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In vivo-derived horse blastocysts show transcriptional upregulation of developmentally important genes compared with in vitro-produced horse blastocysts

Katrien Smits (UGent) , Karen Goossens, Ann Van Soom (UGent) , Jan Govaere (UGent) , Maarten Hoogewijs (UGent) and Luc Peelman (UGent)
(2011) REPRODUCTION FERTILITY AND DEVELOPMENT. 23(2). p.364-375
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Abstract
In vitro-produced (IVP) equine blastocysts can give rise to successful pregnancies, but their morphology and developmental rate differ from those of in vivo-derived equine blastocysts. The aim of the present study was to evaluate this difference at the genetic level. Suppression subtractive hybridisation (SSH) was used to construct a cDNA library enriched for transcripts preferentially expressed in in vivo-derived equine blastocysts compared with IVP blastocysts. Of the 62 different genes identified in this way, six genes involved in embryonic development (BEX2, FABP3, HSP90AA1, MOBKL3, MCM7 and ODC) were selected to confirm this differential expression by reverse transcription-quantitative real-time polymerase chain reaction (RT-qPCR). Using RT-qPCR, five genes were confirmed to be significantly upregulated in in vivo-derived blastocysts (i.e. FABP3, HSP90AA1 (both P<0.05), ODC, MOBKL3 and BEX2 (P<0.005 for all three)), confirming the results of the SSH. There was no significant difference in MCM7 expression between IVP and in vivo-derived blastocysts. In conclusion, five genes that are transcriptionally upregulated in in vivo-derived equine blastocysts compared with IVP blastocysts have been identified. Because of their possible importance in embryonic development, the expression of these genes can be used as a marker to evaluate in vitro embryo production systems in the horse.
Keywords
DNA-REPLICATION, EQUINE EMBRYOS, OOCYTE MATURATION, CULTURE-CONDITIONS, ORNITHINE-DECARBOXYLASE, BOVINE BLASTOCYSTS, MOUSE PREIMPLANTATION EMBRYOS, gene expression, MESSENGER-RNA EXPRESSION, CELL-SURVIVAL, PROTEINS

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MLA
Smits, Katrien, et al. “In Vivo-Derived Horse Blastocysts Show Transcriptional Upregulation of Developmentally Important Genes Compared with in Vitro-Produced Horse Blastocysts.” REPRODUCTION FERTILITY AND DEVELOPMENT, vol. 23, no. 2, 2011, pp. 364–75, doi:10.1071/RD10124.
APA
Smits, K., Goossens, K., Van Soom, A., Govaere, J., Hoogewijs, M., & Peelman, L. (2011). In vivo-derived horse blastocysts show transcriptional upregulation of developmentally important genes compared with in vitro-produced horse blastocysts. REPRODUCTION FERTILITY AND DEVELOPMENT, 23(2), 364–375. https://doi.org/10.1071/RD10124
Chicago author-date
Smits, Katrien, Karen Goossens, Ann Van Soom, Jan Govaere, Maarten Hoogewijs, and Luc Peelman. 2011. “In Vivo-Derived Horse Blastocysts Show Transcriptional Upregulation of Developmentally Important Genes Compared with in Vitro-Produced Horse Blastocysts.” REPRODUCTION FERTILITY AND DEVELOPMENT 23 (2): 364–75. https://doi.org/10.1071/RD10124.
Chicago author-date (all authors)
Smits, Katrien, Karen Goossens, Ann Van Soom, Jan Govaere, Maarten Hoogewijs, and Luc Peelman. 2011. “In Vivo-Derived Horse Blastocysts Show Transcriptional Upregulation of Developmentally Important Genes Compared with in Vitro-Produced Horse Blastocysts.” REPRODUCTION FERTILITY AND DEVELOPMENT 23 (2): 364–375. doi:10.1071/RD10124.
Vancouver
1.
Smits K, Goossens K, Van Soom A, Govaere J, Hoogewijs M, Peelman L. In vivo-derived horse blastocysts show transcriptional upregulation of developmentally important genes compared with in vitro-produced horse blastocysts. REPRODUCTION FERTILITY AND DEVELOPMENT. 2011;23(2):364–75.
IEEE
[1]
K. Smits, K. Goossens, A. Van Soom, J. Govaere, M. Hoogewijs, and L. Peelman, “In vivo-derived horse blastocysts show transcriptional upregulation of developmentally important genes compared with in vitro-produced horse blastocysts,” REPRODUCTION FERTILITY AND DEVELOPMENT, vol. 23, no. 2, pp. 364–375, 2011.
@article{1197648,
  abstract     = {{In vitro-produced (IVP) equine blastocysts can give rise to successful pregnancies, but their morphology and developmental rate differ from those of in vivo-derived equine blastocysts. The aim of the present study was to evaluate this difference at the genetic level. Suppression subtractive hybridisation (SSH) was used to construct a cDNA library enriched for transcripts preferentially expressed in in vivo-derived equine blastocysts compared with IVP blastocysts. Of the 62 different genes identified in this way, six genes involved in embryonic development (BEX2, FABP3, HSP90AA1, MOBKL3, MCM7 and ODC) were selected to confirm this differential expression by reverse transcription-quantitative real-time polymerase chain reaction (RT-qPCR). Using RT-qPCR, five genes were confirmed to be significantly upregulated in in vivo-derived blastocysts (i.e. FABP3, HSP90AA1 (both P<0.05), ODC, MOBKL3 and BEX2 (P<0.005 for all three)), confirming the results of the SSH. There was no significant difference in MCM7 expression between IVP and in vivo-derived blastocysts. In conclusion, five genes that are transcriptionally upregulated in in vivo-derived equine blastocysts compared with IVP blastocysts have been identified. Because of their possible importance in embryonic development, the expression of these genes can be used as a marker to evaluate in vitro embryo production systems in the horse.}},
  author       = {{Smits, Katrien and Goossens, Karen and Van Soom, Ann and Govaere, Jan and Hoogewijs, Maarten and Peelman, Luc}},
  issn         = {{1031-3613}},
  journal      = {{REPRODUCTION FERTILITY AND DEVELOPMENT}},
  keywords     = {{DNA-REPLICATION,EQUINE EMBRYOS,OOCYTE MATURATION,CULTURE-CONDITIONS,ORNITHINE-DECARBOXYLASE,BOVINE BLASTOCYSTS,MOUSE PREIMPLANTATION EMBRYOS,gene expression,MESSENGER-RNA EXPRESSION,CELL-SURVIVAL,PROTEINS}},
  language     = {{eng}},
  number       = {{2}},
  pages        = {{364--375}},
  title        = {{In vivo-derived horse blastocysts show transcriptional upregulation of developmentally important genes compared with in vitro-produced horse blastocysts}},
  url          = {{http://doi.org/10.1071/RD10124}},
  volume       = {{23}},
  year         = {{2011}},
}
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