Advanced search
1 file | 1.04 MB

The micronucleus assay as a biological dosimeter of in vivo ionising radiation exposure

Anne Vral (UGent) , Michel Fenech and Hubert Thierens (UGent)
(2011) MUTAGENESIS. 26(1). p.11-17
Author
Organization
Abstract
Biological dosimetry, based on the analysis of micronuclei (MN) in the cytokinesis-block micronucleus (CBMN) assay can be used as an alternative method for scoring dicentric chromosomes in the field of radiation protection. Biological dosimetry or Biodosimetry, is mainly performed, in addition to physical dosimetry, with the aim of individual dose assessment. Many studies have shown that the number of radiation-induced MN is strongly correlated with dose and quality of radiation. The CBMN assay has become, in the last years, a thoroughly validated and standardised technique to evaluate in vivo radiation exposure of occupational, medical and accidentally exposed individuals. Compared to the gold standard, the dicentric assay, the CBMN assay has the important advantage of allowing economical, easy and quick analysis. The main disadvantage of the CBMN assay is related to the variable micronucleus ( MN) background frequency, by which only in vivo exposures in excess of 0.2-0.3 Gy X-rays can be detected. In the last years, several improvements have been achieved, with the ultimate goals (i) of further increasing the sensitivity of the CBMN assay for low-dose detection by combining the assay with a fluorescence in situ hybridisation centromere staining technique, (ii) of increasing the specificity of the test for radiation by scoring nucleoplasmic bridges in binucleated cells and (iii) of making the assay optimally suitable for rapid automated analysis of a large number of samples, viz. in case of a large-scale radiation accident. The development of a combined automated MN-centromere scoring procedure remains a challenge for the future, as it will allow systematic biomonitoring of radiation workers exposed to low-dose radiation.
Keywords
PARTIAL-BODY-RADIOTHERAPY, CYTOKINESIS-BLOCKED LYMPHOCYTES, AUTOMATED IMAGE-ANALYSIS, POWER-PLANT WORKERS, CANCER-PATIENTS, CENTROMERE ASSAY, CHROMOSOME-ABERRATIONS, RADIOLOGICAL WORKERS, SCORING PROCEDURE, PERIPHERAL-BLOOD LYMPHOCYTES

Downloads

  • The micronucleus assay as a biological dosimeter of in vivo ionising radiation exposure.pdf
    • full text
    • |
    • open access
    • |
    • PDF
    • |
    • 1.04 MB

Citation

Please use this url to cite or link to this publication:

Chicago
Vral, Anne, Michel Fenech, and Hubert Thierens. 2011. “The Micronucleus Assay as a Biological Dosimeter of in Vivo Ionising Radiation Exposure.” Mutagenesis 26 (1): 11–17.
APA
Vral, A., Fenech, M., & Thierens, H. (2011). The micronucleus assay as a biological dosimeter of in vivo ionising radiation exposure. MUTAGENESIS, 26(1), 11–17.
Vancouver
1.
Vral A, Fenech M, Thierens H. The micronucleus assay as a biological dosimeter of in vivo ionising radiation exposure. MUTAGENESIS. 2011;26(1):11–7.
MLA
Vral, Anne, Michel Fenech, and Hubert Thierens. “The Micronucleus Assay as a Biological Dosimeter of in Vivo Ionising Radiation Exposure.” MUTAGENESIS 26.1 (2011): 11–17. Print.
@article{1180569,
  abstract     = {Biological dosimetry, based on the analysis of micronuclei (MN) in the cytokinesis-block micronucleus (CBMN) assay can be used as an alternative method for scoring dicentric chromosomes in the field of radiation protection. Biological dosimetry or Biodosimetry, is mainly performed, in addition to physical dosimetry, with the aim of individual dose assessment. Many studies have shown that the number of radiation-induced MN is strongly correlated with dose and quality of radiation. The CBMN assay has become, in the last years, a thoroughly validated and standardised technique to evaluate in vivo radiation exposure of occupational, medical and accidentally exposed individuals. Compared to the gold standard, the dicentric assay, the CBMN assay has the important advantage of allowing economical, easy and quick analysis. The main disadvantage of the CBMN assay is related to the variable micronucleus ( MN) background frequency, by which only in vivo exposures in excess of 0.2-0.3 Gy X-rays can be detected.
In the last years, several improvements have been achieved, with the ultimate goals (i) of further increasing the sensitivity of the CBMN assay for low-dose detection by combining the assay with a fluorescence in situ hybridisation centromere staining technique, (ii) of increasing the specificity of the test for radiation by scoring nucleoplasmic bridges in binucleated cells and (iii) of making the assay optimally suitable for rapid automated analysis of a large number of samples, viz. in case of a large-scale radiation accident. The development of a combined automated MN-centromere scoring procedure remains a challenge for the future, as it will allow systematic biomonitoring of radiation workers exposed to low-dose radiation.},
  author       = {Vral, Anne and Fenech, Michel and Thierens, Hubert},
  issn         = {0267-8357},
  journal      = {MUTAGENESIS},
  keyword      = {PARTIAL-BODY-RADIOTHERAPY,CYTOKINESIS-BLOCKED LYMPHOCYTES,AUTOMATED IMAGE-ANALYSIS,POWER-PLANT WORKERS,CANCER-PATIENTS,CENTROMERE ASSAY,CHROMOSOME-ABERRATIONS,RADIOLOGICAL WORKERS,SCORING PROCEDURE,PERIPHERAL-BLOOD LYMPHOCYTES},
  language     = {eng},
  number       = {1},
  pages        = {11--17},
  title        = {The micronucleus assay as a biological dosimeter of in vivo ionising radiation exposure},
  url          = {http://dx.doi.org/10.1093/mutage/geq078},
  volume       = {26},
  year         = {2011},
}

Altmetric
View in Altmetric
Web of Science
Times cited: