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Flow cytometric differentiation of avian leukocytes and analysis of their intracellular cytokine expression

Sandra De Boever UGent, Siska Croubels UGent, Kristel Demeyere UGent, Bart Lambrecht UGent, Patrick De Backer UGent and Evelyne Meyer UGent (2010) AVIAN PATHOLOGY. 39(1). p.41-46
abstract
A flow cytometric method for the identification of chicken blood leukocyte subpopulations and thrombocytes was developed. An anti-chicken CD45 phycoerythrin-labelled antibody was used to separate leukocytes from red blood cell nuclei. Leukocytes and thrombocytes were identified using a combination of their CD45-positivity and their typical side scatter properties. The identity of the CD45-positive cells was confirmed by sorting the subpopulations and subsequent light microscopic evaluation. In these differentiated cell populations, intracellular expression analysis of the proinflammatory cytokines interleukin-1 beta and interleukin-6 was subsequently optimized on whole blood after in vitro stimulation with lipopolysaccharide from Escherichia coli strain O127:B8.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
LIPOPOLYSACCHARIDE, MONOCLONAL-ANTIBODY, THROMBOCYTES, CELLS, INTERLEUKIN-6, ENDOTOXEMIA, LYMPHOCYTES, HETEROPHILS, WHOLE-BLOOD, TOLL-LIKE RECEPTOR-4
journal title
AVIAN PATHOLOGY
Avian Pathol.
volume
39
issue
1
pages
41 - 46
Web of Science type
Article
Web of Science id
000273794000007
JCR category
VETERINARY SCIENCES
JCR impact factor
1.967 (2010)
JCR rank
16/145 (2010)
JCR quartile
1 (2010)
ISSN
0307-9457
DOI
10.1080/03079450903473574
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1195778
handle
http://hdl.handle.net/1854/LU-1195778
date created
2011-03-23 17:36:10
date last changed
2012-01-23 16:57:10
@article{1195778,
  abstract     = {A flow cytometric method for the identification of chicken blood leukocyte subpopulations and thrombocytes was developed. An anti-chicken CD45 phycoerythrin-labelled antibody was used to separate leukocytes from red blood cell nuclei. Leukocytes and thrombocytes were identified using a combination of their CD45-positivity and their typical side scatter properties. The identity of the CD45-positive cells was confirmed by sorting the subpopulations and subsequent light microscopic evaluation. In these differentiated cell populations, intracellular expression analysis of the proinflammatory cytokines interleukin-1 beta and interleukin-6 was subsequently optimized on whole blood after in vitro stimulation with lipopolysaccharide from Escherichia coli strain O127:B8.},
  author       = {De Boever, Sandra and Croubels, Siska and Demeyere, Kristel and Lambrecht, Bart and De Backer, Patrick and Meyer, Evelyne},
  issn         = {0307-9457},
  journal      = {AVIAN PATHOLOGY},
  keyword      = {LIPOPOLYSACCHARIDE,MONOCLONAL-ANTIBODY,THROMBOCYTES,CELLS,INTERLEUKIN-6,ENDOTOXEMIA,LYMPHOCYTES,HETEROPHILS,WHOLE-BLOOD,TOLL-LIKE RECEPTOR-4},
  language     = {eng},
  number       = {1},
  pages        = {41--46},
  title        = {Flow cytometric differentiation of avian leukocytes and analysis of their intracellular cytokine expression},
  url          = {http://dx.doi.org/10.1080/03079450903473574},
  volume       = {39},
  year         = {2010},
}

Chicago
De Boever, Sandra, Siska Croubels, Kristel Demeyere, Bart Lambrecht, Patrick De Backer, and Evelyne Meyer. 2010. “Flow Cytometric Differentiation of Avian Leukocytes and Analysis of Their Intracellular Cytokine Expression.” Avian Pathology 39 (1): 41–46.
APA
De Boever, S., Croubels, S., Demeyere, K., Lambrecht, B., De Backer, P., & Meyer, E. (2010). Flow cytometric differentiation of avian leukocytes and analysis of their intracellular cytokine expression. AVIAN PATHOLOGY, 39(1), 41–46.
Vancouver
1.
De Boever S, Croubels S, Demeyere K, Lambrecht B, De Backer P, Meyer E. Flow cytometric differentiation of avian leukocytes and analysis of their intracellular cytokine expression. AVIAN PATHOLOGY. 2010;39(1):41–6.
MLA
De Boever, Sandra, Siska Croubels, Kristel Demeyere, et al. “Flow Cytometric Differentiation of Avian Leukocytes and Analysis of Their Intracellular Cytokine Expression.” AVIAN PATHOLOGY 39.1 (2010): 41–46. Print.