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Antiserum against the conserved nine amino acid N-terminal peptide of influenza A virus matrix protein 2 is not immunoprotective

Marina De Filette UGent, Tine Ysenbaert UGent, Kenny Roose UGent, Michael Schotsaert UGent, Stefan Roels, Els Goossens, Bert Schepens UGent, Walther Fiers UGent and Xavier Saelens UGent (2011) JOURNAL OF GENERAL VIROLOGY. 92(2). p.301-306
abstract
The recent emergence and rapid spread of the pandemic H1N1 swine influenza virus reminded us once again of the need for a universal influenza vaccine that can elicit heterosubtypic protection. Here, we show the superior immunogenicity and immunoprotective capacity of the full-length matrix protein 2 ectodomain (M2e) peptide coupled to keyhole limpet haemocyanin (KLH) compared with the N-terminal 9 aa residues of M2e (SP1). Immunization with M2e KLH protected mice against a lethal challenge with influenza A virus and significantly reduced weight loss and lung virus titres. In addition, passive transfer of serum raised in rabbits against M2e KLH protected mice against a lethal influenza virus challenge, whereas serum from rabbits immunized with SP1 KLH did not. Nevertheless, immunofluorescence staining revealed that rabbit serum raised against SP1 KLH bound specifically to infected Madin Darby canine kidney cells. We conclude that the peptide SP1 contains an immunogenic epitope that is not sufficient for immunoprotection.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
MICE, M1, PROTECTION, ANTIBODY, VACCINE, EXTRACELLULAR DOMAIN, M2 PROTEIN
journal title
JOURNAL OF GENERAL VIROLOGY
J. Gen. Virol.
volume
92
issue
2
pages
301 - 306
Web of Science type
Article
Web of Science id
000287638800007
JCR category
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
JCR impact factor
3.363 (2011)
JCR rank
41/157 (2011)
JCR quartile
2 (2011)
ISSN
0022-1317
DOI
10.1099/vir.0.027086-0
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1187346
handle
http://hdl.handle.net/1854/LU-1187346
date created
2011-03-14 12:27:26
date last changed
2012-06-26 14:32:15
@article{1187346,
  abstract     = {The recent emergence and rapid spread of the pandemic H1N1 swine influenza virus reminded us once again of the need for a universal influenza vaccine that can elicit heterosubtypic protection. Here, we show the superior immunogenicity and immunoprotective capacity of the full-length matrix protein 2 ectodomain (M2e) peptide coupled to keyhole limpet haemocyanin (KLH) compared with the N-terminal 9 aa residues of M2e (SP1). Immunization with M2e KLH protected mice against a lethal challenge with influenza A virus and significantly reduced weight loss and lung virus titres. In addition, passive transfer of serum raised in rabbits against M2e KLH protected mice against a lethal influenza virus challenge, whereas serum from rabbits immunized with SP1 KLH did not. Nevertheless, immunofluorescence staining revealed that rabbit serum raised against SP1 KLH bound specifically to infected Madin Darby canine kidney cells. We conclude that the peptide SP1 contains an immunogenic epitope that is not sufficient for immunoprotection.},
  author       = {De Filette, Marina and Ysenbaert, Tine and Roose, Kenny and Schotsaert, Michael and Roels, Stefan and Goossens, Els and Schepens, Bert and Fiers, Walther and Saelens, Xavier},
  issn         = {0022-1317},
  journal      = {JOURNAL OF GENERAL VIROLOGY},
  keyword      = {MICE,M1,PROTECTION,ANTIBODY,VACCINE,EXTRACELLULAR DOMAIN,M2 PROTEIN},
  language     = {eng},
  number       = {2},
  pages        = {301--306},
  title        = {Antiserum against the conserved nine amino acid N-terminal peptide of influenza A virus matrix protein 2 is not immunoprotective},
  url          = {http://dx.doi.org/10.1099/vir.0.027086-0},
  volume       = {92},
  year         = {2011},
}

Chicago
De Filette, Marina, Tine Ysenbaert, Kenny Roose, Michael Schotsaert, Stefan Roels, Els Goossens, Bert Schepens, Walter Fiers, and Xavier Saelens. 2011. “Antiserum Against the Conserved Nine Amino Acid N-terminal Peptide of Influenza A Virus Matrix Protein 2 Is Not Immunoprotective.” Journal of General Virology 92 (2): 301–306.
APA
De Filette, M., Ysenbaert, T., Roose, K., Schotsaert, M., Roels, S., Goossens, E., Schepens, B., et al. (2011). Antiserum against the conserved nine amino acid N-terminal peptide of influenza A virus matrix protein 2 is not immunoprotective. JOURNAL OF GENERAL VIROLOGY, 92(2), 301–306.
Vancouver
1.
De Filette M, Ysenbaert T, Roose K, Schotsaert M, Roels S, Goossens E, et al. Antiserum against the conserved nine amino acid N-terminal peptide of influenza A virus matrix protein 2 is not immunoprotective. JOURNAL OF GENERAL VIROLOGY. 2011;92(2):301–6.
MLA
De Filette, Marina, Tine Ysenbaert, Kenny Roose, et al. “Antiserum Against the Conserved Nine Amino Acid N-terminal Peptide of Influenza A Virus Matrix Protein 2 Is Not Immunoprotective.” JOURNAL OF GENERAL VIROLOGY 92.2 (2011): 301–306. Print.