Ghent University Academic Bibliography

Advanced

Coexistence of CD14-dependent and independent pathways for stimulation of human monocytes by gram-positive bacteria

Anje Cauwels UGent, E Wan, M Leismann and E Tuomanen (1997) INFECTION AND IMMUNITY. 65(8). p.3255-3260
abstract
The cell wall is a key inflammatory agent of gram-positive bacteria, Possible receptors mediating cell wall-induced inflammation include CD14 and platelet-activating factor (PAF) receptor, To delineate the conditions under which these various receptors might be used, human monocytic THP-I cells and heparinized whole human blood were stimulated with lipopolysaccharide (LPS), intact Streptococcus pneumoniae bacteria, or purified pneumococcal cell wall. THP-1 culture supernatant or cell-free plasma was analyzed for the presence of tumor necrosis factor, interleukin-1 beta (IL-1 beta), and IL-6. For the cultured monocytes, anti-CD14 inhibited induction of the inflammatory cytokines by the cell wall and LPS but not by intact pneumococcal bacteria, Despite the difference in CD-14 usage, the intracellular pathways induced by the three agents demonstrated similarities, as revealed in the presence of specific signal transduction inhibitors such as cholera toxin, pertussis toxin, and genistein, Cytokine production in whole human blood indicated that anti-CD14 failed to block responses to cell wall and intact pneumococci, whereas while LPS-induced responses were inhibited, PAF receptor antagonist had no effect under any conditions in both assays, These results indicate that although cell walls bind to both CD14 and PAF receptor, only CD14 appears to engender a cytokine response under restricted conditions, Furthermore, host cell responses to intact pneumococci are consistently independent of CD14 and PAF receptor.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
LIPOPOLYSACCHARIDE-BINDING-PROTEIN, PLATELET-ACTIVATING-FACTOR, TUMOR-NECROSIS-FACTOR, STREPTOCOCCUS-PNEUMONIAE, RECEPTOR, CD14, CELLS, INFLAMMATION, COMPONENTS, RELEASE
journal title
INFECTION AND IMMUNITY
Infect. Immun.
volume
65
issue
8
pages
3255 - 3260
Web of Science type
Article
ISSN
0019-9567
language
English
UGent publication?
no
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1203036
handle
http://hdl.handle.net/1854/LU-1203036
date created
2011-04-05 16:55:44
date last changed
2016-12-19 15:47:22
@article{1203036,
  abstract     = {The cell wall is a key inflammatory agent of gram-positive bacteria, Possible receptors mediating cell wall-induced inflammation include CD14 and platelet-activating factor (PAF) receptor, To delineate the conditions under which these various receptors might be used, human monocytic THP-I cells and heparinized whole human blood were stimulated with lipopolysaccharide (LPS), intact Streptococcus pneumoniae bacteria, or purified pneumococcal cell wall. THP-1 culture supernatant or cell-free plasma was analyzed for the presence of tumor necrosis factor, interleukin-1 beta (IL-1 beta), and IL-6. For the cultured monocytes, anti-CD14 inhibited induction of the inflammatory cytokines by the cell wall and LPS but not by intact pneumococcal bacteria, Despite the difference in CD-14 usage, the intracellular pathways induced by the three agents demonstrated similarities, as revealed in the presence of specific signal transduction inhibitors such as cholera toxin, pertussis toxin, and genistein, Cytokine production in whole human blood indicated that anti-CD14 failed to block responses to cell wall and intact pneumococci, whereas while LPS-induced responses were inhibited, PAF receptor antagonist had no effect under any conditions in both assays, These results indicate that although cell walls bind to both CD14 and PAF receptor, only CD14 appears to engender a cytokine response under restricted conditions, Furthermore, host cell responses to intact pneumococci are consistently independent of CD14 and PAF receptor.},
  author       = {Cauwels, Anje and Wan, E and Leismann, M and Tuomanen, E},
  issn         = {0019-9567},
  journal      = {INFECTION AND IMMUNITY},
  keyword      = {LIPOPOLYSACCHARIDE-BINDING-PROTEIN,PLATELET-ACTIVATING-FACTOR,TUMOR-NECROSIS-FACTOR,STREPTOCOCCUS-PNEUMONIAE,RECEPTOR,CD14,CELLS,INFLAMMATION,COMPONENTS,RELEASE},
  language     = {eng},
  number       = {8},
  pages        = {3255--3260},
  title        = {Coexistence of CD14-dependent and independent pathways for stimulation of human monocytes by gram-positive bacteria},
  volume       = {65},
  year         = {1997},
}

Chicago
Cauwels, Anje, E Wan, M Leismann, and E Tuomanen. 1997. “Coexistence of CD14-dependent and Independent Pathways for Stimulation of Human Monocytes by Gram-positive Bacteria.” Infection and Immunity 65 (8): 3255–3260.
APA
Cauwels, A., Wan, E., Leismann, M., & Tuomanen, E. (1997). Coexistence of CD14-dependent and independent pathways for stimulation of human monocytes by gram-positive bacteria. INFECTION AND IMMUNITY, 65(8), 3255–3260.
Vancouver
1.
Cauwels A, Wan E, Leismann M, Tuomanen E. Coexistence of CD14-dependent and independent pathways for stimulation of human monocytes by gram-positive bacteria. INFECTION AND IMMUNITY. 1997;65(8):3255–60.
MLA
Cauwels, Anje, E Wan, M Leismann, et al. “Coexistence of CD14-dependent and Independent Pathways for Stimulation of Human Monocytes by Gram-positive Bacteria.” INFECTION AND IMMUNITY 65.8 (1997): 3255–3260. Print.