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Applicability of a yeast bioassay in the detection of steroid esters in hair

Ilse Becue UGent, Toine FH Bovee, Christof Van Poucke UGent, Maria J Groot, Michel WF Nielen and Carlos Van Peteghem UGent (2011) ANALYTICAL AND BIOANALYTICAL CHEMISTRY. 399(3). p.1031-1039
abstract
The aim of the present study was to demonstrate the applicability of a yeast androgen and estrogen bioassay in the detection of steroid esters in hair samples of animals treated with a hormone ester cocktail. The outcome of the activity screenings was critically compared with the results previously obtained with LC-MS/MS analysis. Hair samples of one pour-on treated animal, 10 ml DMSO containing 25 mg estradiol benzoate (EB), 60 mg testosterone decanoate (TD) and 60 mg testosterone cypionate (TC), were selected and analyzed with the androgen and estrogen yeast bioassay. Results showed that by the introduction of a hydrolysis step, bioassays can be used to screen for the presence of hormone esters in hair samples. Based on the difference in fluorescence responses between the non-hydrolyzed and the hydrolyzed hair samples, it was possible to detect the presence of EB up to at least 56 days after a single pour-on treatment and to detect the presence of TC and TD up to at least 14 days after the treatment. Although the LC-MS/MS analysis could detect TC and TD up to 49 days after treatment, bioassays have the advantage that they can also detect any (un) known steroid ester.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
GREEN FLUORESCENT PROTEIN, ESTROGENIC ACTIVITY, TANDEM MASS-SPECTROMETRY, BOVINE HAIR, ESTRADIOL BENZOATE, ANABOLIC-STEROIDS, CALF URINE, VALIDATION, SAMPLES, EXPRESSION, Testosterone ester, Estradiol benzoate, Yeast bioassay, Untargeted analysis, Hair
journal title
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Anal. Bioanal. Chem.
editor
Marianne Bailleul UGent
volume
399
issue
3
pages
1031 - 1039
Web of Science type
Article
Web of Science id
000286599100004
JCR category
CHEMISTRY, ANALYTICAL
JCR impact factor
3.778 (2011)
JCR rank
13/73 (2011)
JCR quartile
1 (2011)
ISSN
1618-2642
DOI
10.1007/s00216-010-4401-5
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1206012
handle
http://hdl.handle.net/1854/LU-1206012
date created
2011-04-11 13:20:33
date last changed
2015-06-17 09:31:51
@article{1206012,
  abstract     = {The aim of the present study was to demonstrate the applicability of a yeast androgen and estrogen bioassay in the detection of steroid esters in hair samples of animals treated with a hormone ester cocktail. The outcome of the activity screenings was critically compared with the results previously obtained with LC-MS/MS analysis. Hair samples of one pour-on treated animal, 10 ml DMSO containing 25 mg estradiol benzoate (EB), 60 mg testosterone decanoate (TD) and 60 mg testosterone cypionate (TC), were selected and analyzed with the androgen and estrogen yeast bioassay. Results showed that by the introduction of a hydrolysis step, bioassays can be used to screen for the presence of hormone esters in hair samples. Based on the difference in fluorescence responses between the non-hydrolyzed and the hydrolyzed hair samples, it was possible to detect the presence of EB up to at least 56 days after a single pour-on treatment and to detect the presence of TC and TD up to at least 14 days after the treatment. Although the LC-MS/MS analysis could detect TC and TD up to 49 days after treatment, bioassays have the advantage that they can also detect any (un) known steroid ester.},
  author       = {Becue, Ilse and Bovee, Toine FH and Van Poucke, Christof and Groot, Maria J and Nielen, Michel WF and Van Peteghem, Carlos},
  editor       = {Bailleul, Marianne},
  issn         = {1618-2642},
  journal      = {ANALYTICAL AND BIOANALYTICAL CHEMISTRY},
  keyword      = {GREEN FLUORESCENT PROTEIN,ESTROGENIC ACTIVITY,TANDEM MASS-SPECTROMETRY,BOVINE HAIR,ESTRADIOL BENZOATE,ANABOLIC-STEROIDS,CALF URINE,VALIDATION,SAMPLES,EXPRESSION,Testosterone ester,Estradiol benzoate,Yeast bioassay,Untargeted analysis,Hair},
  language     = {eng},
  number       = {3},
  pages        = {1031--1039},
  title        = {Applicability of a yeast bioassay in the detection of steroid esters in hair},
  url          = {http://dx.doi.org/10.1007/s00216-010-4401-5},
  volume       = {399},
  year         = {2011},
}

Chicago
Becue, Ilse, Toine FH Bovee, Christof Van Poucke, Maria J Groot, Michel WF Nielen, and Carlos Van Peteghem. 2011. “Applicability of a Yeast Bioassay in the Detection of Steroid Esters in Hair.” Ed. Marianne Bailleul. Analytical and Bioanalytical Chemistry 399 (3): 1031–1039.
APA
Becue, I., Bovee, T. F., Van Poucke, C., Groot, M. J., Nielen, M. W., & Van Peteghem, C. (2011). Applicability of a yeast bioassay in the detection of steroid esters in hair. (M. Bailleul, Ed.)ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 399(3), 1031–1039.
Vancouver
1.
Becue I, Bovee TF, Van Poucke C, Groot MJ, Nielen MW, Van Peteghem C. Applicability of a yeast bioassay in the detection of steroid esters in hair. Bailleul M, editor. ANALYTICAL AND BIOANALYTICAL CHEMISTRY. 2011;399(3):1031–9.
MLA
Becue, Ilse, Toine FH Bovee, Christof Van Poucke, et al. “Applicability of a Yeast Bioassay in the Detection of Steroid Esters in Hair.” Ed. Marianne Bailleul. ANALYTICAL AND BIOANALYTICAL CHEMISTRY 399.3 (2011): 1031–1039. Print.