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iTRAQ protein quantification: a quality-controlled workflow

(2011) PROTEOMICS. 11(6). p.1125-1134
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Bioinformatics: from nucleotids to networks (N2N)
Abstract
Reporter ion-based methods are among the major techniques to quantify peptides and proteins. Two main labels, tandem mass tag (TMT) and iTRAQ, are widely used by the proteomics community. They are, however, often applied as out-of-the-box methods, without thorough quality control. Thus, due to undiscovered limitations of the technique, irrelevant results might be trusted. To address this issue, we here propose a step-by-step quality control of the iTRAQ workflow. From sample preparation to final ratio calculation we provide metrics and techniques assessing the actual effectiveness of iTRAQ quantification as well as a novel method for more reliable protein ratio estimation.
Keywords
ORBITRAP, STRATEGY, ABUNDANCE, DATABASES, IDENTIFICATION, PEPTIDE, ISOBARIC TAGS, COMPARATIVE PROTEOMICS, ABSOLUTE QUANTIFICATION, MASS-SPECTROMETRY, Quantification, Mass spectrometry, ORBITRAP, iTRAQ

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Citation

Please use this url to cite or link to this publication:

Chicago
Burkhart, Julia M, Marc Vaudel, ReneP Zahedi, Lennart Martens, and Albert Sickmann. 2011. “iTRAQ Protein Quantification: a Quality-controlled Workflow.” Proteomics 11 (6): 1125–1134.
APA
Burkhart, J. M., Vaudel, M., Zahedi, R., Martens, L., & Sickmann, A. (2011). iTRAQ protein quantification: a quality-controlled workflow. PROTEOMICS, 11(6), 1125–1134.
Vancouver
1.
Burkhart JM, Vaudel M, Zahedi R, Martens L, Sickmann A. iTRAQ protein quantification: a quality-controlled workflow. PROTEOMICS. 2011;11(6):1125–34.
MLA
Burkhart, Julia M, Marc Vaudel, ReneP Zahedi, et al. “iTRAQ Protein Quantification: a Quality-controlled Workflow.” PROTEOMICS 11.6 (2011): 1125–1134. Print.
@article{1197848,
  abstract     = {Reporter ion-based methods are among the major techniques to quantify peptides and proteins. Two main labels, tandem mass tag (TMT) and iTRAQ, are widely used by the proteomics community. They are, however, often applied as out-of-the-box methods, without thorough quality control. Thus, due to undiscovered limitations of the technique, irrelevant results might be trusted. To address this issue, we here propose a step-by-step quality control of the iTRAQ workflow. From sample preparation to final ratio calculation we provide metrics and techniques assessing the actual effectiveness of iTRAQ quantification as well as a novel method for more reliable protein ratio estimation.},
  author       = {Burkhart, Julia M and Vaudel, Marc and Zahedi, ReneP and Martens, Lennart and Sickmann, Albert},
  issn         = {1615-9853},
  journal      = {PROTEOMICS},
  keyword      = {ORBITRAP,STRATEGY,ABUNDANCE,DATABASES,IDENTIFICATION,PEPTIDE,ISOBARIC TAGS,COMPARATIVE PROTEOMICS,ABSOLUTE QUANTIFICATION,MASS-SPECTROMETRY,Quantification,Mass spectrometry,ORBITRAP,iTRAQ},
  language     = {eng},
  number       = {6},
  pages        = {1125--1134},
  title        = {iTRAQ protein quantification: a quality-controlled workflow},
  url          = {http://dx.doi.org/10.1002/pmic.201000711},
  volume       = {11},
  year         = {2011},
}

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