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Genotyping and antimicrobial resistance patterns of Escherichia coli O157 originating from cattle farms

Katrijn Cobbaut UGent, Kurt Houf UGent, Filip Boyen UGent, Freddy Haesebrouck UGent and Lieven De Zutter UGent (2011) FOODBORNE PATHOGENS AND DISEASE. 8(6). p.719-724
abstract
During a Escherichia coli O157 prevalence study on cattle farms, 324 E. coli O157 isolates were collected from 68 out of 180 cattle farms. All isolates harbored the eaeA gene and the enterohemolysin (ehxA) gene. The majority of the strains only contained vtx2 (245 isolates), the combination of vtx1 and vtx2 was detected in 50 isolates, and in 29 isolates none of the vtx genes was present. Pulsed-field gel electrophoresis (PFGE) revealed that at a similarity level of 98% the isolates grouped into 83 different genotypes, 76 of which were only detected on one farm. Twenty-two out of the 68 positive farms harbored isolates belonging to more than one PFGE type, with a maximum of four different PFGE types. Minimal inhibitory concentrations of 10 antimicrobial agents were determined on a subset of 116 isolates, that is, one isolate per positive age category per farm. Acquired resistance to at least one antimicrobial agent was detected in 18 isolates and within a farm, only one resistance pattern was observed. All these 18 isolates were resistant toward streptomycin, and 16 of them also showed resistance toward sulfisoxazole. Six isolates were resistant to three or more antimicrobial agents.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
Genotyping, Antimicrobial Resistance, Cattle, Escherichia coli O157, HEMOLYTIC-UREMIC SYNDROME, DAIRY FARMS, ANTIBIOTIC-RESISTANCE, PREVALENCE, INFECTIONS, FOOD, SUSCEPTIBILITY, DIVERSITY, FEEDLOTS, HUMANS
journal title
FOODBORNE PATHOGENS AND DISEASE
Foodborne Pathog Dis
volume
8
issue
6
pages
719 - 724
Web of Science type
Article
Web of Science id
000291017100009
JCR category
FOOD SCIENCE & TECHNOLOGY
JCR impact factor
2.26 (2011)
JCR rank
27/128 (2011)
JCR quartile
1 (2011)
ISSN
1535-3141
DOI
10.1089/fpd.2010.0767
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1185949
handle
http://hdl.handle.net/1854/LU-1185949
date created
2011-03-10 17:02:22
date last changed
2016-12-19 15:46:38
@article{1185949,
  abstract     = {During a Escherichia coli O157 prevalence study on cattle farms, 324 E. coli O157 isolates were collected from 68 out of 180 cattle farms. All isolates harbored the eaeA gene and the enterohemolysin (ehxA) gene. The majority of the strains only contained vtx2 (245 isolates), the combination of vtx1 and vtx2 was detected in 50 isolates, and in 29 isolates none of the vtx genes was present. Pulsed-field gel electrophoresis (PFGE) revealed that at a similarity level of 98\% the isolates grouped into 83 different genotypes, 76 of which were only detected on one farm. Twenty-two out of the 68 positive farms harbored isolates belonging to more than one PFGE type, with a maximum of four different PFGE types. Minimal inhibitory concentrations of 10 antimicrobial agents were determined on a subset of 116 isolates, that is, one isolate per positive age category per farm. Acquired resistance to at least one antimicrobial agent was detected in 18 isolates and within a farm, only one resistance pattern was observed. All these 18 isolates were resistant toward streptomycin, and 16 of them also showed resistance toward sulfisoxazole. Six isolates were resistant to three or more antimicrobial agents.},
  author       = {Cobbaut, Katrijn and Houf, Kurt and Boyen, Filip and Haesebrouck, Freddy and De Zutter, Lieven},
  issn         = {1535-3141},
  journal      = {FOODBORNE PATHOGENS AND DISEASE},
  keyword      = {Genotyping,Antimicrobial Resistance,Cattle,Escherichia coli O157,HEMOLYTIC-UREMIC SYNDROME,DAIRY FARMS,ANTIBIOTIC-RESISTANCE,PREVALENCE,INFECTIONS,FOOD,SUSCEPTIBILITY,DIVERSITY,FEEDLOTS,HUMANS},
  language     = {eng},
  number       = {6},
  pages        = {719--724},
  title        = {Genotyping and antimicrobial resistance patterns of Escherichia coli O157 originating from cattle farms},
  url          = {http://dx.doi.org/10.1089/fpd.2010.0767},
  volume       = {8},
  year         = {2011},
}

Chicago
Cobbaut, Katrijn, Kurt Houf, Filip Boyen, Freddy Haesebrouck, and Lieven De Zutter. 2011. “Genotyping and Antimicrobial Resistance Patterns of Escherichia Coli O157 Originating from Cattle Farms.” Foodborne Pathogens and Disease 8 (6): 719–724.
APA
Cobbaut, K., Houf, K., Boyen, F., Haesebrouck, F., & De Zutter, L. (2011). Genotyping and antimicrobial resistance patterns of Escherichia coli O157 originating from cattle farms. FOODBORNE PATHOGENS AND DISEASE, 8(6), 719–724.
Vancouver
1.
Cobbaut K, Houf K, Boyen F, Haesebrouck F, De Zutter L. Genotyping and antimicrobial resistance patterns of Escherichia coli O157 originating from cattle farms. FOODBORNE PATHOGENS AND DISEASE. 2011;8(6):719–24.
MLA
Cobbaut, Katrijn, Kurt Houf, Filip Boyen, et al. “Genotyping and Antimicrobial Resistance Patterns of Escherichia Coli O157 Originating from Cattle Farms.” FOODBORNE PATHOGENS AND DISEASE 8.6 (2011): 719–724. Print.