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Flow cytometric differentiation of avian leukocytes and analysis of their intracellular cytokine expression

Sandra De Boever (UGent), Siska Croubels (UGent), Kristel Demeyere (UGent), Bart Lambrecht (UGent), Patrick De Backer (UGent) and Evelyne Meyer (UGent)
(2010) AVIAN PATHOLOGY. 39(1). p.41-46
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Abstract
A flow cytometric method for the identification of chicken blood leukocyte subpopulations and thrombocytes was developed. An anti-chicken CD45 phycoerythrin-labelled antibody was used to separate leukocytes from red blood cell nuclei. Leukocytes and thrombocytes were identified using a combination of their CD45-positivity and their typical side scatter properties. The identity of the CD45-positive cells was confirmed by sorting the subpopulations and subsequent light microscopic evaluation. In these differentiated cell populations, intracellular expression analysis of the proinflammatory cytokines interleukin-1 beta and interleukin-6 was subsequently optimized on whole blood after in vitro stimulation with lipopolysaccharide from Escherichia coli strain O127:B8.
Keywords
LIPOPOLYSACCHARIDE, MONOCLONAL-ANTIBODY, THROMBOCYTES, CELLS, INTERLEUKIN-6, ENDOTOXEMIA, LYMPHOCYTES, HETEROPHILS, WHOLE-BLOOD, TOLL-LIKE RECEPTOR-4

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Citation

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Chicago
De Boever, Sandra, Siska Croubels, Kristel Demeyere, Bart Lambrecht, Patrick De Backer, and Evelyne Meyer. 2010. “Flow Cytometric Differentiation of Avian Leukocytes and Analysis of Their Intracellular Cytokine Expression.” Avian Pathology 39 (1): 41–46.
APA
De Boever, S., Croubels, S., Demeyere, K., Lambrecht, B., De Backer, P., & Meyer, E. (2010). Flow cytometric differentiation of avian leukocytes and analysis of their intracellular cytokine expression. AVIAN PATHOLOGY, 39(1), 41–46.
Vancouver
1.
De Boever S, Croubels S, Demeyere K, Lambrecht B, De Backer P, Meyer E. Flow cytometric differentiation of avian leukocytes and analysis of their intracellular cytokine expression. AVIAN PATHOLOGY. 2010;39(1):41–6.
MLA
De Boever, Sandra, Siska Croubels, Kristel Demeyere, et al. “Flow Cytometric Differentiation of Avian Leukocytes and Analysis of Their Intracellular Cytokine Expression.” AVIAN PATHOLOGY 39.1 (2010): 41–46. Print.
@article{1195778,
  abstract     = {A flow cytometric method for the identification of chicken blood leukocyte subpopulations and thrombocytes was developed. An anti-chicken CD45 phycoerythrin-labelled antibody was used to separate leukocytes from red blood cell nuclei. Leukocytes and thrombocytes were identified using a combination of their CD45-positivity and their typical side scatter properties. The identity of the CD45-positive cells was confirmed by sorting the subpopulations and subsequent light microscopic evaluation. In these differentiated cell populations, intracellular expression analysis of the proinflammatory cytokines interleukin-1 beta and interleukin-6 was subsequently optimized on whole blood after in vitro stimulation with lipopolysaccharide from Escherichia coli strain O127:B8.},
  author       = {De Boever, Sandra and Croubels, Siska and Demeyere, Kristel and Lambrecht, Bart and De Backer, Patrick and Meyer, Evelyne},
  issn         = {0307-9457},
  journal      = {AVIAN PATHOLOGY},
  keyword      = {LIPOPOLYSACCHARIDE,MONOCLONAL-ANTIBODY,THROMBOCYTES,CELLS,INTERLEUKIN-6,ENDOTOXEMIA,LYMPHOCYTES,HETEROPHILS,WHOLE-BLOOD,TOLL-LIKE RECEPTOR-4},
  language     = {eng},
  number       = {1},
  pages        = {41--46},
  title        = {Flow cytometric differentiation of avian leukocytes and analysis of their intracellular cytokine expression},
  url          = {http://dx.doi.org/10.1080/03079450903473574},
  volume       = {39},
  year         = {2010},
}

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