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Arcobacter population dynamics in pigs on farrow-to-finish farms

Sarah De Smet UGent, Lieven De Zutter UGent, Lies Debruyne UGent, Frederic Vangroenweghe, Peter Vandamme UGent and Kurt Houf UGent (2011) APPLIED AND ENVIRONMENTAL MICROBIOLOGY. 77(5). p.1732-1738
abstract
Healthy pigs are an important reservoir for the emerging human pathogen Arcobacter which can result in contamination of porcine carcasses and pork and the spread of arcobacters into the environment. Up to now, the excretion of arcobacters by pigs has been studied, but information about the transmission routes in fattening pigs is lacking. The present study aimed to elucidate the Arcobacter population dynamics in pigs during the fattening period on four farrow-to-finish farms. On each farm, 30 clinically healthy, 12-week-old piglets were selected. Fecal samples were collected on 10 sampling occasions until a slaughter age of 30 weeks was reached. Arcobacter spp. were isolated by a selective method and identified by multiplex PCR. The genetic diversity was examined by amplified fragment length polymorphism and enterobacterial repetitive intergenic consensus PCR. The Arcobacter presence in the fecal samples on the four farms ranged from 11.3 to 50.0%, with excretion levels of up to 10(4) CFU/g feces. The ratio in which Arcobacter species were isolated varied between the farms and over time. Characterization revealed a high degree of genotypic diversity among the isolates. Arcobacter strains persisted and spread within the finishing unit during the fattening period. The occurrence of both unique and shared genotypes in pigs in adjacent and nonadjacent pens demonstrates that transmission routes other than fecal-oral transmission occur.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
PATHOGEN ARCOBACTER, FATTENING PIGS, SP NOV., CAMPYLOBACTER-JEJUNI, PREVALENCE, BUTZLERI, RETAIL, SPP., CONTAMINATION, COLONIZATION
journal title
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Appl. Environ. Microbiol.
volume
77
issue
5
pages
1732 - 1738
Web of Science type
Article
Web of Science id
000287700100025
JCR category
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
JCR impact factor
3.829 (2011)
JCR rank
29/157 (2011)
JCR quartile
1 (2011)
ISSN
0099-2240
DOI
10.1128/AEM.02409-10
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1203935
handle
http://hdl.handle.net/1854/LU-1203935
date created
2011-04-06 17:19:56
date last changed
2011-04-11 15:53:00
@article{1203935,
  abstract     = {Healthy pigs are an important reservoir for the emerging human pathogen Arcobacter which can result in contamination of porcine carcasses and pork and the spread of arcobacters into the environment. Up to now, the excretion of arcobacters by pigs has been studied, but information about the transmission routes in fattening pigs is lacking. The present study aimed to elucidate the Arcobacter population dynamics in pigs during the fattening period on four farrow-to-finish farms. On each farm, 30 clinically healthy, 12-week-old piglets were selected. Fecal samples were collected on 10 sampling occasions until a slaughter age of 30 weeks was reached. Arcobacter spp. were isolated by a selective method and identified by multiplex PCR. The genetic diversity was examined by amplified fragment length polymorphism and enterobacterial repetitive intergenic consensus PCR. The Arcobacter presence in the fecal samples on the four farms ranged from 11.3 to 50.0\%, with excretion levels of up to 10(4) CFU/g feces. The ratio in which Arcobacter species were isolated varied between the farms and over time. Characterization revealed a high degree of genotypic diversity among the isolates. Arcobacter strains persisted and spread within the finishing unit during the fattening period. The occurrence of both unique and shared genotypes in pigs in adjacent and nonadjacent pens demonstrates that transmission routes other than fecal-oral transmission occur.},
  author       = {De Smet, Sarah and De Zutter, Lieven and Debruyne, Lies and Vangroenweghe, Frederic and Vandamme, Peter and Houf, Kurt},
  issn         = {0099-2240},
  journal      = {APPLIED AND ENVIRONMENTAL MICROBIOLOGY},
  keyword      = {PATHOGEN ARCOBACTER,FATTENING PIGS,SP NOV.,CAMPYLOBACTER-JEJUNI,PREVALENCE,BUTZLERI,RETAIL,SPP.,CONTAMINATION,COLONIZATION},
  language     = {eng},
  number       = {5},
  pages        = {1732--1738},
  title        = {Arcobacter population dynamics in pigs on farrow-to-finish farms},
  url          = {http://dx.doi.org/10.1128/AEM.02409-10},
  volume       = {77},
  year         = {2011},
}

Chicago
De Smet, Sarah, Lieven De Zutter, Lies Debruyne, Frederic Vangroenweghe, Peter Vandamme, and Kurt Houf. 2011. “Arcobacter Population Dynamics in Pigs on Farrow-to-finish Farms.” Applied and Environmental Microbiology 77 (5): 1732–1738.
APA
De Smet, Sarah, De Zutter, L., Debruyne, L., Vangroenweghe, F., Vandamme, P., & Houf, K. (2011). Arcobacter population dynamics in pigs on farrow-to-finish farms. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 77(5), 1732–1738.
Vancouver
1.
De Smet S, De Zutter L, Debruyne L, Vangroenweghe F, Vandamme P, Houf K. Arcobacter population dynamics in pigs on farrow-to-finish farms. APPLIED AND ENVIRONMENTAL MICROBIOLOGY. 2011;77(5):1732–8.
MLA
De Smet, Sarah, Lieven De Zutter, Lies Debruyne, et al. “Arcobacter Population Dynamics in Pigs on Farrow-to-finish Farms.” APPLIED AND ENVIRONMENTAL MICROBIOLOGY 77.5 (2011): 1732–1738. Print.