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Evaluation of the role of endotoxin and cortisol on modulation of CD18 adhesion receptors in cows with mastitis caused by Escherichia coli.

Eddy Roets, Christian Burvenich UGent, A Diez-Fraile and EN Noordhuizen-Stassen (1999) AMERICAN JOURNAL OF VETERINARY RESEARCH. 60(5). p.534-540
abstract
Objective-To determine the effect of mastitis caused by Escherichia coil on expression of CD18 cell surface receptors and to evaluate the involvement. and regulation of receptors by lipopolysaccharide (LPS) and cortisol. Animals-11 clinically normal lactating Holstein-Friesian cows. Procedure-Binding of CD18 monoclonal antibodies to neutrophils was studied, using flow cytometry, before and after intramammary inoculation of E coil organisms. Effect of LPS and cortisol on expression of adhesion receptors was investigated, using a whole-blood model. Results-Expression of CD18 adhesion receptors on bovine neutrophils increased 35% by 12 hours after intramammary inoculation of E coli By 24 hours after inoculation, the number of receptors had returned to control values. High cortisol concentrations (100 nmol/L) were seen 12 to 18 hours after inoculation. Addition of LPS to blood induced a 30% increase in the number of CD18 receptors, and maximal number of receptors was expressed at an LPS concentration of 0.1 ng/ml, A decrease in the number of CD18 receptors was induced by incubation with cortisol or dexamethasone before challenge-exposure with LPS. Conclusions-An increase in the number of CD18 receptors on neutrophils is mediated by local production of LPS, Subsequent endogenous release of cortisol may prevent additional increases in the number of receptors. Clinical Relevance-During acute mastitis caused by E coli, there is an increase in the number of CD18 receptors on circulating neutrophils. Cortisol induces a decrease in the number of CD18 receptors, probably modulating the acute inflammatory response in mammary glands of lactating cows.
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author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
STAPHYLOCOCCUS-AUREUS, POLYMORPHONUCLEAR LEUKOCYTES, ALVEOLAR MACROPHAGES, BOVINE NEUTROPHILS, DEXAMETHASONE, RELEASE, LACTATING DAIRY-COWS, NECROSIS-FACTOR-ALPHA, INVITRO, BLOOD
journal title
AMERICAN JOURNAL OF VETERINARY RESEARCH
Am. J. Vet. Res.
volume
60
issue
5
pages
534-540 pages
Web of Science type
Article
Web of Science id
000080115100017
ISSN
0002-9645
language
English
UGent publication?
yes
classification
A1
additional info
FT N/A
id
118703
handle
http://hdl.handle.net/1854/LU-118703
date created
2004-01-14 13:35:00
date last changed
2016-12-19 15:37:15
@article{118703,
  abstract     = {Objective-To determine the effect of mastitis caused by Escherichia coil on expression of CD18 cell surface receptors and to evaluate the involvement. and regulation of receptors by lipopolysaccharide (LPS) and cortisol.

Animals-11 clinically normal lactating Holstein-Friesian cows.

Procedure-Binding of CD18 monoclonal antibodies to neutrophils was studied, using flow cytometry, before and after intramammary inoculation of E coil organisms. Effect of LPS and cortisol on expression of adhesion receptors was investigated, using a whole-blood model.

Results-Expression of CD18 adhesion receptors on bovine neutrophils increased 35\% by 12 hours after intramammary inoculation of E coli By 24 hours after inoculation, the number of receptors had returned to control values. High cortisol concentrations (100 nmol/L) were seen 12 to 18 hours after inoculation. Addition of LPS to blood induced a 30\% increase in the number of CD18 receptors, and maximal number of receptors was expressed at an LPS concentration of 0.1 ng/ml, A decrease in the number of CD18 receptors was induced by incubation with cortisol or dexamethasone before challenge-exposure with LPS.

Conclusions-An increase in the number of CD18 receptors on neutrophils is mediated by local production of LPS, Subsequent endogenous release of cortisol may prevent additional increases in the number of receptors.

Clinical Relevance-During acute mastitis caused by E coli, there is an increase in the number of CD18 receptors on circulating neutrophils. Cortisol induces a decrease in the number of CD18 receptors, probably modulating the acute inflammatory response in mammary glands of lactating cows.},
  author       = {Roets, Eddy and Burvenich, Christian and Diez-Fraile, A and Noordhuizen-Stassen, EN},
  issn         = {0002-9645},
  journal      = {AMERICAN JOURNAL OF VETERINARY RESEARCH},
  keyword      = {STAPHYLOCOCCUS-AUREUS,POLYMORPHONUCLEAR LEUKOCYTES,ALVEOLAR MACROPHAGES,BOVINE NEUTROPHILS,DEXAMETHASONE,RELEASE,LACTATING DAIRY-COWS,NECROSIS-FACTOR-ALPHA,INVITRO,BLOOD},
  language     = {eng},
  number       = {5},
  pages        = {534--540},
  title        = {Evaluation of the role of endotoxin and cortisol on modulation of CD18 adhesion receptors in cows with mastitis caused by Escherichia coli.},
  volume       = {60},
  year         = {1999},
}

Chicago
Roets, Eddy, Christian Burvenich, A Diez-Fraile, and EN Noordhuizen-Stassen. 1999. “Evaluation of the Role of Endotoxin and Cortisol on Modulation of CD18 Adhesion Receptors in Cows with Mastitis Caused by Escherichia Coli.” American Journal of Veterinary Research 60 (5): 534–540.
APA
Roets, Eddy, Burvenich, C., Diez-Fraile, A., & Noordhuizen-Stassen, E. (1999). Evaluation of the role of endotoxin and cortisol on modulation of CD18 adhesion receptors in cows with mastitis caused by Escherichia coli. AMERICAN JOURNAL OF VETERINARY RESEARCH, 60(5), 534–540.
Vancouver
1.
Roets E, Burvenich C, Diez-Fraile A, Noordhuizen-Stassen E. Evaluation of the role of endotoxin and cortisol on modulation of CD18 adhesion receptors in cows with mastitis caused by Escherichia coli. AMERICAN JOURNAL OF VETERINARY RESEARCH. 1999;60(5):534–40.
MLA
Roets, Eddy, Christian Burvenich, A Diez-Fraile, et al. “Evaluation of the Role of Endotoxin and Cortisol on Modulation of CD18 Adhesion Receptors in Cows with Mastitis Caused by Escherichia Coli.” AMERICAN JOURNAL OF VETERINARY RESEARCH 60.5 (1999): 534–540. Print.