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N-Acylhomoserine lactone quorum-sensing signalling in antagonistic phenazine-producing Pseudomonas isolates from the red cocoyam rhizosphere

(2011) MICROBIOLOGY-SGM. 157(2). p.459-472
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Abstract
Forty fluorescent Pseudomonas strains isolated from white and red cocoyam roots were tested for their ability to synthesize N-acyl-L-homoserine lactones (acyl-HSLs). Remarkably, only isolates from the red cocoyam rhizosphere that were antagonistic against the cocoyam root rot pathogen Pythium myriotylum and synthesized phenazine antibiotics produced acyl-HSLs. This supports the assumption that acyl-HSL production is related to the antagonistic activity of the strains. After detection, the signal molecules were identified through TLC-overlay and liquid chromatography-multiple MS (LC-MS/MS) analysis. In our representative strain, Pseudomonas CMR12a, production of the signal molecules could be assigned to two quorum-sensing (QS) systems. The first one is the QS system for phenazine production, PhzI/PhzR, which seemed to be well conserved, since it was genetically organized in the same way as in the well-described phenazine-producing Pseudomonas strains Pseudomonas fluorescens 2-79, Pseudomonas chlororaphis PCL1391 and Pseudomonas aureofaciens 30-84. The newly characterized genes cmrI and cmrR make up the second QS system of CMR12a, under the control of the uncommon N-3-hydroxy-dodecanoyl-homoserine lactone (3-OH-C12-HSL) and with low similarity to other Pseudomonas QS systems. No clear function could yet be assigned to the CmrI/CmrR system, although it contributes to the biocontrol capability of CMR12a. Both the PhzI/PhzR and CmrI/CmrR systems are controlled by the GacS/GacA two-component regulatory system.
Keywords
PHZ OPERON, ROOT-ROT, RHIZOCTONIA-SOLANI, BIOFILM FORMATION, ANTIBIOTIC PRODUCTION, PLANT-ASSOCIATED BACTERIA, PHENAZINE-1-CARBOXYLIC ACID, FLUORESCENT PSEUDOMONAS, BIOLOGICAL-CONTROL, CHLORORAPHIS PCL1391

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Citation

Please use this url to cite or link to this publication:

Chicago
De Maeyer, Katrien, Jolien D’aes, Hoang Gia Khuong Hua, Maaike Perneel, Lynn Vanhaecke, Herlinde Noppe, and Monica Höfte. 2011. “N-Acylhomoserine Lactone Quorum-sensing Signalling in Antagonistic Phenazine-producing Pseudomonas Isolates from the Red Cocoyam Rhizosphere.” Microbiology-sgm 157 (2): 459–472.
APA
De Maeyer, K., D’aes, J., Hua, H. G. K., Perneel, M., Vanhaecke, L., Noppe, H., & Höfte, M. (2011). N-Acylhomoserine lactone quorum-sensing signalling in antagonistic phenazine-producing Pseudomonas isolates from the red cocoyam rhizosphere. MICROBIOLOGY-SGM, 157(2), 459–472.
Vancouver
1.
De Maeyer K, D’aes J, Hua HGK, Perneel M, Vanhaecke L, Noppe H, et al. N-Acylhomoserine lactone quorum-sensing signalling in antagonistic phenazine-producing Pseudomonas isolates from the red cocoyam rhizosphere. MICROBIOLOGY-SGM. 2011;157(2):459–72.
MLA
De Maeyer, Katrien, Jolien D’aes, Hoang Gia Khuong Hua, et al. “N-Acylhomoserine Lactone Quorum-sensing Signalling in Antagonistic Phenazine-producing Pseudomonas Isolates from the Red Cocoyam Rhizosphere.” MICROBIOLOGY-SGM 157.2 (2011): 459–472. Print.
@article{1206025,
  abstract     = {Forty fluorescent Pseudomonas strains isolated from white and red cocoyam roots were tested for their ability to synthesize N-acyl-L-homoserine lactones (acyl-HSLs). Remarkably, only isolates from the red cocoyam rhizosphere that were antagonistic against the cocoyam root rot pathogen Pythium myriotylum and synthesized phenazine antibiotics produced acyl-HSLs. This supports the assumption that acyl-HSL production is related to the antagonistic activity of the strains. After detection, the signal molecules were identified through TLC-overlay and liquid chromatography-multiple MS (LC-MS/MS) analysis. In our representative strain, Pseudomonas CMR12a, production of the signal molecules could be assigned to two quorum-sensing (QS) systems. The first one is the QS system for phenazine production, PhzI/PhzR, which seemed to be well conserved, since it was genetically organized in the same way as in the well-described phenazine-producing Pseudomonas strains Pseudomonas fluorescens 2-79, Pseudomonas chlororaphis PCL1391 and Pseudomonas aureofaciens 30-84. The newly characterized genes cmrI and cmrR make up the second QS system of CMR12a, under the control of the uncommon N-3-hydroxy-dodecanoyl-homoserine lactone (3-OH-C12-HSL) and with low similarity to other Pseudomonas QS systems. No clear function could yet be assigned to the CmrI/CmrR system, although it contributes to the biocontrol capability of CMR12a. Both the PhzI/PhzR and CmrI/CmrR systems are controlled by the GacS/GacA two-component regulatory system.},
  author       = {De Maeyer, Katrien and D'aes, Jolien and Hua, Hoang Gia Khuong and Perneel, Maaike and Vanhaecke, Lynn and Noppe, Herlinde and H{\"o}fte, Monica},
  issn         = {1350-0872},
  journal      = {MICROBIOLOGY-SGM},
  keyword      = {PHZ OPERON,ROOT-ROT,RHIZOCTONIA-SOLANI,BIOFILM FORMATION,ANTIBIOTIC PRODUCTION,PLANT-ASSOCIATED BACTERIA,PHENAZINE-1-CARBOXYLIC ACID,FLUORESCENT PSEUDOMONAS,BIOLOGICAL-CONTROL,CHLORORAPHIS PCL1391},
  language     = {eng},
  number       = {2},
  pages        = {459--472},
  title        = {N-Acylhomoserine lactone quorum-sensing signalling in antagonistic phenazine-producing Pseudomonas isolates from the red cocoyam rhizosphere},
  url          = {http://dx.doi.org/10.1099/mic.0.043125-0},
  volume       = {157},
  year         = {2011},
}

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