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N-linked glycosylation of folded proteins by the bacterial oligosaccharyltransferase

Michael Kowarik, Shin Numao, Mario F Feldman, Benjamin L Schulz, Nico Callewaert UGent, Eva Kiermaier, Ina Catrein and Markus Aebi (2006) SCIENCE. 314(5802). p.1148-1150
abstract
N-linked protein glycosylation is found in all domains of life. In eukaryotes, it is the most abundant protein modification of secretory and membrane proteins, and the process is coupled to protein translocation and folding. We found that in bacteria, N-glycosylation can occur independently of the protein translocation machinery. In an in vitro assay, bacterial oligosaccharyltransferase glycosylated a folded endogenous substrate protein with high efficiency and folded bovine ribonuclease A with low efficiency. Unfolding the eukaryotic substrate greatly increased glycosylation. We propose that in the bacterial system, glycosylation sites are located in flexible parts of folded proteins, whereas the eukaryotic cotranslational glycosylation evolved to a mechanism presenting the substrate in a flexible form before folding.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
CAMPYLOBACTER-JEJUNI, TAT PATHWAY, ESCHERICHIA-COLI, TRANSLOCATION, COMPLEX, ENDOPLASMIC-RETICULUM, GREEN FLUORESCENT PROTEIN, TRANSFERASE, SPECIFICITY, RECOGNITION
journal title
SCIENCE
Science
volume
314
issue
5802
pages
1148 - 1150
Web of Science type
Article
Web of Science id
000242045800045
JCR category
MULTIDISCIPLINARY SCIENCES
JCR impact factor
30.028 (2006)
JCR rank
1/50 (2006)
JCR quartile
1 (2006)
ISSN
0036-8075
DOI
10.1126/science.1134351
language
English
UGent publication?
no
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1203449
handle
http://hdl.handle.net/1854/LU-1203449
date created
2011-04-06 12:29:19
date last changed
2016-12-19 15:45:14
@article{1203449,
  abstract     = {N-linked protein glycosylation is found in all domains of life. In eukaryotes, it is the most abundant protein modification of secretory and membrane proteins, and the process is coupled to protein translocation and folding. We found that in bacteria, N-glycosylation can occur independently of the protein translocation machinery. In an in vitro assay, bacterial oligosaccharyltransferase glycosylated a folded endogenous substrate protein with high efficiency and folded bovine ribonuclease A with low efficiency. Unfolding the eukaryotic substrate greatly increased glycosylation. We propose that in the bacterial system, glycosylation sites are located in flexible parts of folded proteins, whereas the eukaryotic cotranslational glycosylation evolved to a mechanism presenting the substrate in a flexible form before folding.},
  author       = {Kowarik, Michael and Numao, Shin and Feldman, Mario F and Schulz, Benjamin L and Callewaert, Nico and Kiermaier, Eva and Catrein, Ina and Aebi, Markus},
  issn         = {0036-8075},
  journal      = {SCIENCE},
  keyword      = {CAMPYLOBACTER-JEJUNI,TAT PATHWAY,ESCHERICHIA-COLI,TRANSLOCATION,COMPLEX,ENDOPLASMIC-RETICULUM,GREEN FLUORESCENT PROTEIN,TRANSFERASE,SPECIFICITY,RECOGNITION},
  language     = {eng},
  number       = {5802},
  pages        = {1148--1150},
  title        = {N-linked glycosylation of folded proteins by the bacterial oligosaccharyltransferase},
  url          = {http://dx.doi.org/10.1126/science.1134351},
  volume       = {314},
  year         = {2006},
}

Chicago
Kowarik, Michael, Shin Numao, Mario F Feldman, Benjamin L Schulz, Nico Callewaert, Eva Kiermaier, Ina Catrein, and Markus Aebi. 2006. “N-linked Glycosylation of Folded Proteins by the Bacterial Oligosaccharyltransferase.” Science 314 (5802): 1148–1150.
APA
Kowarik, M., Numao, S., Feldman, M. F., Schulz, B. L., Callewaert, N., Kiermaier, E., Catrein, I., et al. (2006). N-linked glycosylation of folded proteins by the bacterial oligosaccharyltransferase. SCIENCE, 314(5802), 1148–1150.
Vancouver
1.
Kowarik M, Numao S, Feldman MF, Schulz BL, Callewaert N, Kiermaier E, et al. N-linked glycosylation of folded proteins by the bacterial oligosaccharyltransferase. SCIENCE. 2006;314(5802):1148–50.
MLA
Kowarik, Michael, Shin Numao, Mario F Feldman, et al. “N-linked Glycosylation of Folded Proteins by the Bacterial Oligosaccharyltransferase.” SCIENCE 314.5802 (2006): 1148–1150. Print.