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Hafnia paralvei sp nov., formerly known as Hafnia alvei hybridization group 2

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Abstract
It has been shown previously, based largely on DNA-DNA hybridizations and partial 16S rRNA gene sequencing, that Hafnia alvei is genotypically heterogeneous and consists of at least two DNA hybridization groups (HGs). In the present study, the taxonomic status of H. alvei HGs 1 and 2 was reassessed. A panel of 24 reference strains and isolates previously assigned to one of the two HGs in H. alvei was subjected to (GTG)(5)-PCR fingerprinting; this resulted in the delineation of two (GTG)(5)-PCR clusters in perfect accordance with the respective HG designations. Based on full 16S rRNA gene sequencing of a selection of reference strains, H. alvei HGs 1 and 2 showed internal sequence similarities of 99.8 and 99.5%, respectively. Between the two groups, sequence similarities ranged from 98.8 to 99.1%. Mean DNA-DNA hybridization values of 74.7-99.9% were obtained within each of the two HGs, whereas cross-hybridizations between members of H. alvei HG 1 (including ATCC 133371) and HG 2 revealed only 32.7-48.7% DNA-DNA hybridization. Previously published and new phenotypic data revealed that a combination of malonate assimilation and beta-glucosidase activity enabled correct assignment of Hafnia isolates to one of the two HGs. Collectively, taxonomic data from this study confirm that H. alvei comprises at least two taxa at the species level, of which HG 1 corresponds to H. alvei sensu stricto because it includes the type strain ATCC 13337(T). Strains formerly classified as members of H. alvei HG 2 represent a novel species, for which the name Hafnia paralvei sp. nov. is proposed; ATCC 29927(T) (=CDC 4510-73(T) =LMG 24706(T)), the former reference strain of H. alvei HG 2, is designated the type strain.
Keywords
DEOXYRIBONUCLEIC-ACID, IDENTIFICATION, GENUS HAFNIA, DNA

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Chicago
Huys, Geert, Margo Cnockaert, Sharon L Abbott, J Michael Janda, and Peter Vandamme. 2010. “Hafnia Paralvei Sp Nov., Formerly Known as Hafnia Alvei Hybridization Group 2.” International Journal of Systematic and Evolutionary Microbiology 60 (8): 1725–1728.
APA
Huys, G., Cnockaert, M., Abbott, S. L., Janda, J. M., & Vandamme, P. (2010). Hafnia paralvei sp nov., formerly known as Hafnia alvei hybridization group 2. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 60(8), 1725–1728.
Vancouver
1.
Huys G, Cnockaert M, Abbott SL, Janda JM, Vandamme P. Hafnia paralvei sp nov., formerly known as Hafnia alvei hybridization group 2. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY. 2010;60(8):1725–8.
MLA
Huys, Geert, Margo Cnockaert, Sharon L Abbott, et al. “Hafnia Paralvei Sp Nov., Formerly Known as Hafnia Alvei Hybridization Group 2.” INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 60.8 (2010): 1725–1728. Print.
@article{1203775,
  abstract     = {It has been shown previously, based largely on DNA-DNA hybridizations and partial 16S rRNA gene sequencing, that Hafnia alvei is genotypically heterogeneous and consists of at least two DNA hybridization groups (HGs). In the present study, the taxonomic status of H. alvei HGs 1 and 2 was reassessed. A panel of 24 reference strains and isolates previously assigned to one of the two HGs in H. alvei was subjected to (GTG)(5)-PCR fingerprinting; this resulted in the delineation of two (GTG)(5)-PCR clusters in perfect accordance with the respective HG designations. Based on full 16S rRNA gene sequencing of a selection of reference strains, H. alvei HGs 1 and 2 showed internal sequence similarities of 99.8 and 99.5%, respectively. Between the two groups, sequence similarities ranged from 98.8 to 99.1%. Mean DNA-DNA hybridization values of 74.7-99.9% were obtained within each of the two HGs, whereas cross-hybridizations between members of H. alvei HG 1 (including ATCC 133371) and HG 2 revealed only 32.7-48.7% DNA-DNA hybridization. Previously published and new phenotypic data revealed that a combination of malonate assimilation and beta-glucosidase activity enabled correct assignment of Hafnia isolates to one of the two HGs. Collectively, taxonomic data from this study confirm that H. alvei comprises at least two taxa at the species level, of which HG 1 corresponds to H. alvei sensu stricto because it includes the type strain ATCC 13337(T). Strains formerly classified as members of H. alvei HG 2 represent a novel species, for which the name Hafnia paralvei sp. nov. is proposed; ATCC 29927(T) (=CDC 4510-73(T) =LMG 24706(T)), the former reference strain of H. alvei HG 2, is designated the type strain.},
  author       = {Huys, Geert and Cnockaert, Margo and Abbott, Sharon L and Janda, J Michael and Vandamme, Peter},
  issn         = {1466-5026},
  journal      = {INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY},
  keywords     = {DEOXYRIBONUCLEIC-ACID,IDENTIFICATION,GENUS HAFNIA,DNA},
  language     = {eng},
  number       = {8},
  pages        = {1725--1728},
  title        = {Hafnia paralvei sp nov., formerly known as Hafnia alvei hybridization group 2},
  url          = {http://dx.doi.org/10.1099/ijs.0.018606-0},
  volume       = {60},
  year         = {2010},
}

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