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Pollen morphology as fertility predictor in hybrid tea roses

Luca Pipino UGent, Marie-Christine Van Labeke UGent, Andrea Mansuino, Valentina Scariot, Annalisa Giovannini and Leen Leus (2011) EUPHYTICA. 178(2). p.203-214
abstract
Fertility of hybrid tea roses is often reduced due to their interspecific origin but also to intensive inbreeding. New genotypes used as pollen donors represent an economic risk for a breeding programme, as their influence on seed production is unknown. In this study 11 cut rose genotypes were selected from a company database as high fertile or low fertile male parents, according to the number of seeds per hybridisation. Pollen morphology and in vitro germination of the selected genotypes were characterised. Pollen was either small (mean diameter < 30 mu m), shrunken, and irregular (abnormal), or large (mean diameter > 30 mu m), elliptical and crossed by furrows (normal). High correlations were found between the number of seeds produced per hybridisation and the pollen diameter (r = 0.94) or the percentage of normal pollen (r = 0.96). In order to evaluate the predictive power of the models, we conducted regression analyses and performed a validation experiment on genotypes not present in the database and without background information on fertility. Pollen diameter and percentage of normal pollen were characterised and fitted in the regression models for seed set predictions. Validation with an independent dataset gave a good prediction for 83.3% of the data. This indicates that using either the mean pollen diameter or the percentage of normal pollen resulted in effective fertility prediction. This tool could enhance the genetic variability in crossings between hybrid tea roses, thus creating possibilities for less economically risky exploitation of new tetraploid genotypes as male parents.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
GERMINATION, CROSSES, SEED SET, FRUIT, INHERITANCE, QUALITY, Breeding, Hybridisation, Pollen diameter, Rosa, Seed production
journal title
EUPHYTICA
Euphytica
volume
178
issue
2
pages
203 - 214
Web of Science type
Article
Web of Science id
000287457500006
JCR category
HORTICULTURE
JCR impact factor
1.554 (2011)
JCR rank
7/31 (2011)
JCR quartile
1 (2011)
ISSN
0014-2336
DOI
10.1007/s10681-010-0298-5
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1198814
handle
http://hdl.handle.net/1854/LU-1198814
date created
2011-03-29 19:57:32
date last changed
2011-04-29 15:17:14
@article{1198814,
  abstract     = {Fertility of hybrid tea roses is often reduced due to their interspecific origin but also to intensive inbreeding. New genotypes used as pollen donors represent an economic risk for a breeding programme, as their influence on seed production is unknown. In this study 11 cut rose genotypes were selected from a company database as high fertile or low fertile male parents, according to the number of seeds per hybridisation. Pollen morphology and in vitro germination of the selected genotypes were characterised. Pollen was either small (mean diameter {\textlangle} 30 mu m), shrunken, and irregular (abnormal), or large (mean diameter {\textrangle} 30 mu m), elliptical and crossed by furrows (normal). High correlations were found between the number of seeds produced per hybridisation and the pollen diameter (r = 0.94) or the percentage of normal pollen (r = 0.96). In order to evaluate the predictive power of the models, we conducted regression analyses and performed a validation experiment on genotypes not present in the database and without background information on fertility. Pollen diameter and percentage of normal pollen were characterised and fitted in the regression models for seed set predictions. Validation with an independent dataset gave a good prediction for 83.3\% of the data. This indicates that using either the mean pollen diameter or the percentage of normal pollen resulted in effective fertility prediction. This tool could enhance the genetic variability in crossings between hybrid tea roses, thus creating possibilities for less economically risky exploitation of new tetraploid genotypes as male parents.},
  author       = {Pipino, Luca and Van Labeke, Marie-Christine and Mansuino, Andrea and Scariot, Valentina and Giovannini, Annalisa and Leus, Leen},
  issn         = {0014-2336},
  journal      = {EUPHYTICA},
  keyword      = {GERMINATION,CROSSES,SEED SET,FRUIT,INHERITANCE,QUALITY,Breeding,Hybridisation,Pollen diameter,Rosa,Seed production},
  language     = {eng},
  number       = {2},
  pages        = {203--214},
  title        = {Pollen morphology as fertility predictor in hybrid tea roses},
  url          = {http://dx.doi.org/10.1007/s10681-010-0298-5},
  volume       = {178},
  year         = {2011},
}

Chicago
Pipino, Luca, Marie-Christine Van Labeke, Andrea Mansuino, Valentina Scariot, Annalisa Giovannini, and Leen Leus. 2011. “Pollen Morphology as Fertility Predictor in Hybrid Tea Roses.” Euphytica 178 (2): 203–214.
APA
Pipino, L., Van Labeke, M.-C., Mansuino, A., Scariot, V., Giovannini, A., & Leus, L. (2011). Pollen morphology as fertility predictor in hybrid tea roses. EUPHYTICA, 178(2), 203–214.
Vancouver
1.
Pipino L, Van Labeke M-C, Mansuino A, Scariot V, Giovannini A, Leus L. Pollen morphology as fertility predictor in hybrid tea roses. EUPHYTICA. 2011;178(2):203–14.
MLA
Pipino, Luca, Marie-Christine Van Labeke, Andrea Mansuino, et al. “Pollen Morphology as Fertility Predictor in Hybrid Tea Roses.” EUPHYTICA 178.2 (2011): 203–214. Print.