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Extranuclear protection of chromosomal DNA from oxidative stress

Sandy Vanderauwera UGent, Nobuhiro Suzuki, Gad Miller, Brigitte Van De Cotte UGent, Stijn Morsa UGent, Jean-Luc Ravanat, Alicia Hegie, Christian Triantaphylides, Vladimir Shulaev and Marc Van Montagu UGent, et al. (2011) PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 108(4). p.1711-1716
abstract
Eukaryotic organisms evolved under aerobic conditions subjecting nuclear DNA to damage provoked by reactive oxygen species (ROS). Although ROS are thought to be a major cause of DNA damage, little is known about the molecular mechanisms protecting nuclear DNA from oxidative stress. Here we show that protection of nuclear DNA in plants requires a coordinated function of ROS-scavenging pathways residing in the cytosol and peroxisomes, demonstrating that nuclear ROS scavengers such as peroxiredoxin and glutathione are insufficient to safeguard DNA integrity. Both catalase (CAT2) and cytosolic ascorbate peroxidase (APX1) play a key role in protecting the plant genome against photorespiratory-dependent H2O2-induced DNA damage. In apx1/cat2 double-mutant plants, a DNA damage response is activated, suppressing growth via a WEE1 kinase-dependent cell-cycle checkpoint. This response is correlated with enhanced tolerance to oxidative stress, DNA stress-causing agents, and inhibited programmed cell death.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
hydrogen peroxide, Arabidopsis, stress tolerance
journal title
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Proc. Natl. Acad. Sci. U. S. A.
volume
108
issue
4
pages
1711 - 1716
Web of Science type
Article
Web of Science id
000286594800091
JCR category
MULTIDISCIPLINARY SCIENCES
JCR impact factor
9.681 (2011)
JCR rank
3/54 (2011)
JCR quartile
1 (2011)
ISSN
0027-8424
DOI
10.1073/pnas.1018359108
project
Biotechnology for a sustainable economy (Bio-Economy)
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1188895
handle
http://hdl.handle.net/1854/LU-1188895
date created
2011-03-15 14:25:12
date last changed
2013-02-27 12:11:06
@article{1188895,
  abstract     = {Eukaryotic organisms evolved under aerobic conditions subjecting nuclear DNA to damage provoked by reactive oxygen species (ROS). Although ROS are thought to be a major cause of DNA damage, little is known about the molecular mechanisms protecting nuclear DNA from oxidative stress. Here we show that protection of nuclear DNA in plants requires a coordinated function of ROS-scavenging pathways residing in the cytosol and peroxisomes, demonstrating that nuclear ROS scavengers such as peroxiredoxin and glutathione are insufficient to safeguard DNA integrity. Both catalase (CAT2) and cytosolic ascorbate peroxidase (APX1) play a key role in protecting the plant genome against photorespiratory-dependent H2O2-induced DNA damage. In apx1/cat2 double-mutant plants, a DNA damage response is activated, suppressing growth via a WEE1 kinase-dependent cell-cycle checkpoint. This response is correlated with enhanced tolerance to oxidative stress, DNA stress-causing agents, and inhibited programmed cell death.},
  author       = {Vanderauwera, Sandy and Suzuki, Nobuhiro and Miller, Gad and Van De Cotte, Brigitte and Morsa, Stijn and Ravanat, Jean-Luc and Hegie, Alicia and Triantaphylides, Christian and Shulaev, Vladimir and Van Montagu, Marc and Van Breusegem, Frank and Mittler, Ron},
  issn         = {0027-8424},
  journal      = {PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA},
  keyword      = {hydrogen peroxide,Arabidopsis,stress tolerance},
  language     = {eng},
  number       = {4},
  pages        = {1711--1716},
  title        = {Extranuclear protection of chromosomal DNA from oxidative stress},
  url          = {http://dx.doi.org/10.1073/pnas.1018359108},
  volume       = {108},
  year         = {2011},
}

Chicago
Vanderauwera, Sandy, Nobuhiro Suzuki, Gad Miller, Brigitte Van De Cotte, Stijn Morsa, Jean-Luc Ravanat, Alicia Hegie, et al. 2011. “Extranuclear Protection of Chromosomal DNA from Oxidative Stress.” Proceedings of the National Academy of Sciences of the United States of America 108 (4): 1711–1716.
APA
Vanderauwera, S., Suzuki, N., Miller, G., Van De Cotte, B., Morsa, S., Ravanat, J.-L., Hegie, A., et al. (2011). Extranuclear protection of chromosomal DNA from oxidative stress. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 108(4), 1711–1716.
Vancouver
1.
Vanderauwera S, Suzuki N, Miller G, Van De Cotte B, Morsa S, Ravanat J-L, et al. Extranuclear protection of chromosomal DNA from oxidative stress. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 2011;108(4):1711–6.
MLA
Vanderauwera, Sandy, Nobuhiro Suzuki, Gad Miller, et al. “Extranuclear Protection of Chromosomal DNA from Oxidative Stress.” PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 108.4 (2011): 1711–1716. Print.