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Identification of potentially involved proteins in levofloxacin resistance mechanisms in Coxiella burnetii

Iosif Vranakis, Pieter-Jan De Bock, Anastasia Papadioti, Yannis Tselentis, Kris Gevaert UGent, Georgios Tsiotis and Anna Psaroulaki (2011) JOURNAL OF PROTEOME RESEARCH. 10(2). p.756-762
abstract
The etiological agent of Q fever, Coxiella burnetii, is an obligate intracellular bacterium that multiplies within a phagosome-like parasitophorous vacuole. Fluoroquinolones have been used as an alternative therapy for Q fever. Resistance to fluoroquinolones can arise via several mechanisms utilized by pathogens to avoid killing. Until today, genome-based studies have shown that the main mechanism of C. burnetii to resist inhibition by fluoroquinolones is based on mutations in quinolone-resistance-determining region (QRDR). In this study, in a broader search at the protein level for C. burnetii mechanisms that confer resistance to fluoroquinolones, the proteomes of in vitro developed fluoroquinolone resistant bacteria and susceptible bacteria were compared using the MS-driven combined fractional diagonal chromatography (COFRADIC) proteomics technique. Quantitative comparison of the 381 proteins identified in both strains indicated the different expression of 15 bacterial proteins. These proteins are involved in different cellular processes indicating that the antibiotic resistance mechanism of the bacterium is a multifaceted process.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
TRANSFERASE, MS-driven proteomics, BIOSYNTHESIS, ANTIBIOTICS, GENE, DETERMINING REGION, DNA GYRASE, ESCHERICHIA-COLI, QUINOLONE-RESISTANCE, WHOLE-CELL LYSATE, COFRADIC, KeyWords Plus: Q-FEVER ENDOCARDITIS, antibiotic resistance, Coxiella burnetti, levofloxacin, fluoroquinolones
journal title
JOURNAL OF PROTEOME RESEARCH
J. Proteome Res.
volume
10
issue
2
pages
756 - 762
Web of Science type
Article
Web of Science id
000286868500035
JCR category
BIOCHEMICAL RESEARCH METHODS
JCR impact factor
5.113 (2011)
JCR rank
10/72 (2011)
JCR quartile
1 (2011)
ISSN
1535-3893
DOI
10.1021/pr100906v
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1197570
handle
http://hdl.handle.net/1854/LU-1197570
date created
2011-03-28 10:07:40
date last changed
2016-12-19 15:46:32
@article{1197570,
  abstract     = {The etiological agent of Q fever, Coxiella burnetii, is an obligate intracellular bacterium that multiplies within a phagosome-like parasitophorous vacuole. Fluoroquinolones have been used as an alternative therapy for Q fever. Resistance to fluoroquinolones can arise via several mechanisms utilized by pathogens to avoid killing. Until today, genome-based studies have shown that the main mechanism of C. burnetii to resist inhibition by fluoroquinolones is based on mutations in quinolone-resistance-determining region (QRDR). In this study, in a broader search at the protein level for C. burnetii mechanisms that confer resistance to fluoroquinolones, the proteomes of in vitro developed fluoroquinolone resistant bacteria and susceptible bacteria were compared using the MS-driven combined fractional diagonal chromatography (COFRADIC) proteomics technique. Quantitative comparison of the 381 proteins identified in both strains indicated the different expression of 15 bacterial proteins. These proteins are involved in different cellular processes indicating that the antibiotic resistance mechanism of the bacterium is a multifaceted process.},
  author       = {Vranakis, Iosif and De Bock, Pieter-Jan and Papadioti, Anastasia and Tselentis, Yannis and Gevaert, Kris and Tsiotis, Georgios and Psaroulaki, Anna},
  issn         = {1535-3893},
  journal      = {JOURNAL OF PROTEOME RESEARCH},
  keyword      = {TRANSFERASE,MS-driven proteomics,BIOSYNTHESIS,ANTIBIOTICS,GENE,DETERMINING REGION,DNA GYRASE,ESCHERICHIA-COLI,QUINOLONE-RESISTANCE,WHOLE-CELL LYSATE,COFRADIC,KeyWords Plus: Q-FEVER ENDOCARDITIS,antibiotic resistance,Coxiella burnetti,levofloxacin,fluoroquinolones},
  language     = {eng},
  number       = {2},
  pages        = {756--762},
  title        = {Identification of potentially involved proteins in levofloxacin resistance mechanisms in Coxiella burnetii},
  url          = {http://dx.doi.org/10.1021/pr100906v},
  volume       = {10},
  year         = {2011},
}

Chicago
Vranakis, Iosif, Pieter-Jan De Bock, Anastasia Papadioti, Yannis Tselentis, Kris Gevaert, Georgios Tsiotis, and Anna Psaroulaki. 2011. “Identification of Potentially Involved Proteins in Levofloxacin Resistance Mechanisms in Coxiella Burnetii.” Journal of Proteome Research 10 (2): 756–762.
APA
Vranakis, I., De Bock, P.-J., Papadioti, A., Tselentis, Y., Gevaert, K., Tsiotis, G., & Psaroulaki, A. (2011). Identification of potentially involved proteins in levofloxacin resistance mechanisms in Coxiella burnetii. JOURNAL OF PROTEOME RESEARCH, 10(2), 756–762.
Vancouver
1.
Vranakis I, De Bock P-J, Papadioti A, Tselentis Y, Gevaert K, Tsiotis G, et al. Identification of potentially involved proteins in levofloxacin resistance mechanisms in Coxiella burnetii. JOURNAL OF PROTEOME RESEARCH. 2011;10(2):756–62.
MLA
Vranakis, Iosif, Pieter-Jan De Bock, Anastasia Papadioti, et al. “Identification of Potentially Involved Proteins in Levofloxacin Resistance Mechanisms in Coxiella Burnetii.” JOURNAL OF PROTEOME RESEARCH 10.2 (2011): 756–762. Print.