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Genotyping and antimicrobial resistance patterns of Escherichia coli O157 originating from cattle farms

Katrijn Cobbaut (UGent) , Kurt Houf (UGent) , Filip Boyen (UGent) , Freddy Haesebrouck (UGent) and Lieven De Zutter (UGent)
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Abstract
During a Escherichia coli O157 prevalence study on cattle farms, 324 E. coli O157 isolates were collected from 68 out of 180 cattle farms. All isolates harbored the eaeA gene and the enterohemolysin (ehxA) gene. The majority of the strains only contained vtx2 (245 isolates), the combination of vtx1 and vtx2 was detected in 50 isolates, and in 29 isolates none of the vtx genes was present. Pulsed-field gel electrophoresis (PFGE) revealed that at a similarity level of 98% the isolates grouped into 83 different genotypes, 76 of which were only detected on one farm. Twenty-two out of the 68 positive farms harbored isolates belonging to more than one PFGE type, with a maximum of four different PFGE types. Minimal inhibitory concentrations of 10 antimicrobial agents were determined on a subset of 116 isolates, that is, one isolate per positive age category per farm. Acquired resistance to at least one antimicrobial agent was detected in 18 isolates and within a farm, only one resistance pattern was observed. All these 18 isolates were resistant toward streptomycin, and 16 of them also showed resistance toward sulfisoxazole. Six isolates were resistant to three or more antimicrobial agents.
Keywords
Genotyping, Antimicrobial Resistance, Cattle, Escherichia coli O157, HEMOLYTIC-UREMIC SYNDROME, DAIRY FARMS, ANTIBIOTIC-RESISTANCE, PREVALENCE, INFECTIONS, FOOD, SUSCEPTIBILITY, DIVERSITY, FEEDLOTS, HUMANS

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Chicago
Cobbaut, Katrijn, Kurt Houf, Filip Boyen, Freddy Haesebrouck, and Lieven De Zutter. 2011. “Genotyping and Antimicrobial Resistance Patterns of Escherichia Coli O157 Originating from Cattle Farms.” Foodborne Pathogens and Disease 8 (6): 719–724.
APA
Cobbaut, K., Houf, K., Boyen, F., Haesebrouck, F., & De Zutter, L. (2011). Genotyping and antimicrobial resistance patterns of Escherichia coli O157 originating from cattle farms. FOODBORNE PATHOGENS AND DISEASE, 8(6), 719–724.
Vancouver
1.
Cobbaut K, Houf K, Boyen F, Haesebrouck F, De Zutter L. Genotyping and antimicrobial resistance patterns of Escherichia coli O157 originating from cattle farms. FOODBORNE PATHOGENS AND DISEASE. 2011;8(6):719–24.
MLA
Cobbaut, Katrijn, Kurt Houf, Filip Boyen, et al. “Genotyping and Antimicrobial Resistance Patterns of Escherichia Coli O157 Originating from Cattle Farms.” FOODBORNE PATHOGENS AND DISEASE 8.6 (2011): 719–724. Print.
@article{1185949,
  abstract     = {During a Escherichia coli O157 prevalence study on cattle farms, 324 E. coli O157 isolates were collected from 68 out of 180 cattle farms. All isolates harbored the eaeA gene and the enterohemolysin (ehxA) gene. The majority of the strains only contained vtx2 (245 isolates), the combination of vtx1 and vtx2 was detected in 50 isolates, and in 29 isolates none of the vtx genes was present. Pulsed-field gel electrophoresis (PFGE) revealed that at a similarity level of 98\% the isolates grouped into 83 different genotypes, 76 of which were only detected on one farm. Twenty-two out of the 68 positive farms harbored isolates belonging to more than one PFGE type, with a maximum of four different PFGE types. Minimal inhibitory concentrations of 10 antimicrobial agents were determined on a subset of 116 isolates, that is, one isolate per positive age category per farm. Acquired resistance to at least one antimicrobial agent was detected in 18 isolates and within a farm, only one resistance pattern was observed. All these 18 isolates were resistant toward streptomycin, and 16 of them also showed resistance toward sulfisoxazole. Six isolates were resistant to three or more antimicrobial agents.},
  author       = {Cobbaut, Katrijn and Houf, Kurt and Boyen, Filip and Haesebrouck, Freddy and De Zutter, Lieven},
  issn         = {1535-3141},
  journal      = {FOODBORNE PATHOGENS AND DISEASE},
  keyword      = {Genotyping,Antimicrobial Resistance,Cattle,Escherichia coli O157,HEMOLYTIC-UREMIC SYNDROME,DAIRY FARMS,ANTIBIOTIC-RESISTANCE,PREVALENCE,INFECTIONS,FOOD,SUSCEPTIBILITY,DIVERSITY,FEEDLOTS,HUMANS},
  language     = {eng},
  number       = {6},
  pages        = {719--724},
  title        = {Genotyping and antimicrobial resistance patterns of Escherichia coli O157 originating from cattle farms},
  url          = {http://dx.doi.org/10.1089/fpd.2010.0767},
  volume       = {8},
  year         = {2011},
}

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