Ghent University Academic Bibliography

Advanced

Effect of bovine somatotropin on neutrophil functions and clinical symptoms during Streptococcus uberis mastitis.

Dagmar Hoeben, Christian Burvenich UGent, PJ Eppard, JC Byatt and DL Hard (1999) JOURNAL OF DAIRY SCIENCE. 82(7). p.1465-1481
abstract
The effect of recombinant bovine somatotropin (bST) on the chemiluminescence, diapedesis, and expression of adhesion receptors (CD11a, CD11b, CD18) of isolated polymorphonuclear leukocytes was studied. The plasma concentrations of insulin-like growth factor-I (IGF-I), bST, cortisol, and alpha-lactalbumin were also monitored. In addition, general and local clinical symptoms and the differentiation of circulating leukocytes were also studied during experimentally induced Streptococcus uberis mastitis in cows. Ten cows were infected with 500 cfu of S. uberis O140J in both left quarters. Five cows were subcutaneously treated with 500 mg of recombinant bST 7 d before and after infection, and 5 control cows received the excipient. General (fever, tachycardia, inappetance, and depression) and local symptoms (swelling, pain, firmness, and flecks in milk) were more acute, severe, and longer-lasting in control cows. Treatment with bST had no effect on chemiluminescence and diapedesis of circulating polymorphonuclear leukocytes and no effect on the expression of adhesion receptors. Recombinant bST induced significantly higher IGF-I and bST concentrations in plasma. The leukopenia observed after infection was less pronounced in the bST-treated cows, and the number of circulating band neutrophils and metamyelocytes was significantly lower in the treated group. The concentration of cortisol did not differ between both groups, but the blood concentration of alpha-lactalbumin significantly increased in both groups from 6 d after infection. These results showed that treatment with recombinant bST improves animal welfare by protecting the cows from severe local and general clinical symptoms during subsequent S. uberis mastitis, but that it has no effect on chemiluminescence, diapedesis, and the expression of adhesion receptors of circulating polymorphonuclear leukocytes.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
GROWTH-FACTOR-I, chemiluminescence, SUPEROXIDE ANION SECRETION, ESCHERICHIA-COLI MASTITIS, NECROSIS-FACTOR-ALPHA, LACTATING DAIRY-COWS, NEUROENDOCRINE HORMONES, EXOGENOUS SOMATOTROPIN, RESPIRATORY BURST, GAMMA-INTERFERON, OXIDATIVE BURST, diapedesis, Streptococcus uberis mastitis, recombinant bovine somatotropin
journal title
JOURNAL OF DAIRY SCIENCE
J. Dairy Sci.
volume
82
issue
7
pages
1465-1481 pages
Web of Science type
Article
Web of Science id
000081379200015
ISSN
0022-0302
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
118690
handle
http://hdl.handle.net/1854/LU-118690
alternative location
http://jds.fass.org/cgi/reprint/82/7/1465
date created
2004-01-14 13:35:00
date last changed
2016-12-19 15:37:15
@article{118690,
  abstract     = {The effect of recombinant bovine somatotropin (bST) on the chemiluminescence, diapedesis, and expression of adhesion receptors (CD11a, CD11b, CD18) of isolated polymorphonuclear leukocytes was studied. The plasma concentrations of insulin-like growth factor-I (IGF-I), bST, cortisol, and alpha-lactalbumin were also monitored. In addition, general and local clinical symptoms and the differentiation of circulating leukocytes were also studied during experimentally induced Streptococcus uberis mastitis in cows. Ten cows were infected with 500 cfu of S. uberis O140J in both left quarters. Five cows were subcutaneously treated with 500 mg of recombinant bST 7 d before and after infection, and 5 control cows received the excipient. General (fever, tachycardia, inappetance, and depression) and local symptoms (swelling, pain, firmness, and flecks in milk) were more acute, severe, and longer-lasting in control cows. Treatment with bST had no effect on chemiluminescence and diapedesis of circulating polymorphonuclear leukocytes and no effect on the expression of adhesion receptors. Recombinant bST induced significantly higher IGF-I and bST concentrations in plasma. The leukopenia observed after infection was less pronounced in the bST-treated cows, and the number of circulating band neutrophils and metamyelocytes was significantly lower in the treated group. The concentration of cortisol did not differ between both groups, but the blood concentration of alpha-lactalbumin significantly increased in both groups from 6 d after infection. These results showed that treatment with recombinant bST improves animal welfare by protecting the cows from severe local and general clinical symptoms during subsequent S. uberis mastitis, but that it has no effect on chemiluminescence, diapedesis, and the expression of adhesion receptors of circulating polymorphonuclear leukocytes.},
  author       = {Hoeben, Dagmar and Burvenich, Christian and Eppard, PJ and Byatt, JC and Hard, DL},
  issn         = {0022-0302},
  journal      = {JOURNAL OF DAIRY SCIENCE},
  keyword      = {GROWTH-FACTOR-I,chemiluminescence,SUPEROXIDE ANION SECRETION,ESCHERICHIA-COLI MASTITIS,NECROSIS-FACTOR-ALPHA,LACTATING DAIRY-COWS,NEUROENDOCRINE HORMONES,EXOGENOUS SOMATOTROPIN,RESPIRATORY BURST,GAMMA-INTERFERON,OXIDATIVE BURST,diapedesis,Streptococcus uberis mastitis,recombinant bovine somatotropin},
  language     = {eng},
  number       = {7},
  pages        = {1465--1481},
  title        = {Effect of bovine somatotropin on neutrophil functions and clinical symptoms during Streptococcus uberis mastitis.},
  url          = {http://jds.fass.org/cgi/reprint/82/7/1465},
  volume       = {82},
  year         = {1999},
}

Chicago
Hoeben, Dagmar, Christian Burvenich, PJ Eppard, JC Byatt, and DL Hard. 1999. “Effect of Bovine Somatotropin on Neutrophil Functions and Clinical Symptoms During Streptococcus Uberis Mastitis.” Journal of Dairy Science 82 (7): 1465–1481.
APA
Hoeben, Dagmar, Burvenich, C., Eppard, P., Byatt, J., & Hard, D. (1999). Effect of bovine somatotropin on neutrophil functions and clinical symptoms during Streptococcus uberis mastitis. JOURNAL OF DAIRY SCIENCE, 82(7), 1465–1481.
Vancouver
1.
Hoeben D, Burvenich C, Eppard P, Byatt J, Hard D. Effect of bovine somatotropin on neutrophil functions and clinical symptoms during Streptococcus uberis mastitis. JOURNAL OF DAIRY SCIENCE. 1999;82(7):1465–81.
MLA
Hoeben, Dagmar, Christian Burvenich, PJ Eppard, et al. “Effect of Bovine Somatotropin on Neutrophil Functions and Clinical Symptoms During Streptococcus Uberis Mastitis.” JOURNAL OF DAIRY SCIENCE 82.7 (1999): 1465–1481. Print.