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N-Acylhomoserine lactone quorum-sensing signalling in antagonistic phenazine-producing Pseudomonas isolates from the red cocoyam rhizosphere

Katrien De Maeyer UGent, Jolien D'aes UGent, Hoang Gia Khuong Hua UGent, Maaike Perneel, Lynn Vanhaecke UGent, Herlinde Noppe UGent and Monica Höfte UGent (2011) MICROBIOLOGY-SGM. 157(2). p.459-472
abstract
Forty fluorescent Pseudomonas strains isolated from white and red cocoyam roots were tested for their ability to synthesize N-acyl-L-homoserine lactones (acyl-HSLs). Remarkably, only isolates from the red cocoyam rhizosphere that were antagonistic against the cocoyam root rot pathogen Pythium myriotylum and synthesized phenazine antibiotics produced acyl-HSLs. This supports the assumption that acyl-HSL production is related to the antagonistic activity of the strains. After detection, the signal molecules were identified through TLC-overlay and liquid chromatography-multiple MS (LC-MS/MS) analysis. In our representative strain, Pseudomonas CMR12a, production of the signal molecules could be assigned to two quorum-sensing (QS) systems. The first one is the QS system for phenazine production, PhzI/PhzR, which seemed to be well conserved, since it was genetically organized in the same way as in the well-described phenazine-producing Pseudomonas strains Pseudomonas fluorescens 2-79, Pseudomonas chlororaphis PCL1391 and Pseudomonas aureofaciens 30-84. The newly characterized genes cmrI and cmrR make up the second QS system of CMR12a, under the control of the uncommon N-3-hydroxy-dodecanoyl-homoserine lactone (3-OH-C12-HSL) and with low similarity to other Pseudomonas QS systems. No clear function could yet be assigned to the CmrI/CmrR system, although it contributes to the biocontrol capability of CMR12a. Both the PhzI/PhzR and CmrI/CmrR systems are controlled by the GacS/GacA two-component regulatory system.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
PHZ OPERON, ROOT-ROT, RHIZOCTONIA-SOLANI, BIOFILM FORMATION, ANTIBIOTIC PRODUCTION, PLANT-ASSOCIATED BACTERIA, PHENAZINE-1-CARBOXYLIC ACID, FLUORESCENT PSEUDOMONAS, BIOLOGICAL-CONTROL, CHLORORAPHIS PCL1391
journal title
MICROBIOLOGY-SGM
Microbiology-(UK)
volume
157
issue
2
pages
459 - 472
Web of Science type
Article
Web of Science id
000288483200015
JCR category
MICROBIOLOGY
JCR impact factor
3.061 (2011)
JCR rank
42/111 (2011)
JCR quartile
2 (2011)
ISSN
1350-0872
DOI
10.1099/mic.0.043125-0
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1206025
handle
http://hdl.handle.net/1854/LU-1206025
date created
2011-04-11 13:24:49
date last changed
2011-05-06 09:13:42
@article{1206025,
  abstract     = {Forty fluorescent Pseudomonas strains isolated from white and red cocoyam roots were tested for their ability to synthesize N-acyl-L-homoserine lactones (acyl-HSLs). Remarkably, only isolates from the red cocoyam rhizosphere that were antagonistic against the cocoyam root rot pathogen Pythium myriotylum and synthesized phenazine antibiotics produced acyl-HSLs. This supports the assumption that acyl-HSL production is related to the antagonistic activity of the strains. After detection, the signal molecules were identified through TLC-overlay and liquid chromatography-multiple MS (LC-MS/MS) analysis. In our representative strain, Pseudomonas CMR12a, production of the signal molecules could be assigned to two quorum-sensing (QS) systems. The first one is the QS system for phenazine production, PhzI/PhzR, which seemed to be well conserved, since it was genetically organized in the same way as in the well-described phenazine-producing Pseudomonas strains Pseudomonas fluorescens 2-79, Pseudomonas chlororaphis PCL1391 and Pseudomonas aureofaciens 30-84. The newly characterized genes cmrI and cmrR make up the second QS system of CMR12a, under the control of the uncommon N-3-hydroxy-dodecanoyl-homoserine lactone (3-OH-C12-HSL) and with low similarity to other Pseudomonas QS systems. No clear function could yet be assigned to the CmrI/CmrR system, although it contributes to the biocontrol capability of CMR12a. Both the PhzI/PhzR and CmrI/CmrR systems are controlled by the GacS/GacA two-component regulatory system.},
  author       = {De Maeyer, Katrien and D'aes, Jolien and Hua, Hoang Gia Khuong and Perneel, Maaike and Vanhaecke, Lynn and Noppe, Herlinde and H{\"o}fte, Monica},
  issn         = {1350-0872},
  journal      = {MICROBIOLOGY-SGM},
  keyword      = {PHZ OPERON,ROOT-ROT,RHIZOCTONIA-SOLANI,BIOFILM FORMATION,ANTIBIOTIC PRODUCTION,PLANT-ASSOCIATED BACTERIA,PHENAZINE-1-CARBOXYLIC ACID,FLUORESCENT PSEUDOMONAS,BIOLOGICAL-CONTROL,CHLORORAPHIS PCL1391},
  language     = {eng},
  number       = {2},
  pages        = {459--472},
  title        = {N-Acylhomoserine lactone quorum-sensing signalling in antagonistic phenazine-producing Pseudomonas isolates from the red cocoyam rhizosphere},
  url          = {http://dx.doi.org/10.1099/mic.0.043125-0},
  volume       = {157},
  year         = {2011},
}

Chicago
De Maeyer, Katrien, Jolien D’aes, Hoang Gia Khuong Hua, Maaike Perneel, Lynn Vanhaecke, Herlinde Noppe, and Monica Höfte. 2011. “N-Acylhomoserine Lactone Quorum-sensing Signalling in Antagonistic Phenazine-producing Pseudomonas Isolates from the Red Cocoyam Rhizosphere.” Microbiology-sgm 157 (2): 459–472.
APA
De Maeyer, K., D’aes, J., Hua, H. G. K., Perneel, M., Vanhaecke, L., Noppe, H., & Höfte, M. (2011). N-Acylhomoserine lactone quorum-sensing signalling in antagonistic phenazine-producing Pseudomonas isolates from the red cocoyam rhizosphere. MICROBIOLOGY-SGM, 157(2), 459–472.
Vancouver
1.
De Maeyer K, D’aes J, Hua HGK, Perneel M, Vanhaecke L, Noppe H, et al. N-Acylhomoserine lactone quorum-sensing signalling in antagonistic phenazine-producing Pseudomonas isolates from the red cocoyam rhizosphere. MICROBIOLOGY-SGM. 2011;157(2):459–72.
MLA
De Maeyer, Katrien, Jolien D’aes, Hoang Gia Khuong Hua, et al. “N-Acylhomoserine Lactone Quorum-sensing Signalling in Antagonistic Phenazine-producing Pseudomonas Isolates from the Red Cocoyam Rhizosphere.” MICROBIOLOGY-SGM 157.2 (2011): 459–472. Print.