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High-throughput profiling of the serum N-glycome on capillary electrophoresis microfluidics systems: toward clinical implementation of glycoHepatoTest

(2010) ANALYTICAL CHEMISTRY. 82(17). p.7408-7415
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Abstract
We developed a 3 h procedure for preparing serum N-glycans and labeling them with 8-aminopyrene-1,3,6-trisulfonic acid (APTS) by sequential addition of reagents to the serum and incubation in a polymerase chain reaction (PCR) thermocycler. Moreover, we succeeded in analyzing these samples by capillary electrophoresis on three commercial microfluidics-based platforms: the MCE-202 MultiNA, the 2100 Bioanalyzer, and a modified prototype of the eGene system which were originally designed for nucleic acid separation and detection. Although these instruments use short separation channels, our technical improvements made it possible to reliably measure the N-glycans constituting GlycoHepatoTest. This test comprises a panel of biomarkers that allows follow-up of liver fibrosis patients starting from the early stage. In this way and for the first time, we demonstrate a clinical glycomics assay on an affordable, robust platform so that clinical chemistry laboratories can exploit glycomics in the diagnosis and monitoring of chronic liver disease. Another potential application is the rapid screening of the N-glycosylation of recombinant glycoproteins produced for pharmaceutical use.
Keywords
GEL-ELECTROPHORESIS, PROTEIN GLYCOMICS, CHRONIC HEPATITIS-C, LIVER-BIOPSY, BIOMARKERS, CIRRHOSIS, FIBROSIS

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Chicago
Vanderschaeghe, Dieter, Akos Szekrenyes, Christian Wenz, Marcus Gassmann, Natasha Naik, Maggie Bynum, Hongfeng Yin, Joris Delanghe, Andras Guttman, and Nico Callewaert. 2010. “High-throughput Profiling of the Serum N-glycome on Capillary Electrophoresis Microfluidics Systems: Toward Clinical Implementation of glycoHepatoTest.” Analytical Chemistry 82 (17): 7408–7415.
APA
Vanderschaeghe, D., Szekrenyes, A., Wenz, C., Gassmann, M., Naik, N., Bynum, M., Yin, H., et al. (2010). High-throughput profiling of the serum N-glycome on capillary electrophoresis microfluidics systems: toward clinical implementation of glycoHepatoTest. ANALYTICAL CHEMISTRY, 82(17), 7408–7415.
Vancouver
1.
Vanderschaeghe D, Szekrenyes A, Wenz C, Gassmann M, Naik N, Bynum M, et al. High-throughput profiling of the serum N-glycome on capillary electrophoresis microfluidics systems: toward clinical implementation of glycoHepatoTest. ANALYTICAL CHEMISTRY. 2010;82(17):7408–15.
MLA
Vanderschaeghe, Dieter, Akos Szekrenyes, Christian Wenz, et al. “High-throughput Profiling of the Serum N-glycome on Capillary Electrophoresis Microfluidics Systems: Toward Clinical Implementation of glycoHepatoTest.” ANALYTICAL CHEMISTRY 82.17 (2010): 7408–7415. Print.
@article{1181478,
  abstract     = {We developed a 3 h procedure for preparing serum N-glycans and labeling them with 8-aminopyrene-1,3,6-trisulfonic acid (APTS) by sequential addition of reagents to the serum and incubation in a polymerase chain reaction (PCR) thermocycler. Moreover, we succeeded in analyzing these samples by capillary electrophoresis on three commercial microfluidics-based platforms: the MCE-202 MultiNA, the 2100 Bioanalyzer, and a modified prototype of the eGene system which were originally designed for nucleic acid separation and detection. Although these instruments use short separation channels, our technical improvements made it possible to reliably measure the N-glycans constituting GlycoHepatoTest. This test comprises a panel of biomarkers that allows follow-up of liver fibrosis patients starting from the early stage. In this way and for the first time, we demonstrate a clinical glycomics assay on an affordable, robust platform so that clinical chemistry laboratories can exploit glycomics in the diagnosis and monitoring of chronic liver disease. Another potential application is the rapid screening of the N-glycosylation of recombinant glycoproteins produced for pharmaceutical use.},
  author       = {Vanderschaeghe, Dieter and Szekrenyes, Akos and Wenz, Christian and Gassmann, Marcus and Naik, Natasha and Bynum, Maggie and Yin, Hongfeng and Delanghe, Joris and Guttman, Andras and Callewaert, Nico},
  issn         = {0003-2700},
  journal      = {ANALYTICAL CHEMISTRY},
  keyword      = {GEL-ELECTROPHORESIS,PROTEIN GLYCOMICS,CHRONIC HEPATITIS-C,LIVER-BIOPSY,BIOMARKERS,CIRRHOSIS,FIBROSIS},
  language     = {eng},
  number       = {17},
  pages        = {7408--7415},
  title        = {High-throughput profiling of the serum N-glycome on capillary electrophoresis microfluidics systems: toward clinical implementation of glycoHepatoTest},
  url          = {http://dx.doi.org/10.1021/ac101560a},
  volume       = {82},
  year         = {2010},
}

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