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Bacterial colonization of pellet softening reactors used during drinking water treatment

Frederik Hammes, Nico Boon UGent, Marius Vital, Petra Ross, Aleksandra Magic-Knezev and Marco Dignum (2011) APPLIED AND ENVIRONMENTAL MICROBIOLOGY. 77(3). p.1041-1048
abstract
Pellet softening reactors are used in centralized and decentralized drinking water treatment plants for the removal of calcium (hardness) through chemically induced precipitation of calcite. This is accomplished in fluidized pellet reactors, where a strong base is added to the influent to increase the pH and facilitate the process of precipitation on an added seeding material. Here we describe for the first time the opportunistic bacterial colonization of the calcite pellets in a full-scale pellet softening reactor and the functional contribution of these colonizing bacteria to the overall drinking water treatment process. ATP analysis, advanced microscopy, and community fingerprinting with denaturing gradient gel electrophoretic (DGGE) analysis were used to characterize the biomass on the pellets, while assimilable organic carbon (AOC), dissolved organic carbon, and flow cytometric analysis were used to characterize the impact of the biological processes on drinking water quality. The data revealed pellet colonization at concentrations in excess of 500 ng of ATP/g of pellet and reactor biomass concentrations as high as 220 mg of ATP/m(3) of reactor, comprising a wide variety of different microorganisms. These organisms removed as much as 60% of AOC from the water during treatment, thus contributing toward the biological stabilization of the drinking water. Notably, only a small fraction (about 60,000 cells/ml) of the bacteria in the reactors was released into the effluent under normal conditions, while the majority of the bacteria colonizing the pellets were captured in the calcite structures of the pellets and were removed as a reusable product.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
MICROBIAL ECOLOGY, REMOVAL, NANOFILTRATION, FLUIDIZED-BED, 16S RIBOSOMAL-RNA, GRADIENT GEL-ELECTROPHORESIS, GRANULAR ACTIVATED CARBON, ASSIMILABLE ORGANIC-CARBON, OPTIMIZATION, BIOMASS
journal title
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Appl. Environ. Microbiol.
volume
77
issue
3
pages
1041 - 1048
Web of Science type
Article
Web of Science id
000286597100040
JCR category
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
JCR impact factor
3.829 (2011)
JCR rank
29/157 (2011)
JCR quartile
1 (2011)
ISSN
0099-2240
DOI
10.1128/AEM.02068-10
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1189596
handle
http://hdl.handle.net/1854/LU-1189596
date created
2011-03-16 10:47:53
date last changed
2016-12-19 15:38:17
@article{1189596,
  abstract     = {Pellet softening reactors are used in centralized and decentralized drinking water treatment plants for the removal of calcium (hardness) through chemically induced precipitation of calcite. This is accomplished in fluidized pellet reactors, where a strong base is added to the influent to increase the pH and facilitate the process of precipitation on an added seeding material. Here we describe for the first time the opportunistic bacterial colonization of the calcite pellets in a full-scale pellet softening reactor and the functional contribution of these colonizing bacteria to the overall drinking water treatment process. ATP analysis, advanced microscopy, and community fingerprinting with denaturing gradient gel electrophoretic (DGGE) analysis were used to characterize the biomass on the pellets, while assimilable organic carbon (AOC), dissolved organic carbon, and flow cytometric analysis were used to characterize the impact of the biological processes on drinking water quality. The data revealed pellet colonization at concentrations in excess of 500 ng of ATP/g of pellet and reactor biomass concentrations as high as 220 mg of ATP/m(3) of reactor, comprising a wide variety of different microorganisms. These organisms removed as much as 60\% of AOC from the water during treatment, thus contributing toward the biological stabilization of the drinking water. Notably, only a small fraction (about 60,000 cells/ml) of the bacteria in the reactors was released into the effluent under normal conditions, while the majority of the bacteria colonizing the pellets were captured in the calcite structures of the pellets and were removed as a reusable product.},
  author       = {Hammes, Frederik and Boon, Nico and Vital, Marius and Ross, Petra and Magic-Knezev, Aleksandra and Dignum, Marco},
  issn         = {0099-2240},
  journal      = {APPLIED AND ENVIRONMENTAL MICROBIOLOGY},
  keyword      = {MICROBIAL ECOLOGY,REMOVAL,NANOFILTRATION,FLUIDIZED-BED,16S RIBOSOMAL-RNA,GRADIENT GEL-ELECTROPHORESIS,GRANULAR ACTIVATED CARBON,ASSIMILABLE ORGANIC-CARBON,OPTIMIZATION,BIOMASS},
  language     = {eng},
  number       = {3},
  pages        = {1041--1048},
  title        = {Bacterial colonization of pellet softening reactors used during drinking water treatment},
  url          = {http://dx.doi.org/10.1128/AEM.02068-10},
  volume       = {77},
  year         = {2011},
}

Chicago
Hammes, Frederik, Nico Boon, Marius Vital, Petra Ross, Aleksandra Magic-Knezev, and Marco Dignum. 2011. “Bacterial Colonization of Pellet Softening Reactors Used During Drinking Water Treatment.” Applied and Environmental Microbiology 77 (3): 1041–1048.
APA
Hammes, F., Boon, N., Vital, M., Ross, P., Magic-Knezev, A., & Dignum, M. (2011). Bacterial colonization of pellet softening reactors used during drinking water treatment. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 77(3), 1041–1048.
Vancouver
1.
Hammes F, Boon N, Vital M, Ross P, Magic-Knezev A, Dignum M. Bacterial colonization of pellet softening reactors used during drinking water treatment. APPLIED AND ENVIRONMENTAL MICROBIOLOGY. 2011;77(3):1041–8.
MLA
Hammes, Frederik, Nico Boon, Marius Vital, et al. “Bacterial Colonization of Pellet Softening Reactors Used During Drinking Water Treatment.” APPLIED AND ENVIRONMENTAL MICROBIOLOGY 77.3 (2011): 1041–1048. Print.