Advanced search
1 file | 392.66 KB

Antiserum against the conserved nine amino acid N-terminal peptide of influenza A virus matrix protein 2 is not immunoprotective

(2011) JOURNAL OF GENERAL VIROLOGY. 92(2). p.301-306
Author
Organization
Abstract
The recent emergence and rapid spread of the pandemic H1N1 swine influenza virus reminded us once again of the need for a universal influenza vaccine that can elicit heterosubtypic protection. Here, we show the superior immunogenicity and immunoprotective capacity of the full-length matrix protein 2 ectodomain (M2e) peptide coupled to keyhole limpet haemocyanin (KLH) compared with the N-terminal 9 aa residues of M2e (SP1). Immunization with M2e KLH protected mice against a lethal challenge with influenza A virus and significantly reduced weight loss and lung virus titres. In addition, passive transfer of serum raised in rabbits against M2e KLH protected mice against a lethal influenza virus challenge, whereas serum from rabbits immunized with SP1 KLH did not. Nevertheless, immunofluorescence staining revealed that rabbit serum raised against SP1 KLH bound specifically to infected Madin Darby canine kidney cells. We conclude that the peptide SP1 contains an immunogenic epitope that is not sufficient for immunoprotection.
Keywords
MICE, M1, PROTECTION, ANTIBODY, VACCINE, EXTRACELLULAR DOMAIN, M2 PROTEIN

Downloads

  • 1908 11DeFilette.pdf
    • full text
    • |
    • open access
    • |
    • PDF
    • |
    • 392.66 KB

Citation

Please use this url to cite or link to this publication:

Chicago
De Filette, Marina, Tine Ysenbaert, Kenny Roose, Michael Schotsaert, Stefan Roels, Els Goossens, Bert Schepens, Walter Fiers, and Xavier Saelens. 2011. “Antiserum Against the Conserved Nine Amino Acid N-terminal Peptide of Influenza A Virus Matrix Protein 2 Is Not Immunoprotective.” Journal of General Virology 92 (2): 301–306.
APA
De Filette, M., Ysenbaert, T., Roose, K., Schotsaert, M., Roels, S., Goossens, E., Schepens, B., et al. (2011). Antiserum against the conserved nine amino acid N-terminal peptide of influenza A virus matrix protein 2 is not immunoprotective. JOURNAL OF GENERAL VIROLOGY, 92(2), 301–306.
Vancouver
1.
De Filette M, Ysenbaert T, Roose K, Schotsaert M, Roels S, Goossens E, et al. Antiserum against the conserved nine amino acid N-terminal peptide of influenza A virus matrix protein 2 is not immunoprotective. JOURNAL OF GENERAL VIROLOGY. 2011;92(2):301–6.
MLA
De Filette, Marina et al. “Antiserum Against the Conserved Nine Amino Acid N-terminal Peptide of Influenza A Virus Matrix Protein 2 Is Not Immunoprotective.” JOURNAL OF GENERAL VIROLOGY 92.2 (2011): 301–306. Print.
@article{1187346,
  abstract     = {The recent emergence and rapid spread of the pandemic H1N1 swine influenza virus reminded us once again of the need for a universal influenza vaccine that can elicit heterosubtypic protection. Here, we show the superior immunogenicity and immunoprotective capacity of the full-length matrix protein 2 ectodomain (M2e) peptide coupled to keyhole limpet haemocyanin (KLH) compared with the N-terminal 9 aa residues of M2e (SP1). Immunization with M2e KLH protected mice against a lethal challenge with influenza A virus and significantly reduced weight loss and lung virus titres. In addition, passive transfer of serum raised in rabbits against M2e KLH protected mice against a lethal influenza virus challenge, whereas serum from rabbits immunized with SP1 KLH did not. Nevertheless, immunofluorescence staining revealed that rabbit serum raised against SP1 KLH bound specifically to infected Madin Darby canine kidney cells. We conclude that the peptide SP1 contains an immunogenic epitope that is not sufficient for immunoprotection.},
  author       = {De Filette, Marina and Ysenbaert, Tine and Roose, Kenny and Schotsaert, Michael and Roels, Stefan and Goossens, Els and Schepens, Bert and Fiers, Walter and Saelens, Xavier},
  issn         = {0022-1317},
  journal      = {JOURNAL OF GENERAL VIROLOGY},
  keywords     = {MICE,M1,PROTECTION,ANTIBODY,VACCINE,EXTRACELLULAR DOMAIN,M2 PROTEIN},
  language     = {eng},
  number       = {2},
  pages        = {301--306},
  title        = {Antiserum against the conserved nine amino acid N-terminal peptide of influenza A virus matrix protein 2 is not immunoprotective},
  url          = {http://dx.doi.org/10.1099/vir.0.027086-0},
  volume       = {92},
  year         = {2011},
}

Altmetric
View in Altmetric
Web of Science
Times cited: