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The Fps/Fes protein-tyrosine kinase promotes angiogenesis in transgenic mice

Peter Greer, Jody Haigh, Geraldine Mbamalu, Wilson Khoo, Alan Bernstein and Tony Pawson (1994) MOLECULAR AND CELLULAR BIOLOGY. 14(10). p.6755-6763
abstract
The fps/fes proto-oncogene encodes a cytoplasmic protein-tyrosine kinase known to be highly expressed in hematopoietic cells. To investigate fps/fes biological function, an activating mutation was introduced into the human fps/fes gene which directs amino-terminal myristylation of the Fps/Fes protein. This mutant, myristylated protein induced transformation of Rat-2 fibroblasts. The mutant fps/fes allele was incorporated into the mouse germ line and was found to be appropriately expressed in transgenic mice, in a tissue-specific pattern indistinguishable from that of the endogenous mouse gene. These mice displayed widespread hypervascularity, progressing to multifocal hemangiomas. High level of both the transgenic human and endogenous murine fps/fes transcripts were detected in vascular tumors by using RNase protection, and fps/fes transcripts were localized to endothelial cells of both the vascular tumors and normal blood vessels by in situ RNA hybridization. Primary human umbilical vein endothelial cultures were also shown to express fps/fes transcripts and the Fps/Fes tyrosine kinase. These results indicate that fps/fes expression is intrinsic to cells of the vascular endothelial lineage and suggest a direct role of the Fps/Fes protein-tyrosine kinase in the regulation of angiogenesis.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
V-FPS, SRC FAMILY, TRANSFORMING PROTEIN, AMINO-ACIDS, GROWTH-FACTOR, FES CDNA CLONES, MIDDLE-T-ONCOGENE, HUMAN-ENDOTHELIAL CELLS, COLONY-STIMULATING FACTOR, FUJINAMI SARCOMA-VIRUS
journal title
MOLECULAR AND CELLULAR BIOLOGY
Mol. Cell. Biol.
volume
14
issue
10
pages
6755 - 6763
Web of Science type
Article
ISSN
0270-7306
language
English
UGent publication?
no
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1202229
handle
http://hdl.handle.net/1854/LU-1202229
alternative location
http://mcb.asm.org/cgi/content/short/14/10/6755
date created
2011-04-05 11:50:06
date last changed
2016-12-19 15:45:49
@article{1202229,
  abstract     = {The fps/fes proto-oncogene encodes a cytoplasmic protein-tyrosine kinase known to be highly expressed in hematopoietic cells. To investigate fps/fes biological function, an activating mutation was introduced into the human fps/fes gene which directs amino-terminal myristylation of the Fps/Fes protein. This mutant, myristylated protein induced transformation of Rat-2 fibroblasts. The mutant fps/fes allele was incorporated into the mouse germ line and was found to be appropriately expressed in transgenic mice, in a tissue-specific pattern indistinguishable from that of the endogenous mouse gene. These mice displayed widespread hypervascularity, progressing to multifocal hemangiomas. High level of both the transgenic human and endogenous murine fps/fes transcripts were detected in vascular tumors by using RNase protection, and fps/fes transcripts were localized to endothelial cells of both the vascular tumors and normal blood vessels by in situ RNA hybridization. Primary human umbilical vein endothelial cultures were also shown to express fps/fes transcripts and the Fps/Fes tyrosine kinase. These results indicate that fps/fes expression is intrinsic to cells of the vascular endothelial lineage and suggest a direct role of the Fps/Fes protein-tyrosine kinase in the regulation of angiogenesis.},
  author       = {Greer, Peter and Haigh, Jody and Mbamalu, Geraldine and Khoo, Wilson and Bernstein, Alan and Pawson, Tony},
  issn         = {0270-7306},
  journal      = {MOLECULAR AND CELLULAR BIOLOGY},
  keyword      = {V-FPS,SRC FAMILY,TRANSFORMING PROTEIN,AMINO-ACIDS,GROWTH-FACTOR,FES CDNA CLONES,MIDDLE-T-ONCOGENE,HUMAN-ENDOTHELIAL CELLS,COLONY-STIMULATING FACTOR,FUJINAMI SARCOMA-VIRUS},
  language     = {eng},
  number       = {10},
  pages        = {6755--6763},
  title        = {The Fps/Fes protein-tyrosine kinase promotes angiogenesis in transgenic mice},
  url          = {http://mcb.asm.org/cgi/content/short/14/10/6755},
  volume       = {14},
  year         = {1994},
}

Chicago
Greer, Peter, Jody Haigh, Geraldine Mbamalu, Wilson Khoo, Alan Bernstein, and Tony Pawson. 1994. “The Fps/Fes Protein-tyrosine Kinase Promotes Angiogenesis in Transgenic Mice.” Molecular and Cellular Biology 14 (10): 6755–6763.
APA
Greer, P., Haigh, J., Mbamalu, G., Khoo, W., Bernstein, A., & Pawson, T. (1994). The Fps/Fes protein-tyrosine kinase promotes angiogenesis in transgenic mice. MOLECULAR AND CELLULAR BIOLOGY, 14(10), 6755–6763.
Vancouver
1.
Greer P, Haigh J, Mbamalu G, Khoo W, Bernstein A, Pawson T. The Fps/Fes protein-tyrosine kinase promotes angiogenesis in transgenic mice. MOLECULAR AND CELLULAR BIOLOGY. 1994;14(10):6755–63.
MLA
Greer, Peter, Jody Haigh, Geraldine Mbamalu, et al. “The Fps/Fes Protein-tyrosine Kinase Promotes Angiogenesis in Transgenic Mice.” MOLECULAR AND CELLULAR BIOLOGY 14.10 (1994): 6755–6763. Print.