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Carbon nanotubes-assisted polyacrylamide gel electrophoresis for enhanced separation of human serum proteins and application in liverish diagnosis

Fubin Jiang, Yanan Wang, Xinfang Hu, Na Shao, Na Na, Joris Delanghe UGent and Jin Ouyang (2010) JOURNAL OF SEPARATION SCIENCE. 33(21). p.3393-3399
abstract
The application of pore-gradient polyacrylamide gel electrophoresis (PG-PAGE) incorporated with carbon nanotube modified by Triton X-100 and carboxylation so as to improve the separation of human serum proteins is reported. The novel PG-PAGE was made by adding water-soluble single-walled carbon nanotubes (CNTs) when preparing the polyacrylamide gel. Significant improvements in separation of complement C3 protein and haptoglobin (Hp) in human serum were achieved. It was estimated that the interactions between the hydrophilic groups on the proteins and the surface of the CNTs result in different adsorption kinetics of complement C3 and Hp subtype on the nanoparticles incorporated in the gel, thus enhancing the separation of the two proteins in serum. This new CNT matrix-assisted PG-PAGE method for enhanced separation of complement C3 and Hp in human serum was successfully applied to distinguish the samples from liverish patients and healthy people.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
Carbon nanotubes, Complement C3, Haptoglobin, Pore-gradient polyacrylamide gel electrophoresis, Serum protein, HEPATOCELLULAR-CARCINOMA, MASS-SPECTROMETRY, PROTEOMIC ANALYSIS, COMPLEMENT C3, DNA, AFFINITY, FUNCTIONALIZATION, IDENTIFICATION, BIOMARKERS, DELIVERY
journal title
JOURNAL OF SEPARATION SCIENCE
J. Sep. Sci.
volume
33
issue
21
pages
3393 - 3399
Web of Science type
Article
Web of Science id
000284230700016
JCR category
CHEMISTRY, ANALYTICAL
JCR impact factor
2.631 (2010)
JCR rank
23/71 (2010)
JCR quartile
2 (2010)
ISSN
1615-9306
DOI
10.1002/jssc.201000383
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1181506
handle
http://hdl.handle.net/1854/LU-1181506
date created
2011-03-03 11:09:40
date last changed
2016-12-19 15:45:14
@article{1181506,
  abstract     = {The application of pore-gradient polyacrylamide gel electrophoresis (PG-PAGE) incorporated with carbon nanotube modified by Triton X-100 and carboxylation so as to improve the separation of human serum proteins is reported. The novel PG-PAGE was made by adding water-soluble single-walled carbon nanotubes (CNTs) when preparing the polyacrylamide gel. Significant improvements in separation of complement C3 protein and haptoglobin (Hp) in human serum were achieved. It was estimated that the interactions between the hydrophilic groups on the proteins and the surface of the CNTs result in different adsorption kinetics of complement C3 and Hp subtype on the nanoparticles incorporated in the gel, thus enhancing the separation of the two proteins in serum. This new CNT matrix-assisted PG-PAGE method for enhanced separation of complement C3 and Hp in human serum was successfully applied to distinguish the samples from liverish patients and healthy people.},
  author       = {Jiang, Fubin  and Wang, Yanan and Hu, Xinfang and Shao, Na and Na, Na and Delanghe, Joris and Ouyang, Jin},
  issn         = {1615-9306},
  journal      = {JOURNAL OF SEPARATION SCIENCE},
  keyword      = {Carbon nanotubes,Complement C3,Haptoglobin,Pore-gradient polyacrylamide gel electrophoresis,Serum protein,HEPATOCELLULAR-CARCINOMA,MASS-SPECTROMETRY,PROTEOMIC ANALYSIS,COMPLEMENT C3,DNA,AFFINITY,FUNCTIONALIZATION,IDENTIFICATION,BIOMARKERS,DELIVERY},
  language     = {eng},
  number       = {21},
  pages        = {3393--3399},
  title        = {Carbon nanotubes-assisted polyacrylamide gel electrophoresis for enhanced separation of human serum proteins and application in liverish diagnosis},
  url          = {http://dx.doi.org/10.1002/jssc.201000383},
  volume       = {33},
  year         = {2010},
}

Chicago
Jiang, Fubin , Yanan Wang, Xinfang Hu, Na Shao, Na Na, Joris Delanghe, and Jin Ouyang. 2010. “Carbon Nanotubes-assisted Polyacrylamide Gel Electrophoresis for Enhanced Separation of Human Serum Proteins and Application in Liverish Diagnosis.” Journal of Separation Science 33 (21): 3393–3399.
APA
Jiang, F., Wang, Y., Hu, X., Shao, N., Na, N., Delanghe, J., & Ouyang, J. (2010). Carbon nanotubes-assisted polyacrylamide gel electrophoresis for enhanced separation of human serum proteins and application in liverish diagnosis. JOURNAL OF SEPARATION SCIENCE, 33(21), 3393–3399.
Vancouver
1.
Jiang F, Wang Y, Hu X, Shao N, Na N, Delanghe J, et al. Carbon nanotubes-assisted polyacrylamide gel electrophoresis for enhanced separation of human serum proteins and application in liverish diagnosis. JOURNAL OF SEPARATION SCIENCE. 2010;33(21):3393–9.
MLA
Jiang, Fubin , Yanan Wang, Xinfang Hu, et al. “Carbon Nanotubes-assisted Polyacrylamide Gel Electrophoresis for Enhanced Separation of Human Serum Proteins and Application in Liverish Diagnosis.” JOURNAL OF SEPARATION SCIENCE 33.21 (2010): 3393–3399. Print.