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Enzymatic glycosyl transfer: mechanisms and applications

Tom Desmet (UGent) and Wim Soetaert (UGent)
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Biotechnology for a sustainable economy (Bio-Economy)
Abstract
Glycosylated compounds have numerous applications in the food, pharmaceutical and personal care industries. Their synthesis is, however, far from trivial. Because chemical glycosylation reactions suffer from low yields and lack of selectivity, biocatalytic routes have received increasing attention as efficient and 'green' alternatives. Several types of biocatalysts can be used for this purpose, each with its own advantages and disadvantages. The applications of glycoside hydrolases, glycoside phosphorylases, transglycosidases and glycosyl transferases are discussed in light of the most recent insights into the mechanistic features of the enzymes. A thorough understanding of their structure function relationships should also allow a more efficient engineering of the activity and specificity of these enzymes. Several strategies to enhance the glycosylation potential of natural biocatalysts are presented.
Keywords
glycoside hydrolases, carbohydrate-active enzymes, Glycosylation, glycoside phosphorylases, transglycosidases, glycosyl transferases, RECOMBINANT SUCROSE PHOSPHORYLASE, NATURAL-PRODUCT GLYCOSYLTRANSFERASE, BACILLUS-SUBTILIS LEVANSUCRASE, SITE-DIRECTED MUTAGENESIS, D-GLUCOSE 1-PHOSPHATE, EGG-WHITE LYSOZYME, LEUCONOSTOC-MESENTEROIDES, OLIGOSACCHARIDE SYNTHESIS, CELLOBIOSE PHOSPHORYLASE, ACCEPTOR SPECIFICITY

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Citation

Please use this url to cite or link to this publication:

Chicago
Desmet, Tom, and Wim Soetaert. 2011. “Enzymatic Glycosyl Transfer: Mechanisms and Applications.” Biocatalysis and Biotransformation 29 (1): 1–18.
APA
Desmet, Tom, & Soetaert, W. (2011). Enzymatic glycosyl transfer: mechanisms and applications. BIOCATALYSIS AND BIOTRANSFORMATION, 29(1), 1–18.
Vancouver
1.
Desmet T, Soetaert W. Enzymatic glycosyl transfer: mechanisms and applications. BIOCATALYSIS AND BIOTRANSFORMATION. 2011;29(1):1–18.
MLA
Desmet, Tom, and Wim Soetaert. “Enzymatic Glycosyl Transfer: Mechanisms and Applications.” BIOCATALYSIS AND BIOTRANSFORMATION 29.1 (2011): 1–18. Print.
@article{1209002,
  abstract     = {Glycosylated compounds have numerous applications in the food, pharmaceutical and personal care industries. Their synthesis is, however, far from trivial. Because chemical glycosylation reactions suffer from low yields and lack of selectivity, biocatalytic routes have received increasing attention as efficient and 'green' alternatives. Several types of biocatalysts can be used for this purpose, each with its own advantages and disadvantages. The applications of glycoside hydrolases, glycoside phosphorylases, transglycosidases and glycosyl transferases are discussed in light of the most recent insights into the mechanistic features of the enzymes. A thorough understanding of their structure function relationships should also allow a more efficient engineering of the activity and specificity of these enzymes. Several strategies to enhance the glycosylation potential of natural biocatalysts are presented.},
  author       = {Desmet, Tom and Soetaert, Wim},
  issn         = {1024-2422},
  journal      = {BIOCATALYSIS AND BIOTRANSFORMATION},
  keywords     = {glycoside hydrolases,carbohydrate-active enzymes,Glycosylation,glycoside phosphorylases,transglycosidases,glycosyl transferases,RECOMBINANT SUCROSE PHOSPHORYLASE,NATURAL-PRODUCT GLYCOSYLTRANSFERASE,BACILLUS-SUBTILIS LEVANSUCRASE,SITE-DIRECTED MUTAGENESIS,D-GLUCOSE 1-PHOSPHATE,EGG-WHITE LYSOZYME,LEUCONOSTOC-MESENTEROIDES,OLIGOSACCHARIDE SYNTHESIS,CELLOBIOSE PHOSPHORYLASE,ACCEPTOR SPECIFICITY},
  language     = {eng},
  number       = {1},
  pages        = {1--18},
  title        = {Enzymatic glycosyl transfer: mechanisms and applications},
  url          = {http://dx.doi.org/10.3109/10242422.2010.548557},
  volume       = {29},
  year         = {2011},
}

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