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Evolution of vitamin D binding protein concentration in sera from cardiac surgery patients is determined by triglyceridemia

Marijn Speeckaert UGent, Charline Wehlou, Filip De Somer UGent, Reinhart Speeckaert, Guido Van Nooten UGent and Joris Delanghe UGent (2010) CLINICAL CHEMISTRY AND LABORATORY MEDICINE. 48(9). p.1345-1350
abstract
Background: Multiple human and animal studies have reviewed the (non-)actin scavenger functions of vitamin D binding protein (DBP). Recently, we demonstrated the partially lipid bound character of DBP. The purpose of the present study was to explore the link between actin, lipids and DBP in a cohort of patients undergoing cardiac surgery. Methods: The interplay between DBP, actin and lipids was investigated in a cohort study of 35 Caucasian patients who underwent cardiac surgery. Total and actin-free DBP concentrations were assessed by immunonephelometry and ELISA. Total cholesterol, high-density lipoprotein (HDL) cholesterol, triglycerides, aspartate aminotransferase, alanine aminotransferase, lactate dehydrogenase, creatine kinase (-MB) and C-reactive protein (CRP) were measured using routine methods. Low-density lipoprotein cholesterol was calculated according to the Friedewald formula. The DBP phenotypes and the macromolecular bound DBP fractions were determined by polyacrylamide gel electrophoresis (PAGE) and confirmed by Western blotting. Results: Using PAGE and Western blotting with an antihuman DBP polyclonal antibody, the actin-bound DBP complex was identified in serum. Following cardiac surgery, total serum DBP concentrations were characterized by a two phased course. This was paralleled by a similar pattern in serum total cholesterol, HDL-cholesterol and triglyceride concentrations. Good correlation was found between total and actin free serum DBP concentrations (r=0.69, p<0.0001). The serum actin free DBP/total DBP ratio remained stable throughout the study period. Although no significant correlation between cumulative CK-MB enzyme release and delta serum total DBP concentration was observed (p=NS), the latter value correlated significantly with delta serum triglyceride concentrations (r=0.37, p<0.05). Conclusions: The lipid bound character is an underestimated property of DBP in the extracellular actin-scavenger system. Clin Chem Lab Med 2010;48:1345-50.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
vitamin D binding protein, lipids, cardiac surgery, actin scavenging, CATALYTIC ACTIVITY CONCENTRATIONS, GROUP-SPECIFIC COMPONENT, GC-GLOBULIN, CARDIOPULMONARY BYPASS, CHOLESTEROL, 37-DEGREES-C, ENZYMES, ACTIN, AMINOTRANSFERASE, COMPLEXES
journal title
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
Clin. Chem. Lab. Med.
volume
48
issue
9
pages
1345 - 1350
Web of Science type
Article
Web of Science id
000283183300024
JCR category
MEDICAL LABORATORY TECHNOLOGY
JCR impact factor
2.069 (2010)
JCR rank
9/30 (2010)
JCR quartile
2 (2010)
ISSN
1434-6621
DOI
10.1515/CCLM.2010.250
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1181498
handle
http://hdl.handle.net/1854/LU-1181498
date created
2011-03-03 11:09:24
date last changed
2016-12-19 15:45:14
@article{1181498,
  abstract     = {Background: Multiple human and animal studies have reviewed the (non-)actin scavenger functions of vitamin D binding protein (DBP). Recently, we demonstrated the partially lipid bound character of DBP. The purpose of the present study was to explore the link between actin, lipids and DBP in a cohort of patients undergoing cardiac surgery.
Methods: The interplay between DBP, actin and lipids was investigated in a cohort study of 35 Caucasian patients who underwent cardiac surgery. Total and actin-free DBP concentrations were assessed by immunonephelometry and ELISA. Total cholesterol, high-density lipoprotein (HDL) cholesterol, triglycerides, aspartate aminotransferase, alanine aminotransferase, lactate dehydrogenase, creatine kinase (-MB) and C-reactive protein (CRP) were measured using routine methods. Low-density lipoprotein cholesterol was calculated according to the Friedewald formula. The DBP phenotypes and the macromolecular bound DBP fractions were determined by polyacrylamide gel electrophoresis (PAGE) and confirmed by Western blotting.
Results: Using PAGE and Western blotting with an antihuman DBP polyclonal antibody, the actin-bound DBP complex was identified in serum. Following cardiac surgery, total serum DBP concentrations were characterized by a two phased course. This was paralleled by a similar pattern in serum total cholesterol, HDL-cholesterol and triglyceride concentrations. Good correlation was found between total and actin free serum DBP concentrations (r=0.69, p{\textlangle}0.0001). The serum actin free DBP/total DBP ratio remained stable throughout the study period. Although no significant correlation between cumulative CK-MB enzyme release and delta serum total DBP concentration was observed (p=NS), the latter value correlated significantly with delta serum triglyceride concentrations (r=0.37, p{\textlangle}0.05).
Conclusions: The lipid bound character is an underestimated property of DBP in the extracellular actin-scavenger system. Clin Chem Lab Med 2010;48:1345-50.},
  author       = {Speeckaert, Marijn and Wehlou, Charline and De Somer, Filip and Speeckaert, Reinhart and Van Nooten, Guido and Delanghe, Joris},
  issn         = {1434-6621},
  journal      = {CLINICAL CHEMISTRY AND LABORATORY MEDICINE},
  keyword      = {vitamin D binding protein,lipids,cardiac surgery,actin scavenging,CATALYTIC ACTIVITY CONCENTRATIONS,GROUP-SPECIFIC COMPONENT,GC-GLOBULIN,CARDIOPULMONARY BYPASS,CHOLESTEROL,37-DEGREES-C,ENZYMES,ACTIN,AMINOTRANSFERASE,COMPLEXES},
  language     = {eng},
  number       = {9},
  pages        = {1345--1350},
  title        = {Evolution of vitamin D binding protein concentration in sera from cardiac surgery patients is determined by triglyceridemia},
  url          = {http://dx.doi.org/10.1515/CCLM.2010.250},
  volume       = {48},
  year         = {2010},
}

Chicago
Speeckaert, Marijn, Charline Wehlou, Filip De Somer, Reinhart Speeckaert, Guido Van Nooten, and Joris Delanghe. 2010. “Evolution of Vitamin D Binding Protein Concentration in Sera from Cardiac Surgery Patients Is Determined by Triglyceridemia.” Clinical Chemistry and Laboratory Medicine 48 (9): 1345–1350.
APA
Speeckaert, M., Wehlou, C., De Somer, F., Speeckaert, R., Van Nooten, G., & Delanghe, J. (2010). Evolution of vitamin D binding protein concentration in sera from cardiac surgery patients is determined by triglyceridemia. CLINICAL CHEMISTRY AND LABORATORY MEDICINE, 48(9), 1345–1350.
Vancouver
1.
Speeckaert M, Wehlou C, De Somer F, Speeckaert R, Van Nooten G, Delanghe J. Evolution of vitamin D binding protein concentration in sera from cardiac surgery patients is determined by triglyceridemia. CLINICAL CHEMISTRY AND LABORATORY MEDICINE. 2010;48(9):1345–50.
MLA
Speeckaert, Marijn, Charline Wehlou, Filip De Somer, et al. “Evolution of Vitamin D Binding Protein Concentration in Sera from Cardiac Surgery Patients Is Determined by Triglyceridemia.” CLINICAL CHEMISTRY AND LABORATORY MEDICINE 48.9 (2010): 1345–1350. Print.