Control Plant Organ Size in Arabidopsis.” Plant Physiology 155 (3): 1339–1352.
APA
Vercruyssen, L., Gonzalez Sanchez, N., Werner, T., Schmulling, T., & Inzé, D. (2011). Combining enhanced root and shoot growth reveals cross talk between pathways that control plant organ size in Arabidopsis. PLANT PHYSIOLOGY, 155(3), 1339–1352.
Vancouver
1.
Vercruyssen L, Gonzalez Sanchez N, Werner T, Schmulling T, Inzé D. Combining enhanced root and shoot growth reveals cross talk between pathways that control plant organ size in Arabidopsis. PLANT PHYSIOLOGY. 2011;155(3):1339–52.
MLA
Vercruyssen, Liesbeth, Nathalie Gonzalez Sanchez, Tomas Werner, et al. “Combining Enhanced Root and Shoot Growth Reveals Cross Talk Between Pathways That Control Plant Organ Size in Arabidopsis.” PLANT PHYSIOLOGY 155.3 (2011): 1339–1352. Print.