Advanced search
1 file | 677.26 KB

O mannosylation of α-dystroglycan is essential for lymphocytic choriomeningitis virus receptor function

(2005) JOURNAL OF VIROLOGY. 79(22). p.14297-14308
Author
Organization
Abstract
alpha-Dystroglycan (alpha-DG) was identified as a common receptor for lymphocytic choriomeningitis virus (LCMW) and several other arenaviruses including the human pathogenic Lassa fever virus. Initial work postulated that interactions between arenavirus glycoproteins and alpha-DG are based on protein-protein interactions. We found, however, that susceptibility toward LCMV infection differed in various cell lines despite them expressing comparable levels of DG, suggesting that posttranslational modifications of alpha-DG would be involved in viral receptor function. Here, we demonstrate that glycosylation of alpha-DG, and in particular, 0 mannosylation, which is a rare type of O-linked glycosylation in mammals, is essential for LCMV receptor function. Cells that are defective in components of the O-mannosylation pathway showed strikingly reduced LCMV infectibility. As defective O mannosylation is associated with severe clinical symptoms in mammals such as congenital muscular dystrophies, it is likely that LCMV and potentially other arenaviruses may have selected this conserved and crucial posttranslational modification as the primary target structure for cell entry and infection.
Keywords
PROTEIN, GLYCOSYLATION

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 677.26 KB

Citation

Please use this url to cite or link to this publication:

Chicago
Imperiali, Mauro, Claudio Thoma, Ernesto Pavoni, Andrea Brancaccio, Nico Callewaert, and Annette Oxenius. 2005. “O Mannosylation of Α-dystroglycan Is Essential for Lymphocytic Choriomeningitis Virus Receptor Function.” Journal of Virology 79 (22): 14297–14308.
APA
Imperiali, M., Thoma, C., Pavoni, E., Brancaccio, A., Callewaert, N., & Oxenius, A. (2005). O mannosylation of α-dystroglycan is essential for lymphocytic choriomeningitis virus receptor function. JOURNAL OF VIROLOGY, 79(22), 14297–14308.
Vancouver
1.
Imperiali M, Thoma C, Pavoni E, Brancaccio A, Callewaert N, Oxenius A. O mannosylation of α-dystroglycan is essential for lymphocytic choriomeningitis virus receptor function. JOURNAL OF VIROLOGY. 2005;79(22):14297–308.
MLA
Imperiali, Mauro, Claudio Thoma, Ernesto Pavoni, et al. “O Mannosylation of Α-dystroglycan Is Essential for Lymphocytic Choriomeningitis Virus Receptor Function.” JOURNAL OF VIROLOGY 79.22 (2005): 14297–14308. Print.
@article{1203275,
  abstract     = {alpha-Dystroglycan (alpha-DG) was identified as a common receptor for lymphocytic choriomeningitis virus (LCMW) and several other arenaviruses including the human pathogenic Lassa fever virus. Initial work postulated that interactions between arenavirus glycoproteins and alpha-DG are based on protein-protein interactions. We found, however, that susceptibility toward LCMV infection differed in various cell lines despite them expressing comparable levels of DG, suggesting that posttranslational modifications of alpha-DG would be involved in viral receptor function. Here, we demonstrate that glycosylation of alpha-DG, and in particular, 0 mannosylation, which is a rare type of O-linked glycosylation in mammals, is essential for LCMV receptor function. Cells that are defective in components of the O-mannosylation pathway showed strikingly reduced LCMV infectibility. As defective O mannosylation is associated with severe clinical symptoms in mammals such as congenital muscular dystrophies, it is likely that LCMV and potentially other arenaviruses may have selected this conserved and crucial posttranslational modification as the primary target structure for cell entry and infection.},
  author       = {Imperiali, Mauro and Thoma, Claudio and Pavoni, Ernesto and Brancaccio, Andrea and Callewaert, Nico and Oxenius, Annette},
  issn         = {0022-538X},
  journal      = {JOURNAL OF VIROLOGY},
  keywords     = {PROTEIN,GLYCOSYLATION},
  language     = {eng},
  number       = {22},
  pages        = {14297--14308},
  title        = {O mannosylation of α-dystroglycan is essential for lymphocytic choriomeningitis virus receptor function},
  url          = {http://dx.doi.org/10.1128/JVI.79.22.14297-14308.2005},
  volume       = {79},
  year         = {2005},
}

Altmetric
View in Altmetric
Web of Science
Times cited: