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Respiratory burst activity in activated and unstimulated isolated bovine blood neutrophils during experimentally induced Escherichia coli mastitis.

K Van Oostveldt, Christian Burvenich UGent, FM Da Silva and AM Massart-Leen (1999) JOURNAL OF DAIRY RESEARCH. 66(3). p.375-383
abstract
The respiratory burst activity, measured as H2O2 production, of isolated bovine polymorphonuclear leucocytes (PMN) was evaluated during experimentally induced Escherichia coli mastitis by means of flow cytometry in cells activated by phorbol 12-myristate 13-acetate (PMA) and in unstimulated cells. As expected, a significantly reduced respiratory burst activity was observed in PMA-activated PMN 18 h after intramammary inoculation with Escherichia coli. At this time only 75% of the PMA-activated PMN showed a respiratory burst, but with a higher intensity than that measured before and later after infection with Esch. coli. In addition, an increase in the respiratory burst activity was observed in unstimulated blood PMN during a short period at 18 h after infection, when up to 30% of the unstimulated PMN had a respiratory burst activity. The increase in the respiratory burst intensity of PMA-activated PMN and the spontaneously augmented production of reduced oxygen species by the unstimulated PMN during infection with Esch. coli might indicate the production of a natural stimulator of burst activity in circulation, most probably originating from the inflamed udder.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
LEUKOCYTES, COWS, CYTOKINES
journal title
JOURNAL OF DAIRY RESEARCH
J. Dairy Res.
volume
66
issue
3
pages
375-383 pages
Web of Science type
Article
Web of Science id
000082131900004
ISSN
0022-0299
language
English
UGent publication?
yes
classification
A1
additional info
FT N/A
id
118718
handle
http://hdl.handle.net/1854/LU-118718
date created
2004-01-14 13:35:00
date last changed
2016-12-19 15:37:21
@article{118718,
  abstract     = {The respiratory burst activity, measured as H2O2 production, of isolated bovine polymorphonuclear leucocytes (PMN) was evaluated during experimentally induced Escherichia coli mastitis by means of flow cytometry in cells activated by phorbol 12-myristate 13-acetate (PMA) and in unstimulated cells. As expected, a significantly reduced respiratory burst activity was observed in PMA-activated PMN 18 h after intramammary inoculation with Escherichia coli. At this time only 75\% of the PMA-activated PMN showed a respiratory burst, but with a higher intensity than that measured before and later after infection with Esch. coli. In addition, an increase in the respiratory burst activity was observed in unstimulated blood PMN during a short period at 18 h after infection, when up to 30\% of the unstimulated PMN had a respiratory burst activity. The increase in the respiratory burst intensity of PMA-activated PMN and the spontaneously augmented production of reduced oxygen species by the unstimulated PMN during infection with Esch. coli might indicate the production of a natural stimulator of burst activity in circulation, most probably originating from the inflamed udder.},
  author       = {Van Oostveldt, K and Burvenich, Christian and Da Silva, FM and Massart-Leen, AM},
  issn         = {0022-0299},
  journal      = {JOURNAL OF DAIRY RESEARCH},
  keyword      = {LEUKOCYTES,COWS,CYTOKINES},
  language     = {eng},
  number       = {3},
  pages        = {375--383},
  title        = {Respiratory burst activity in activated and unstimulated isolated bovine blood neutrophils during experimentally induced Escherichia coli mastitis.},
  volume       = {66},
  year         = {1999},
}

Chicago
Van Oostveldt, K, Christian Burvenich, FM Da Silva, and AM Massart-Leen. 1999. “Respiratory Burst Activity in Activated and Unstimulated Isolated Bovine Blood Neutrophils During Experimentally Induced Escherichia Coli Mastitis.” Journal of Dairy Research 66 (3): 375–383.
APA
Van Oostveldt, K, Burvenich, C., Da Silva, F., & Massart-Leen, A. (1999). Respiratory burst activity in activated and unstimulated isolated bovine blood neutrophils during experimentally induced Escherichia coli mastitis. JOURNAL OF DAIRY RESEARCH, 66(3), 375–383.
Vancouver
1.
Van Oostveldt K, Burvenich C, Da Silva F, Massart-Leen A. Respiratory burst activity in activated and unstimulated isolated bovine blood neutrophils during experimentally induced Escherichia coli mastitis. JOURNAL OF DAIRY RESEARCH. 1999;66(3):375–83.
MLA
Van Oostveldt, K, Christian Burvenich, FM Da Silva, et al. “Respiratory Burst Activity in Activated and Unstimulated Isolated Bovine Blood Neutrophils During Experimentally Induced Escherichia Coli Mastitis.” JOURNAL OF DAIRY RESEARCH 66.3 (1999): 375–383. Print.