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Cross-protection between antigenically distinct H1N1 swine influenza viruses from Europe and North America

Annebel De Vleeschauwer UGent, Sjouke Van Poucke UGent, Alexander I Karasin, Christopher Olsen and Kristien Van Reeth UGent (2011) INFLUENZA AND OTHER RESPIRATORY VIRUSES. 5(2). p.115-122
abstract
Background An avian-like H1N1 swine influenza virus (SIV) is enzootic in swine populations of Western Europe. The virus is antigenically distinct from H1N1 SIVs in North America that have a classical swine virus-lineage H1 hemagglutinin, as does the pandemic (H1N1) 2009 virus. However, the significance of this antigenic difference for cross-protection among pigs remains unknown. Objectives We examined protection against infection with a North American triple reassortant H1N1 SIV [A/swine/Iowa/H04YS2/04 (sw/IA/04)] in pigs infected with a European avian-like SIV [A/swine/Belgium/1/98 (sw/B/98)] 4 weeks earlier. We also examined the genetic relationships and serologic cross-reactivity between both SIVs and with a pandemic (H1N1) 2009 virus [A/California/04/09 (Calif/09)]. Results After intranasal inoculation with sw/IA/04, all previously uninfected control pigs showed nasal virus excretion, high virus titers in the entire respiratory tract at 4 days post-challenge (DPCh) and macroscopic lung lesions. Most pigs previously infected with sw/B/98 tested negative for sw/IA/04 in nasal swabs and respiratory tissues, and none had lung lesions. At challenge, these pigs had low levels of cross-reactive virus neutralizing and neuraminidase inhibiting (NI) antibodies to sw/IA/04, but no hemagglutination-inhibiting antibodies. They showed similar antibody profiles when tested against Calif/09, but NI antibody titers were higher against Calif/09 than sw/IA/04, reflecting the higher genetic homology of the sw/B/98 neuraminidase with Calif/09. Conclusions Our data indicate that immunity induced by infection with European avian-like H1N1 SIV affords protection for pigs against North American H1N1 SIVs with a classical H1, and they suggest cross-protection against the pandemic (H1N1) 2009 virus.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
pandemic (H1N1) 2009, triple reassortant, H1N1 swine influenza virus, cross-protection, Avian-like, A VIRUS, PIGS, ORIGIN, H3N2, TRANSMISSION, INFECTION, EMERGENCE, SUBTYPES, ENGLAND, HUMANS
journal title
INFLUENZA AND OTHER RESPIRATORY VIRUSES
Influenza Other Respir. Viruses
volume
5
issue
2
pages
115 - 122
Web of Science type
Article
Web of Science id
000287196200008
JCR category
INFECTIOUS DISEASES
JCR impact factor
4.157 (2011)
JCR rank
13/70 (2011)
JCR quartile
1 (2011)
ISSN
1750-2640
DOI
10.1111/j.1750-2659.2010.00164.x
language
English
UGent publication?
yes
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
1185725
handle
http://hdl.handle.net/1854/LU-1185725
date created
2011-03-10 13:45:34
date last changed
2011-04-22 16:53:40
@article{1185725,
  abstract     = {Background An avian-like H1N1 swine influenza virus (SIV) is enzootic in swine populations of Western Europe. The virus is antigenically distinct from H1N1 SIVs in North America that have a classical swine virus-lineage H1 hemagglutinin, as does the pandemic (H1N1) 2009 virus. However, the significance of this antigenic difference for cross-protection among pigs remains unknown.
Objectives We examined protection against infection with a North American triple reassortant H1N1 SIV [A/swine/Iowa/H04YS2/04 (sw/IA/04)] in pigs infected with a European avian-like SIV [A/swine/Belgium/1/98 (sw/B/98)] 4 weeks earlier. We also examined the genetic relationships and serologic cross-reactivity between both SIVs and with a pandemic (H1N1) 2009 virus [A/California/04/09 (Calif/09)].
Results After intranasal inoculation with sw/IA/04, all previously uninfected control pigs showed nasal virus excretion, high virus titers in the entire respiratory tract at 4 days post-challenge (DPCh) and macroscopic lung lesions. Most pigs previously infected with sw/B/98 tested negative for sw/IA/04 in nasal swabs and respiratory tissues, and none had lung lesions. At challenge, these pigs had low levels of cross-reactive virus neutralizing and neuraminidase inhibiting (NI) antibodies to sw/IA/04, but no hemagglutination-inhibiting antibodies. They showed similar antibody profiles when tested against Calif/09, but NI antibody titers were higher against Calif/09 than sw/IA/04, reflecting the higher genetic homology of the sw/B/98 neuraminidase with Calif/09.
Conclusions Our data indicate that immunity induced by infection with European avian-like H1N1 SIV affords protection for pigs against North American H1N1 SIVs with a classical H1, and they suggest cross-protection against the pandemic (H1N1) 2009 virus.},
  author       = {De Vleeschauwer, Annebel and Van Poucke, Sjouke and Karasin, Alexander I and Olsen, Christopher  and Van Reeth, Kristien},
  issn         = {1750-2640},
  journal      = {INFLUENZA AND OTHER RESPIRATORY VIRUSES},
  keyword      = {pandemic (H1N1) 2009,triple reassortant,H1N1 swine influenza virus,cross-protection,Avian-like,A VIRUS,PIGS,ORIGIN,H3N2,TRANSMISSION,INFECTION,EMERGENCE,SUBTYPES,ENGLAND,HUMANS},
  language     = {eng},
  number       = {2},
  pages        = {115--122},
  title        = {Cross-protection between antigenically distinct H1N1 swine influenza viruses from Europe and North America},
  url          = {http://dx.doi.org/10.1111/j.1750-2659.2010.00164.x},
  volume       = {5},
  year         = {2011},
}

Chicago
De Vleeschauwer, Annebel, Sjouke Van Poucke, Alexander I Karasin, Christopher Olsen, and Kristien Van Reeth. 2011. “Cross-protection Between Antigenically Distinct H1N1 Swine Influenza Viruses from Europe and North America.” Influenza and Other Respiratory Viruses 5 (2): 115–122.
APA
De Vleeschauwer, A., Van Poucke, S., Karasin, A. I., Olsen, C., & Van Reeth, K. (2011). Cross-protection between antigenically distinct H1N1 swine influenza viruses from Europe and North America. INFLUENZA AND OTHER RESPIRATORY VIRUSES, 5(2), 115–122.
Vancouver
1.
De Vleeschauwer A, Van Poucke S, Karasin AI, Olsen C, Van Reeth K. Cross-protection between antigenically distinct H1N1 swine influenza viruses from Europe and North America. INFLUENZA AND OTHER RESPIRATORY VIRUSES. 2011;5(2):115–22.
MLA
De Vleeschauwer, Annebel, Sjouke Van Poucke, Alexander I Karasin, et al. “Cross-protection Between Antigenically Distinct H1N1 Swine Influenza Viruses from Europe and North America.” INFLUENZA AND OTHER RESPIRATORY VIRUSES 5.2 (2011): 115–122. Print.