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Carbon nanotubes-assisted polyacrylamide gel electrophoresis for enhanced separation of human serum proteins and application in liverish diagnosis

(2010) JOURNAL OF SEPARATION SCIENCE. 33(21). p.3393-3399
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Abstract
The application of pore-gradient polyacrylamide gel electrophoresis (PG-PAGE) incorporated with carbon nanotube modified by Triton X-100 and carboxylation so as to improve the separation of human serum proteins is reported. The novel PG-PAGE was made by adding water-soluble single-walled carbon nanotubes (CNTs) when preparing the polyacrylamide gel. Significant improvements in separation of complement C3 protein and haptoglobin (Hp) in human serum were achieved. It was estimated that the interactions between the hydrophilic groups on the proteins and the surface of the CNTs result in different adsorption kinetics of complement C3 and Hp subtype on the nanoparticles incorporated in the gel, thus enhancing the separation of the two proteins in serum. This new CNT matrix-assisted PG-PAGE method for enhanced separation of complement C3 and Hp in human serum was successfully applied to distinguish the samples from liverish patients and healthy people.
Keywords
Carbon nanotubes, Complement C3, Haptoglobin, Pore-gradient polyacrylamide gel electrophoresis, Serum protein, HEPATOCELLULAR-CARCINOMA, MASS-SPECTROMETRY, PROTEOMIC ANALYSIS, COMPLEMENT C3, DNA, AFFINITY, FUNCTIONALIZATION, IDENTIFICATION, BIOMARKERS, DELIVERY

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Chicago
Jiang, Fubin , Yanan Wang, Xinfang Hu, Na Shao, Na Na, Joris Delanghe, and Jin Ouyang. 2010. “Carbon Nanotubes-assisted Polyacrylamide Gel Electrophoresis for Enhanced Separation of Human Serum Proteins and Application in Liverish Diagnosis.” Journal of Separation Science 33 (21): 3393–3399.
APA
Jiang, F., Wang, Y., Hu, X., Shao, N., Na, N., Delanghe, J., & Ouyang, J. (2010). Carbon nanotubes-assisted polyacrylamide gel electrophoresis for enhanced separation of human serum proteins and application in liverish diagnosis. JOURNAL OF SEPARATION SCIENCE, 33(21), 3393–3399.
Vancouver
1.
Jiang F, Wang Y, Hu X, Shao N, Na N, Delanghe J, et al. Carbon nanotubes-assisted polyacrylamide gel electrophoresis for enhanced separation of human serum proteins and application in liverish diagnosis. JOURNAL OF SEPARATION SCIENCE. 2010;33(21):3393–9.
MLA
Jiang, Fubin , Yanan Wang, Xinfang Hu, et al. “Carbon Nanotubes-assisted Polyacrylamide Gel Electrophoresis for Enhanced Separation of Human Serum Proteins and Application in Liverish Diagnosis.” JOURNAL OF SEPARATION SCIENCE 33.21 (2010): 3393–3399. Print.
@article{1181506,
  abstract     = {The application of pore-gradient polyacrylamide gel electrophoresis (PG-PAGE) incorporated with carbon nanotube modified by Triton X-100 and carboxylation so as to improve the separation of human serum proteins is reported. The novel PG-PAGE was made by adding water-soluble single-walled carbon nanotubes (CNTs) when preparing the polyacrylamide gel. Significant improvements in separation of complement C3 protein and haptoglobin (Hp) in human serum were achieved. It was estimated that the interactions between the hydrophilic groups on the proteins and the surface of the CNTs result in different adsorption kinetics of complement C3 and Hp subtype on the nanoparticles incorporated in the gel, thus enhancing the separation of the two proteins in serum. This new CNT matrix-assisted PG-PAGE method for enhanced separation of complement C3 and Hp in human serum was successfully applied to distinguish the samples from liverish patients and healthy people.},
  author       = {Jiang, Fubin  and Wang, Yanan and Hu, Xinfang and Shao, Na and Na, Na and Delanghe, Joris and Ouyang, Jin},
  issn         = {1615-9306},
  journal      = {JOURNAL OF SEPARATION SCIENCE},
  keyword      = {Carbon nanotubes,Complement C3,Haptoglobin,Pore-gradient polyacrylamide gel electrophoresis,Serum protein,HEPATOCELLULAR-CARCINOMA,MASS-SPECTROMETRY,PROTEOMIC ANALYSIS,COMPLEMENT C3,DNA,AFFINITY,FUNCTIONALIZATION,IDENTIFICATION,BIOMARKERS,DELIVERY},
  language     = {eng},
  number       = {21},
  pages        = {3393--3399},
  title        = {Carbon nanotubes-assisted polyacrylamide gel electrophoresis for enhanced separation of human serum proteins and application in liverish diagnosis},
  url          = {http://dx.doi.org/10.1002/jssc.201000383},
  volume       = {33},
  year         = {2010},
}

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