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Brassinosteroids control meristem size by promoting cell cycle progression in Arabidopsis roots

Mary-Paz González-García, Jpse Vilarrasa-Blasi, Miroslava Zhiponova UGent, Fanchon Divol, Santiago Mora-Garcií, Eugenia Russinova UGent and Ana I Caño-Delgado (2011) DEVELOPMENT. 138(5). p.849-859
abstract
Brassinosteroids (BRs) play crucial roles in plant growth and development. Previous studies have shown that BRs promote cell elongation in vegetative organs in several plant species, but their contribution to meristem homeostasis remains unexplored. Our analyses report that both loss- and gain-of-function BR-related mutants in Arabidopsis thaliana have reduced meristem size, indicating that balanced BR signalling is needed for the optimal root growth. In the BR-insensitive bri1-116 mutant, the expression pattern of the cell division markers CYCB1;1, ICK2/KRP2 and KNOLLE revealed that a decreased mitotic activity accounts for the reduced meristem size; accordingly, this defect could be overcome by the overexpression of CYCD3;1. The activity of the quiescent centre (QC) was low in the short roots of bri1-116, as reported by cell type-specific markers and differentiation phenotypes of distal stem cells. Conversely, plants treated with the most active BR, brassinolide, or mutants with enhanced BR signalling, such as bes1-D, show a premature cell cycle exit that results in early differentiation of meristematic cells, which also negatively influence meristem size and overall root growth. In the stem cell niche, BRs promote the QC renewal and differentiation of distal stem cells. Together, our results provide evidence that BRs play a regulatory role in the control of cell-cycle progression and differentiation in the Arabidopsis root meristem.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
Columella, Cell elongation, Stem cells, Quiescent centre, Cell division, REGULATED GENE-EXPRESSION, Meristem, Root, Brassinosteroids, SIGNAL-TRANSDUCTION, PLASMA-MEMBRANE, AUXIN TRANSPORT, GROWTH, THALIANA, RECEPTOR, KINASE, DIVISION, PROTEIN
journal title
DEVELOPMENT
Development
volume
138
issue
5
pages
849 - 859
Web of Science type
Article
Web of Science id
000287576100006
JCR category
DEVELOPMENTAL BIOLOGY
JCR impact factor
6.596 (2011)
JCR rank
5/40 (2011)
JCR quartile
1 (2011)
ISSN
0950-1991
DOI
10.1242/dev.057331
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1188797
handle
http://hdl.handle.net/1854/LU-1188797
date created
2011-03-15 14:24:03
date last changed
2016-12-19 15:45:16
@article{1188797,
  abstract     = {Brassinosteroids (BRs) play crucial roles in plant growth and development. Previous studies have shown that BRs promote cell elongation in vegetative organs in several plant species, but their contribution to meristem homeostasis remains unexplored. Our analyses report that both loss- and gain-of-function BR-related mutants in Arabidopsis thaliana have reduced meristem size, indicating that balanced BR signalling is needed for the optimal root growth. In the BR-insensitive bri1-116 mutant, the expression pattern of the cell division markers CYCB1;1, ICK2/KRP2 and KNOLLE revealed that a decreased mitotic activity accounts for the reduced meristem size; accordingly, this defect could be overcome by the overexpression of CYCD3;1. The activity of the quiescent centre (QC) was low in the short roots of bri1-116, as reported by cell type-specific markers and differentiation phenotypes of distal stem cells. Conversely, plants treated with the most active BR, brassinolide, or mutants with enhanced BR signalling, such as bes1-D, show a premature cell cycle exit that results in early differentiation of meristematic cells, which also negatively influence meristem size and overall root growth. In the stem cell niche, BRs promote the QC renewal and differentiation of distal stem cells. Together, our results provide evidence that BRs play a regulatory role in the control of cell-cycle progression and differentiation in the Arabidopsis root meristem.},
  author       = {Gonz{\'a}lez-Garc{\'i}a, Mary-Paz and Vilarrasa-Blasi, Jpse and Zhiponova, Miroslava and Divol, Fanchon and Mora-Garci{\'i}, Santiago and Russinova, Eugenia and Ca{\~n}o-Delgado, Ana I},
  issn         = {0950-1991},
  journal      = {DEVELOPMENT},
  keyword      = {Columella,Cell elongation,Stem cells,Quiescent centre,Cell division,REGULATED GENE-EXPRESSION,Meristem,Root,Brassinosteroids,SIGNAL-TRANSDUCTION,PLASMA-MEMBRANE,AUXIN TRANSPORT,GROWTH,THALIANA,RECEPTOR,KINASE,DIVISION,PROTEIN},
  language     = {eng},
  number       = {5},
  pages        = {849--859},
  title        = {Brassinosteroids control meristem size by promoting cell cycle progression in Arabidopsis roots},
  url          = {http://dx.doi.org/10.1242/dev.057331},
  volume       = {138},
  year         = {2011},
}

Chicago
González-García, Mary-Paz, Jpse Vilarrasa-Blasi, Miroslava Zhiponova, Fanchon Divol, Santiago Mora-Garcií, Eugenia Russinova, and Ana I Caño-Delgado. 2011. “Brassinosteroids Control Meristem Size by Promoting Cell Cycle Progression in Arabidopsis Roots.” Development 138 (5): 849–859.
APA
González-García, M.-P., Vilarrasa-Blasi, J., Zhiponova, M., Divol, F., Mora-Garcií, S., Russinova, E., & Caño-Delgado, A. I. (2011). Brassinosteroids control meristem size by promoting cell cycle progression in Arabidopsis roots. DEVELOPMENT, 138(5), 849–859.
Vancouver
1.
González-García M-P, Vilarrasa-Blasi J, Zhiponova M, Divol F, Mora-Garcií S, Russinova E, et al. Brassinosteroids control meristem size by promoting cell cycle progression in Arabidopsis roots. DEVELOPMENT. 2011;138(5):849–59.
MLA
González-García, Mary-Paz, Jpse Vilarrasa-Blasi, Miroslava Zhiponova, et al. “Brassinosteroids Control Meristem Size by Promoting Cell Cycle Progression in Arabidopsis Roots.” DEVELOPMENT 138.5 (2011): 849–859. Print.