Flow cytometric differentiation of avian leukocytes and analysis of their intracellular cytokine expression
- Author
- Sandra De Boever (UGent) , Siska Croubels (UGent) , Kristel Demeyere (UGent) , Bart Lambrecht (UGent) , Patrick De Backer (UGent) and Evelyne Meyer (UGent)
- Organization
- Abstract
- A flow cytometric method for the identification of chicken blood leukocyte subpopulations and thrombocytes was developed. An anti-chicken CD45 phycoerythrin-labelled antibody was used to separate leukocytes from red blood cell nuclei. Leukocytes and thrombocytes were identified using a combination of their CD45-positivity and their typical side scatter properties. The identity of the CD45-positive cells was confirmed by sorting the subpopulations and subsequent light microscopic evaluation. In these differentiated cell populations, intracellular expression analysis of the proinflammatory cytokines interleukin-1 beta and interleukin-6 was subsequently optimized on whole blood after in vitro stimulation with lipopolysaccharide from Escherichia coli strain O127:B8.
- Keywords
- LIPOPOLYSACCHARIDE, MONOCLONAL-ANTIBODY, THROMBOCYTES, CELLS, INTERLEUKIN-6, ENDOTOXEMIA, LYMPHOCYTES, HETEROPHILS, WHOLE-BLOOD, TOLL-LIKE RECEPTOR-4
Downloads
-
(...).pdf
- full text
- |
- UGent only
- |
- |
- 346.20 KB
Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-1195778
- MLA
- De Boever, Sandra, et al. “Flow Cytometric Differentiation of Avian Leukocytes and Analysis of Their Intracellular Cytokine Expression.” AVIAN PATHOLOGY, vol. 39, no. 1, 2010, pp. 41–46, doi:10.1080/03079450903473574.
- APA
- De Boever, S., Croubels, S., Demeyere, K., Lambrecht, B., De Backer, P., & Meyer, E. (2010). Flow cytometric differentiation of avian leukocytes and analysis of their intracellular cytokine expression. AVIAN PATHOLOGY, 39(1), 41–46. https://doi.org/10.1080/03079450903473574
- Chicago author-date
- De Boever, Sandra, Siska Croubels, Kristel Demeyere, Bart Lambrecht, Patrick De Backer, and Evelyne Meyer. 2010. “Flow Cytometric Differentiation of Avian Leukocytes and Analysis of Their Intracellular Cytokine Expression.” AVIAN PATHOLOGY 39 (1): 41–46. https://doi.org/10.1080/03079450903473574.
- Chicago author-date (all authors)
- De Boever, Sandra, Siska Croubels, Kristel Demeyere, Bart Lambrecht, Patrick De Backer, and Evelyne Meyer. 2010. “Flow Cytometric Differentiation of Avian Leukocytes and Analysis of Their Intracellular Cytokine Expression.” AVIAN PATHOLOGY 39 (1): 41–46. doi:10.1080/03079450903473574.
- Vancouver
- 1.De Boever S, Croubels S, Demeyere K, Lambrecht B, De Backer P, Meyer E. Flow cytometric differentiation of avian leukocytes and analysis of their intracellular cytokine expression. AVIAN PATHOLOGY. 2010;39(1):41–6.
- IEEE
- [1]S. De Boever, S. Croubels, K. Demeyere, B. Lambrecht, P. De Backer, and E. Meyer, “Flow cytometric differentiation of avian leukocytes and analysis of their intracellular cytokine expression,” AVIAN PATHOLOGY, vol. 39, no. 1, pp. 41–46, 2010.
@article{1195778, abstract = {{A flow cytometric method for the identification of chicken blood leukocyte subpopulations and thrombocytes was developed. An anti-chicken CD45 phycoerythrin-labelled antibody was used to separate leukocytes from red blood cell nuclei. Leukocytes and thrombocytes were identified using a combination of their CD45-positivity and their typical side scatter properties. The identity of the CD45-positive cells was confirmed by sorting the subpopulations and subsequent light microscopic evaluation. In these differentiated cell populations, intracellular expression analysis of the proinflammatory cytokines interleukin-1 beta and interleukin-6 was subsequently optimized on whole blood after in vitro stimulation with lipopolysaccharide from Escherichia coli strain O127:B8.}}, author = {{De Boever, Sandra and Croubels, Siska and Demeyere, Kristel and Lambrecht, Bart and De Backer, Patrick and Meyer, Evelyne}}, issn = {{0307-9457}}, journal = {{AVIAN PATHOLOGY}}, keywords = {{LIPOPOLYSACCHARIDE,MONOCLONAL-ANTIBODY,THROMBOCYTES,CELLS,INTERLEUKIN-6,ENDOTOXEMIA,LYMPHOCYTES,HETEROPHILS,WHOLE-BLOOD,TOLL-LIKE RECEPTOR-4}}, language = {{eng}}, number = {{1}}, pages = {{41--46}}, title = {{Flow cytometric differentiation of avian leukocytes and analysis of their intracellular cytokine expression}}, url = {{http://doi.org/10.1080/03079450903473574}}, volume = {{39}}, year = {{2010}}, }
- Altmetric
- View in Altmetric
- Web of Science
- Times cited: