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Identification of a Streptococcus pneumoniae gene locus encoding proteins of an ABC phosphate transporter and a two-component regulatory system

R Novak, Anje Cauwels UGent, E Charpentier and E Tuomanen (1999) JOURNAL OF BACTERIOLOGY. 181(4). p.1126-1133
abstract
The Escherichia coli Pst system belongs to the family of ABC transporters, It is part of a phosphate (PHO) regulon which is regulated by extracellular phosphate, Under conditions of phosphate limitation, the response regulator PhoB is phosphorylated by the histidine kinase PhoR and binds to promoters that share a consensus PHO box, Under conditions of phosphate excess, PhoR, Pst, and PhoU downregulate the PHO regulon. Screening of a library of pneumococcal mutants with defects in exported proteins revealed a putative two-component regulatory system, PnpR-PnpS, and a downstream ABC transporter, similar to the Pst system in E. coli including a gene encoding a PhoU protein. Similar to E, coli, mutagenesis of the ATP-binding cassette gene, pstB, resulted in decreased uptake of phosphate, The effects of the loss of the pneumococcal Pst system extended to decreased transformation and lysis, Withdrawal of phosphate led to transformation deficiency in the parent strain R6x hut not to penicillin tolerance, suggesting that reduced bacterial death was independent of phosphate, None of these phenotypes was observed in the pneumococcal loss-of-function mutant phoU, By using a lacZ reporter construct, it was demonstrated that expression of the two-component regulatory system PnpR-PnpS was not influenced by different concentrations of phosphate, These results suggest a more complex role of the Pst system in pneumococcal physiology than in that of E, coli.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
COMPETENCE-INDUCED OPERON, SIGNAL-TRANSDUCTION, BACILLUS-SUBTILIS, ESCHERICHIA-COLI, NUCLEOTIDE-SEQUENCE, DNA FRAGMENTS, TRANSFORMATION, BACTERIA, REGULON, RESISTANCE
journal title
JOURNAL OF BACTERIOLOGY
J. Bacteriol.
volume
181
issue
4
pages
1126 - 1133
Web of Science type
Article
Web of Science id
000078602700008
ISSN
0021-9193
language
English
UGent publication?
no
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1203049
handle
http://hdl.handle.net/1854/LU-1203049
date created
2011-04-05 16:55:57
date last changed
2016-12-19 15:45:49
@article{1203049,
  abstract     = {The Escherichia coli Pst system belongs to the family of ABC transporters, It is part of a phosphate (PHO) regulon which is regulated by extracellular phosphate, Under conditions of phosphate limitation, the response regulator PhoB is phosphorylated by the histidine kinase PhoR and binds to promoters that share a consensus PHO box, Under conditions of phosphate excess, PhoR, Pst, and PhoU downregulate the PHO regulon. Screening of a library of pneumococcal mutants with defects in exported proteins revealed a putative two-component regulatory system, PnpR-PnpS, and a downstream ABC transporter, similar to the Pst system in E. coli including a gene encoding a PhoU protein. Similar to E, coli, mutagenesis of the ATP-binding cassette gene, pstB, resulted in decreased uptake of phosphate, The effects of the loss of the pneumococcal Pst system extended to decreased transformation and lysis, Withdrawal of phosphate led to transformation deficiency in the parent strain R6x hut not to penicillin tolerance, suggesting that reduced bacterial death was independent of phosphate, None of these phenotypes was observed in the pneumococcal loss-of-function mutant phoU, By using a lacZ reporter construct, it was demonstrated that expression of the two-component regulatory system PnpR-PnpS was not influenced by different concentrations of phosphate, These results suggest a more complex role of the Pst system in pneumococcal physiology than in that of E, coli.},
  author       = {Novak, R and Cauwels, Anje and Charpentier, E and Tuomanen, E},
  issn         = {0021-9193},
  journal      = {JOURNAL OF BACTERIOLOGY},
  keyword      = {COMPETENCE-INDUCED OPERON,SIGNAL-TRANSDUCTION,BACILLUS-SUBTILIS,ESCHERICHIA-COLI,NUCLEOTIDE-SEQUENCE,DNA FRAGMENTS,TRANSFORMATION,BACTERIA,REGULON,RESISTANCE},
  language     = {eng},
  number       = {4},
  pages        = {1126--1133},
  title        = {Identification of a Streptococcus pneumoniae gene locus encoding proteins of an ABC phosphate transporter and a two-component regulatory system},
  volume       = {181},
  year         = {1999},
}

Chicago
Novak, R, Anje Cauwels, E Charpentier, and E Tuomanen. 1999. “Identification of a Streptococcus Pneumoniae Gene Locus Encoding Proteins of an ABC Phosphate Transporter and a Two-component Regulatory System.” Journal of Bacteriology 181 (4): 1126–1133.
APA
Novak, R., Cauwels, A., Charpentier, E., & Tuomanen, E. (1999). Identification of a Streptococcus pneumoniae gene locus encoding proteins of an ABC phosphate transporter and a two-component regulatory system. JOURNAL OF BACTERIOLOGY, 181(4), 1126–1133.
Vancouver
1.
Novak R, Cauwels A, Charpentier E, Tuomanen E. Identification of a Streptococcus pneumoniae gene locus encoding proteins of an ABC phosphate transporter and a two-component regulatory system. JOURNAL OF BACTERIOLOGY. 1999;181(4):1126–33.
MLA
Novak, R, Anje Cauwels, E Charpentier, et al. “Identification of a Streptococcus Pneumoniae Gene Locus Encoding Proteins of an ABC Phosphate Transporter and a Two-component Regulatory System.” JOURNAL OF BACTERIOLOGY 181.4 (1999): 1126–1133. Print.