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Alternatively activated macrophages and impaired phagocytosis of S-aureus in chronic rhinosinusitis

Olga Krysko UGent, Gabriële Holtappels UGent, Nan Zhang UGent, Malgorzata Kubica UGent, Kim Deswarte UGent, LARA DERYCKE UGent, Sofie Claeys UGent, Hamida Hammad UGent, Guy Brusselle UGent and Peter Vandenabeele UGent, et al. (2011) ALLERGY. 66(3). p.396-403
abstract
Chronic rhinosinusitis with nasal polyps (CRSwNP) is characterized by biased Th2 inflammation and CRS without nasal polyps (CRSsNP) by a Th1 immune response. Colonization by Staphylococcus aureus is increased in CRSwNP. We aimed to determine macrophage phenotypes in nasal mucosa of CRSwNP and CRSsNP and to examine phagocytosis of S. aureus in these pathologies. Methods: Macrophage phenotyping was performed by immunohistochemical staining on nasal mucosa sections from 28 patients; in addition flow cytometry analysis was performed. Tissue homogenate protein levels of IFN-gamma, IL-5, IL-6, IL-1 beta, TGF-beta, eosinophil cationic protein (ECP) and total IgE were analyzed and correlated with macrophage subtypes. Phagocytosis of S. aureus was analyzed by flow cytometry. Survival of S. aureus in Thp1 cells in the presence of polarizing cytokines was studied in vitro. Results: By immunohistochemical analysis more M2 macrophages were present in CRSwNP than in CRSsNP. This also was positively correlated with increased levels of IL-5, ECP and locally produced IgE and decreased levels of IL-6, IL-1 beta and IFN-gamma. FACS analysis of dissociated nasal tissue confirmed the presence of increased numbers of M2 macrophages (CD206+HLADR+CD14+CD11c+CD20-) in CRSwNP as compared to controls, while the number of M1 macrophages (CD206-HLADR+CD14+CD11cintCD16-CD20-) was not different. Phagocytosis of S. aureus by human tissue derived macrophages was reduced in CRSwNP as compared to macrophages from the control inferior turbinates. Conclusions:Decreased phagocytosis of S. aureus and an M2 activation phenotype in CRSwNP could potentially contribute to persistence of chronic inflammation in CRSwNP.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
innate immunity, macrophages, clinical immunology, ASTHMA, TISSUE, EXPRESSION, DISEASE, ENTEROTOXINS, POLARIZATION, DIFFERENTIATION, DENDRITIC CELLS, INFLAMMATORY MEDIATORS, NASAL POLYPS, nasal polyps, sinusitis
journal title
ALLERGY
Allergy
volume
66
issue
3
pages
396 - 403
Web of Science type
Article
Web of Science id
000286837100010
JCR category
ALLERGY
JCR impact factor
6.271 (2011)
JCR rank
2/23 (2011)
JCR quartile
1 (2011)
ISSN
0105-4538
DOI
10.1111/j.1398-9995.2010.02498.x
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1188623
handle
http://hdl.handle.net/1854/LU-1188623
date created
2011-03-15 11:34:36
date last changed
2015-06-17 09:30:40
@article{1188623,
  abstract     = {Chronic rhinosinusitis with nasal polyps (CRSwNP) is characterized by biased Th2 inflammation and CRS without nasal polyps (CRSsNP) by a Th1 immune response. Colonization by Staphylococcus aureus is increased in CRSwNP. We aimed to determine macrophage phenotypes in nasal mucosa of CRSwNP and CRSsNP and to examine phagocytosis of S. aureus in these pathologies. Methods: Macrophage phenotyping was performed by immunohistochemical staining on nasal mucosa sections from 28 patients; in addition flow cytometry analysis was performed. Tissue homogenate protein levels of IFN-gamma, IL-5, IL-6, IL-1 beta, TGF-beta, eosinophil cationic protein (ECP) and total IgE were analyzed and correlated with macrophage subtypes. Phagocytosis of S. aureus was analyzed by flow cytometry. Survival of S. aureus in Thp1 cells in the presence of polarizing cytokines was studied in vitro. Results: By immunohistochemical analysis more M2 macrophages were present in CRSwNP than in CRSsNP. This also was positively correlated with increased levels of IL-5, ECP and locally produced IgE and decreased levels of IL-6, IL-1 beta and IFN-gamma. FACS analysis of dissociated nasal tissue confirmed the presence of increased numbers of M2 macrophages (CD206+HLADR+CD14+CD11c+CD20-) in CRSwNP as compared to controls, while the number of M1 macrophages (CD206-HLADR+CD14+CD11cintCD16-CD20-) was not different. Phagocytosis of S. aureus by human tissue derived macrophages was reduced in CRSwNP as compared to macrophages from the control inferior turbinates. Conclusions:Decreased phagocytosis of S. aureus and an M2 activation phenotype in CRSwNP could potentially contribute to persistence of chronic inflammation in CRSwNP.},
  author       = {Krysko, Olga and Holtappels, Gabri{\"e}le and Zhang, Nan and Kubica, Malgorzata and Deswarte, Kim and DERYCKE, LARA and Claeys, Sofie and Hammad, Hamida and Brusselle, Guy and Vandenabeele, Peter and Krysko, Dmitri and Bachert, Claus},
  issn         = {0105-4538},
  journal      = {ALLERGY},
  keyword      = {innate immunity,macrophages,clinical immunology,ASTHMA,TISSUE,EXPRESSION,DISEASE,ENTEROTOXINS,POLARIZATION,DIFFERENTIATION,DENDRITIC CELLS,INFLAMMATORY MEDIATORS,NASAL POLYPS,nasal polyps,sinusitis},
  language     = {eng},
  number       = {3},
  pages        = {396--403},
  title        = {Alternatively activated macrophages and impaired phagocytosis of S-aureus in chronic rhinosinusitis},
  url          = {http://dx.doi.org/10.1111/j.1398-9995.2010.02498.x},
  volume       = {66},
  year         = {2011},
}

Chicago
Krysko, Olga, Gabriële Holtappels, Nan Zhang, Malgorzata Kubica, Kim Deswarte, LARA DERYCKE, Sofie Claeys, et al. 2011. “Alternatively Activated Macrophages and Impaired Phagocytosis of S-aureus in Chronic Rhinosinusitis.” Allergy 66 (3): 396–403.
APA
Krysko, O., Holtappels, G., Zhang, N., Kubica, M., Deswarte, K., DERYCKE, L., Claeys, S., et al. (2011). Alternatively activated macrophages and impaired phagocytosis of S-aureus in chronic rhinosinusitis. ALLERGY, 66(3), 396–403.
Vancouver
1.
Krysko O, Holtappels G, Zhang N, Kubica M, Deswarte K, DERYCKE L, et al. Alternatively activated macrophages and impaired phagocytosis of S-aureus in chronic rhinosinusitis. ALLERGY. 2011;66(3):396–403.
MLA
Krysko, Olga, Gabriële Holtappels, Nan Zhang, et al. “Alternatively Activated Macrophages and Impaired Phagocytosis of S-aureus in Chronic Rhinosinusitis.” ALLERGY 66.3 (2011): 396–403. Print.