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Dissection of the phytohormonal regulation of trichome formation and biosynthesis of the antimalarial compound artemisinin in Artemisia annua plants

Lies Maes UGent, Filip Van Nieuwerburgh UGent, Yansheng Zhang, Darwin W Reed, Jacob Pollier UGent, Sofie Vande Casteele UGent, Dirk Inzé UGent, Patrick S Covello, Dieter Deforce UGent and Alain Goossens UGent (2011) NEW PHYTOLOGIST. 189(1). p.176-189
abstract
Biosynthesis of the sesquiterpene lactone and potent antimalarial drug artemisinin occurs in glandular trichomes of Artemisia annua plants and is subjected to a strict network of developmental and other regulatory cues. The effects of three hormones, jasmonate, gibberellin and cytokinin, were studied at the structural and molecular levels in two different A. annua chemotypes by microscopic analysis of gland development, and by targeted metabolite and transcript profiling. Furthermore, a genome-wide cDNA-amplified fragment length polymorphism (AFLP)-based transcriptome profiling was carried out of jasmonate-elicited leaves at different developmental stages. Although cytokinin and gibberellin positively affected at least one aspect of gland formation, these two hormones did not stimulate artemisinin biosynthesis. Only jasmonate simultaneously promoted gland formation and coordinated transcriptional activation of biosynthetic gene expression, which ultimately led to increased sesquiterpenoid accumulation with chemotype-dependent effects on the distinct pathway branches. Transcriptome profiling revealed a trichome-specific fatty acyl- coenzyme A reductase, trichome-specific fatty acyl-CoA reductase 1 (TFAR1), the expression of which correlates with trichome development and sesquiterpenoid biosynthesis. TFAR1 is potentially involved in cuticular wax formation during glandular trichome expansion in leaves and flowers of A. annua plants. Analysis of phytohormone-modulated transcriptional regulons provides clues to dissect the concerted regulation of metabolism and development of plant trichomes.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
MOLECULAR-CLONING, 11-DIENE SYNTHASE, AMORPHA-4, FATTY ACYL-COENZYME, IN-VIVO TRANSFORMATIONS, sesquiterpenoid, jasmonate, cDNA-AFLP transcript profiling, artemisinin, cytokinin, fatty acyl-CoA reductase, glandular trichome, gibberellin, JASMONIC ACID, KEY ENZYME, ARABIDOPSIS, DRUG ARTEMISININ, DIHYDROARTEMISINIC ACID, CELL-DIFFERENTIATION
journal title
NEW PHYTOLOGIST
New Phytol.
volume
189
issue
1
pages
176 - 189
Web of Science type
Article
Web of Science id
000284900100016
JCR category
PLANT SCIENCES
JCR impact factor
6.645 (2011)
JCR rank
6/189 (2011)
JCR quartile
1 (2011)
ISSN
0028-646X
DOI
10.1111/j.1469-8137.2010.03466.x
project
Biotechnology for a sustainable economy (Bio-Economy)
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1188855
handle
http://hdl.handle.net/1854/LU-1188855
date created
2011-03-15 14:24:44
date last changed
2013-02-27 12:08:09
@article{1188855,
  abstract     = {Biosynthesis of the sesquiterpene lactone and potent antimalarial drug artemisinin occurs in glandular trichomes of Artemisia annua plants and is subjected to a strict network of developmental and other regulatory cues.
The effects of three hormones, jasmonate, gibberellin and cytokinin, were studied at the structural and molecular levels in two different A. annua chemotypes by microscopic analysis of gland development, and by targeted metabolite and transcript profiling. Furthermore, a genome-wide cDNA-amplified fragment length polymorphism (AFLP)-based transcriptome profiling was carried out of jasmonate-elicited leaves at different developmental stages. Although cytokinin and gibberellin positively affected at least one aspect of gland formation, these two hormones did not stimulate artemisinin biosynthesis. Only jasmonate simultaneously promoted gland formation and coordinated transcriptional activation of biosynthetic gene expression, which ultimately led to increased sesquiterpenoid accumulation with chemotype-dependent effects on the distinct pathway branches. Transcriptome profiling revealed a trichome-specific fatty acyl- coenzyme A reductase, trichome-specific fatty acyl-CoA reductase 1 (TFAR1), the expression of which correlates with trichome development and sesquiterpenoid biosynthesis.
TFAR1 is potentially involved in cuticular wax formation during glandular trichome expansion in leaves and flowers of A. annua plants. Analysis of phytohormone-modulated transcriptional regulons provides clues to dissect the concerted regulation of metabolism and development of plant trichomes.},
  author       = {Maes, Lies and Van Nieuwerburgh, Filip and Zhang, Yansheng and Reed, Darwin W and Pollier, Jacob and Vande Casteele, Sofie and Inz{\'e}, Dirk and Covello, Patrick S and Deforce, Dieter and Goossens, Alain},
  issn         = {0028-646X},
  journal      = {NEW PHYTOLOGIST},
  keyword      = {MOLECULAR-CLONING,11-DIENE SYNTHASE,AMORPHA-4,FATTY ACYL-COENZYME,IN-VIVO TRANSFORMATIONS,sesquiterpenoid,jasmonate,cDNA-AFLP transcript profiling,artemisinin,cytokinin,fatty acyl-CoA reductase,glandular trichome,gibberellin,JASMONIC ACID,KEY ENZYME,ARABIDOPSIS,DRUG ARTEMISININ,DIHYDROARTEMISINIC ACID,CELL-DIFFERENTIATION},
  language     = {eng},
  number       = {1},
  pages        = {176--189},
  title        = {Dissection of the phytohormonal regulation of trichome formation and biosynthesis of the antimalarial compound artemisinin in Artemisia annua plants},
  url          = {http://dx.doi.org/10.1111/j.1469-8137.2010.03466.x},
  volume       = {189},
  year         = {2011},
}

Chicago
Maes, Lies, Filip Van Nieuwerburgh, Yansheng Zhang, Darwin W Reed, Jacob Pollier, Sofie Vande Casteele, Dirk Inzé, Patrick S Covello, Dieter Deforce, and Alain Goossens. 2011. “Dissection of the Phytohormonal Regulation of Trichome Formation and Biosynthesis of the Antimalarial Compound Artemisinin in Artemisia Annua Plants.” New Phytologist 189 (1): 176–189.
APA
Maes, Lies, Van Nieuwerburgh, F., Zhang, Y., Reed, D. W., Pollier, J., Vande Casteele, S., Inzé, D., et al. (2011). Dissection of the phytohormonal regulation of trichome formation and biosynthesis of the antimalarial compound artemisinin in Artemisia annua plants. NEW PHYTOLOGIST, 189(1), 176–189.
Vancouver
1.
Maes L, Van Nieuwerburgh F, Zhang Y, Reed DW, Pollier J, Vande Casteele S, et al. Dissection of the phytohormonal regulation of trichome formation and biosynthesis of the antimalarial compound artemisinin in Artemisia annua plants. NEW PHYTOLOGIST. 2011;189(1):176–89.
MLA
Maes, Lies, Filip Van Nieuwerburgh, Yansheng Zhang, et al. “Dissection of the Phytohormonal Regulation of Trichome Formation and Biosynthesis of the Antimalarial Compound Artemisinin in Artemisia Annua Plants.” NEW PHYTOLOGIST 189.1 (2011): 176–189. Print.