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Presence and dynamic redistribution of type I inositol 1,4,5-trisphosphate receptors in human oocytes and embryos during in-vitro maturation, fertilization and early cleavage divisions

Pravin T Goud, Anuradha P Goud, Patric Van Oostveldt UGent and Marc Dhont (1999) MOLECULAR HUMAN REPRODUCTION. 5(5). p.441-451
abstract
We studied the presence and distribution of the intracellular calcium channel regulating type I inositol 1,4,5-trisphosphate receptors (IP3R) in human immature and mature oocytes, pronuclear zygotes and cleaved embryos using a specific antibody. Two approaches were used: (i) fluorescence immunocytochemistry using a confocal laser scanning microscope (CLSM) and (ii) Western blotting. With confocal microscopy, the receptors were found in the oocytes, fertilized zygotes as well as cleaved embryos at all stages studied. The pattern and distribution of the receptor staining in the oocytes changed gradually from a diffuse granular patchy one at the germinal vesicle (GV) stage to a reticular and predominantly peripheral one through the metaphase and metaphase II (MII) stages. After fertilization, the distribution changed gradually to both, peripheral and central in the zygotes and early 2-4-cell embryos and predominantly perinuclear in the 6-8-cell embryos. Furthermore, an overall increase in the staining intensity was observed from GV to MII stage oocytes and from zygotes to 6-8-cell embryos. We also studied the spatial distribution of the receptor in detail by constructing three-dimensional images from the serial optical sections obtained on the CLSM. Peculiar peripheral aggregates of receptor clusters were noted in the MII stage oocytes, zygotes and some blastomeres from early cleaved embryos. Finally, Western blots performed on the extracts of 72 in-vitro matured oocytes and 50 spare cleavage stage embryos showed positive bands at similar to 260 kDa. These findings coincide with and thus possibly represent the dynamic changes occurring in the cellular Ca2+ release systems through oocyte maturation, fertilization and early embryogenesis. Thus, type I IP3R are likely to play a role during these stages of early development in the human
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
INTRACELLULAR FREE CALCIUM, inositol trisphosphate, INTRACYTOPLASMIC SPERM INJECTION, calcium channels, embryogenesis, MEIOTIC MATURATION, ENDOPLASMIC-RETICULUM, immunohistochemistry, DEVELOPMENTAL-CHANGES, XENOPUS-LAEVIS, MOUSE OOCYTE, RYANODINE RECEPTORS, EGG ACTIVATION, HAMSTER OOCYTES, oocytes
journal title
MOLECULAR HUMAN REPRODUCTION
Mol. Hum. Reprod.
volume
5
issue
5
pages
441 - 451
Web of Science type
Article
Web of Science id
000080125000008
ISSN
1360-9947
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
120269
handle
http://hdl.handle.net/1854/LU-120269
alternative location
http://molehr.oxfordjournals.org/cgi/content/abstract/5/5/441
date created
2004-01-14 13:35:00
date last changed
2016-12-19 15:37:25
@article{120269,
  abstract     = {We studied the presence and distribution of the intracellular calcium channel regulating type I inositol 1,4,5-trisphosphate receptors (IP3R) in human immature and mature oocytes, pronuclear zygotes and cleaved embryos using a specific antibody. Two approaches were used: (i) fluorescence immunocytochemistry using a confocal laser scanning microscope (CLSM) and (ii) Western blotting. With confocal microscopy, the receptors were found in the oocytes, fertilized zygotes as well as cleaved embryos at all stages studied. The pattern and distribution of the receptor staining in the oocytes changed gradually from a diffuse granular patchy one at the germinal vesicle (GV) stage to a reticular and predominantly peripheral one through the metaphase and metaphase II (MII) stages. After fertilization, the distribution changed gradually to both, peripheral and central in the zygotes and early 2-4-cell embryos and predominantly perinuclear in the 6-8-cell embryos. Furthermore, an overall increase in the staining intensity was observed from GV to MII stage oocytes and from zygotes to 6-8-cell embryos. We also studied the spatial distribution of the receptor in detail by constructing three-dimensional images from the serial optical sections obtained on the CLSM. Peculiar peripheral aggregates of receptor clusters were noted in the MII stage oocytes, zygotes and some blastomeres from early cleaved embryos. Finally, Western blots performed on the extracts of 72 in-vitro matured oocytes and 50 spare cleavage stage embryos showed positive bands at similar to 260 kDa. These findings coincide with and thus possibly represent the dynamic changes occurring in the cellular Ca2+ release systems through oocyte maturation, fertilization and early embryogenesis. Thus, type I IP3R are likely to play a role during these stages of early development in the human},
  author       = {Goud, Pravin T and Goud, Anuradha P and Van Oostveldt, Patric and Dhont, Marc},
  issn         = {1360-9947},
  journal      = {MOLECULAR HUMAN REPRODUCTION},
  keyword      = {INTRACELLULAR FREE CALCIUM,inositol trisphosphate,INTRACYTOPLASMIC SPERM INJECTION,calcium channels,embryogenesis,MEIOTIC MATURATION,ENDOPLASMIC-RETICULUM,immunohistochemistry,DEVELOPMENTAL-CHANGES,XENOPUS-LAEVIS,MOUSE OOCYTE,RYANODINE RECEPTORS,EGG ACTIVATION,HAMSTER OOCYTES,oocytes},
  language     = {eng},
  number       = {5},
  pages        = {441--451},
  title        = {Presence and dynamic redistribution of type I inositol 1,4,5-trisphosphate receptors in human oocytes and embryos during in-vitro maturation, fertilization and early cleavage divisions},
  url          = {http://molehr.oxfordjournals.org/cgi/content/abstract/5/5/441},
  volume       = {5},
  year         = {1999},
}

Chicago
Goud, Pravin T, Anuradha P Goud, Patric Van Oostveldt, and MARC DHONT. 1999. “Presence and Dynamic Redistribution of Type I Inositol 1,4,5-trisphosphate Receptors in Human Oocytes and Embryos During In-vitro Maturation, Fertilization and Early Cleavage Divisions.” Molecular Human Reproduction 5 (5): 441–451.
APA
Goud, P. T., Goud, A. P., Van Oostveldt, P., & DHONT, M. (1999). Presence and dynamic redistribution of type I inositol 1,4,5-trisphosphate receptors in human oocytes and embryos during in-vitro maturation, fertilization and early cleavage divisions. MOLECULAR HUMAN REPRODUCTION, 5(5), 441–451.
Vancouver
1.
Goud PT, Goud AP, Van Oostveldt P, DHONT M. Presence and dynamic redistribution of type I inositol 1,4,5-trisphosphate receptors in human oocytes and embryos during in-vitro maturation, fertilization and early cleavage divisions. MOLECULAR HUMAN REPRODUCTION. 1999;5(5):441–51.
MLA
Goud, Pravin T, Anuradha P Goud, Patric Van Oostveldt, et al. “Presence and Dynamic Redistribution of Type I Inositol 1,4,5-trisphosphate Receptors in Human Oocytes and Embryos During In-vitro Maturation, Fertilization and Early Cleavage Divisions.” MOLECULAR HUMAN REPRODUCTION 5.5 (1999): 441–451. Print.