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Identification of potentially involved proteins in levofloxacin resistance mechanisms in Coxiella burnetii

(2011) JOURNAL OF PROTEOME RESEARCH. 10(2). p.756-762
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Abstract
The etiological agent of Q fever, Coxiella burnetii, is an obligate intracellular bacterium that multiplies within a phagosome-like parasitophorous vacuole. Fluoroquinolones have been used as an alternative therapy for Q fever. Resistance to fluoroquinolones can arise via several mechanisms utilized by pathogens to avoid killing. Until today, genome-based studies have shown that the main mechanism of C. burnetii to resist inhibition by fluoroquinolones is based on mutations in quinolone-resistance-determining region (QRDR). In this study, in a broader search at the protein level for C. burnetii mechanisms that confer resistance to fluoroquinolones, the proteomes of in vitro developed fluoroquinolone resistant bacteria and susceptible bacteria were compared using the MS-driven combined fractional diagonal chromatography (COFRADIC) proteomics technique. Quantitative comparison of the 381 proteins identified in both strains indicated the different expression of 15 bacterial proteins. These proteins are involved in different cellular processes indicating that the antibiotic resistance mechanism of the bacterium is a multifaceted process.
Keywords
TRANSFERASE, MS-driven proteomics, BIOSYNTHESIS, ANTIBIOTICS, GENE, DETERMINING REGION, DNA GYRASE, ESCHERICHIA-COLI, QUINOLONE-RESISTANCE, WHOLE-CELL LYSATE, COFRADIC, KeyWords Plus: Q-FEVER ENDOCARDITIS, antibiotic resistance, Coxiella burnetti, levofloxacin, fluoroquinolones

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Chicago
Vranakis, Iosif, Pieter-Jan De Bock, Anastasia Papadioti, Yannis Tselentis, Kris Gevaert, Georgios Tsiotis, and Anna Psaroulaki. 2011. “Identification of Potentially Involved Proteins in Levofloxacin Resistance Mechanisms in Coxiella Burnetii.” Journal of Proteome Research 10 (2): 756–762.
APA
Vranakis, I., De Bock, P.-J., Papadioti, A., Tselentis, Y., Gevaert, K., Tsiotis, G., & Psaroulaki, A. (2011). Identification of potentially involved proteins in levofloxacin resistance mechanisms in Coxiella burnetii. JOURNAL OF PROTEOME RESEARCH, 10(2), 756–762.
Vancouver
1.
Vranakis I, De Bock P-J, Papadioti A, Tselentis Y, Gevaert K, Tsiotis G, et al. Identification of potentially involved proteins in levofloxacin resistance mechanisms in Coxiella burnetii. JOURNAL OF PROTEOME RESEARCH. 2011;10(2):756–62.
MLA
Vranakis, Iosif, Pieter-Jan De Bock, Anastasia Papadioti, et al. “Identification of Potentially Involved Proteins in Levofloxacin Resistance Mechanisms in Coxiella Burnetii.” JOURNAL OF PROTEOME RESEARCH 10.2 (2011): 756–762. Print.
@article{1197570,
  abstract     = {The etiological agent of Q fever, Coxiella burnetii, is an obligate intracellular bacterium that multiplies within a phagosome-like parasitophorous vacuole. Fluoroquinolones have been used as an alternative therapy for Q fever. Resistance to fluoroquinolones can arise via several mechanisms utilized by pathogens to avoid killing. Until today, genome-based studies have shown that the main mechanism of C. burnetii to resist inhibition by fluoroquinolones is based on mutations in quinolone-resistance-determining region (QRDR). In this study, in a broader search at the protein level for C. burnetii mechanisms that confer resistance to fluoroquinolones, the proteomes of in vitro developed fluoroquinolone resistant bacteria and susceptible bacteria were compared using the MS-driven combined fractional diagonal chromatography (COFRADIC) proteomics technique. Quantitative comparison of the 381 proteins identified in both strains indicated the different expression of 15 bacterial proteins. These proteins are involved in different cellular processes indicating that the antibiotic resistance mechanism of the bacterium is a multifaceted process.},
  author       = {Vranakis, Iosif and De Bock, Pieter-Jan and Papadioti, Anastasia and Tselentis, Yannis and Gevaert, Kris and Tsiotis, Georgios and Psaroulaki, Anna},
  issn         = {1535-3893},
  journal      = {JOURNAL OF PROTEOME RESEARCH},
  keyword      = {TRANSFERASE,MS-driven proteomics,BIOSYNTHESIS,ANTIBIOTICS,GENE,DETERMINING REGION,DNA GYRASE,ESCHERICHIA-COLI,QUINOLONE-RESISTANCE,WHOLE-CELL LYSATE,COFRADIC,KeyWords Plus: Q-FEVER ENDOCARDITIS,antibiotic resistance,Coxiella burnetti,levofloxacin,fluoroquinolones},
  language     = {eng},
  number       = {2},
  pages        = {756--762},
  title        = {Identification of potentially involved proteins in levofloxacin resistance mechanisms in Coxiella burnetii},
  url          = {http://dx.doi.org/10.1021/pr100906v},
  volume       = {10},
  year         = {2011},
}

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