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Detection of different quorum-sensing signal molecules in a virulent Edwardsiella tarda strain LTB-4

(2010) JOURNAL OF APPLIED MICROBIOLOGY. 108(1). p.139-147
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Abstract
Aims: The aim of this study was to elucidate the potential quorum-sensing (QS) signal molecules of an emerging pathogen (Edwardsiella tarda strain LTB-4) of cultured turbot (Scophthalmus maximus). Methods and Results: A sensitive and rapid double-layer plate method using biosensor strain Agrobacterium tumefaciens KYC55 was developed to detect the N-acylhomoserine lactone (AHL)-related compounds in bacteria. LTB-4 was found to have two QS systems, one was based on the AHLs and the other was based on the autoinducer-2 (AI-2). The AI-2 activity produced by LTB-4 was growth phase dependent and topped at OD600 of 1 center dot 0. The protocol to detect cholerae autoinducer 1 (CAI-1) activity in bacteria was modified, lowering the background luminescence of biosensor strain Vibrio harveyi JAF375. CAI-1 activity could not be detected in LTB-4. Conclusion: Edwardsiella tarda LTB-4 produced at least four kinds of AHLs during its whole growth phase. In comparison with the AHL-inducing QS, AI-2 may be the first predominant signal, functioning at early exponential phase. LTB-4 did not produce any CAI-1 activity. Significance and Impact of the Study: Different QS signal molecules of Edw. tarda LTB-4 were clarified by improved bioassays. In contrast to earlier studies detecting two types of AHLs, strain LTB-4 produced at least four kinds of AHLs, which seemed to be C-4-HSL, C-6-HSL, 3-oxo-C-6-HSL and an uncharacterized AHL molecule.
Keywords
cholerae autoinducer 1, N-acylhomoserine lactones, autoinducer 2, Edwardsiella tarda, quorum sensing, VIBRIO-HARVEYI, PSEUDOMONAS-AERUGINOSA, AEROMONAS-HYDROPHILA, GENE-EXPRESSION, LUXRI HOMOLOGS, LUMINESCENCE, AUTOINDUCER, SYSTEMS, IDENTIFICATION, INHIBITION

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Citation

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Chicago
Han, Yin, X Li, Zizhong Qi, XH Zhang, and Peter Bossier. 2010. “Detection of Different Quorum-sensing Signal Molecules in a Virulent Edwardsiella Tarda Strain LTB-4.” Journal of Applied Microbiology 108 (1): 139–147.
APA
Han, Y., Li, X., Qi, Z., Zhang, X., & Bossier, P. (2010). Detection of different quorum-sensing signal molecules in a virulent Edwardsiella tarda strain LTB-4. JOURNAL OF APPLIED MICROBIOLOGY, 108(1), 139–147.
Vancouver
1.
Han Y, Li X, Qi Z, Zhang X, Bossier P. Detection of different quorum-sensing signal molecules in a virulent Edwardsiella tarda strain LTB-4. JOURNAL OF APPLIED MICROBIOLOGY. 2010;108(1):139–47.
MLA
Han, Yin, X Li, Zizhong Qi, et al. “Detection of Different Quorum-sensing Signal Molecules in a Virulent Edwardsiella Tarda Strain LTB-4.” JOURNAL OF APPLIED MICROBIOLOGY 108.1 (2010): 139–147. Print.
@article{1191499,
  abstract     = {Aims:
The aim of this study was to elucidate the potential quorum-sensing (QS) signal molecules of an emerging pathogen (Edwardsiella tarda strain LTB-4) of cultured turbot (Scophthalmus maximus).
Methods and Results:
A sensitive and rapid double-layer plate method using biosensor strain Agrobacterium tumefaciens KYC55 was developed to detect the N-acylhomoserine lactone (AHL)-related compounds in bacteria. LTB-4 was found to have two QS systems, one was based on the AHLs and the other was based on the autoinducer-2 (AI-2). The AI-2 activity produced by LTB-4 was growth phase dependent and topped at OD600 of 1 center dot 0. The protocol to detect cholerae autoinducer 1 (CAI-1) activity in bacteria was modified, lowering the background luminescence of biosensor strain Vibrio harveyi JAF375. CAI-1 activity could not be detected in LTB-4.
Conclusion:
Edwardsiella tarda LTB-4 produced at least four kinds of AHLs during its whole growth phase. In comparison with the AHL-inducing QS, AI-2 may be the first predominant signal, functioning at early exponential phase. LTB-4 did not produce any CAI-1 activity.
Significance and Impact of the Study:
Different QS signal molecules of Edw. tarda LTB-4 were clarified by improved bioassays. In contrast to earlier studies detecting two types of AHLs, strain LTB-4 produced at least four kinds of AHLs, which seemed to be C-4-HSL, C-6-HSL, 3-oxo-C-6-HSL and an uncharacterized AHL molecule.},
  author       = {Han, Yin and Li, X and Qi, Zizhong and Zhang, XH and Bossier, Peter},
  issn         = {1364-5072},
  journal      = {JOURNAL OF APPLIED MICROBIOLOGY},
  keywords     = {cholerae autoinducer 1,N-acylhomoserine lactones,autoinducer 2,Edwardsiella tarda,quorum sensing,VIBRIO-HARVEYI,PSEUDOMONAS-AERUGINOSA,AEROMONAS-HYDROPHILA,GENE-EXPRESSION,LUXRI HOMOLOGS,LUMINESCENCE,AUTOINDUCER,SYSTEMS,IDENTIFICATION,INHIBITION},
  language     = {eng},
  number       = {1},
  pages        = {139--147},
  title        = {Detection of different quorum-sensing signal molecules in a virulent Edwardsiella tarda strain LTB-4},
  url          = {http://dx.doi.org/10.1111/j.1365-2672.2009.04405.x},
  volume       = {108},
  year         = {2010},
}

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