Ghent University Academic Bibliography

Advanced

The micronucleus assay as a biological dosimeter of in vivo ionising radiation exposure

Anne Vral UGent, Michel Fenech and Hubert Thierens UGent (2011) MUTAGENESIS. 26(1). p.11-17
abstract
Biological dosimetry, based on the analysis of micronuclei (MN) in the cytokinesis-block micronucleus (CBMN) assay can be used as an alternative method for scoring dicentric chromosomes in the field of radiation protection. Biological dosimetry or Biodosimetry, is mainly performed, in addition to physical dosimetry, with the aim of individual dose assessment. Many studies have shown that the number of radiation-induced MN is strongly correlated with dose and quality of radiation. The CBMN assay has become, in the last years, a thoroughly validated and standardised technique to evaluate in vivo radiation exposure of occupational, medical and accidentally exposed individuals. Compared to the gold standard, the dicentric assay, the CBMN assay has the important advantage of allowing economical, easy and quick analysis. The main disadvantage of the CBMN assay is related to the variable micronucleus ( MN) background frequency, by which only in vivo exposures in excess of 0.2-0.3 Gy X-rays can be detected. In the last years, several improvements have been achieved, with the ultimate goals (i) of further increasing the sensitivity of the CBMN assay for low-dose detection by combining the assay with a fluorescence in situ hybridisation centromere staining technique, (ii) of increasing the specificity of the test for radiation by scoring nucleoplasmic bridges in binucleated cells and (iii) of making the assay optimally suitable for rapid automated analysis of a large number of samples, viz. in case of a large-scale radiation accident. The development of a combined automated MN-centromere scoring procedure remains a challenge for the future, as it will allow systematic biomonitoring of radiation workers exposed to low-dose radiation.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (review)
publication status
published
subject
keyword
PARTIAL-BODY-RADIOTHERAPY, CYTOKINESIS-BLOCKED LYMPHOCYTES, AUTOMATED IMAGE-ANALYSIS, POWER-PLANT WORKERS, CANCER-PATIENTS, CENTROMERE ASSAY, CHROMOSOME-ABERRATIONS, RADIOLOGICAL WORKERS, SCORING PROCEDURE, PERIPHERAL-BLOOD LYMPHOCYTES
journal title
MUTAGENESIS
Mutagenesis
volume
26
issue
1
pages
11 - 17
Web of Science type
Review
Web of Science id
000285627700003
JCR category
TOXICOLOGY
JCR impact factor
3.183 (2011)
JCR rank
21/83 (2011)
JCR quartile
2 (2011)
ISSN
0267-8357
DOI
10.1093/mutage/geq078
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1180569
handle
http://hdl.handle.net/1854/LU-1180569
date created
2011-03-02 13:55:51
date last changed
2012-01-01 00:30:35
@article{1180569,
  abstract     = {Biological dosimetry, based on the analysis of micronuclei (MN) in the cytokinesis-block micronucleus (CBMN) assay can be used as an alternative method for scoring dicentric chromosomes in the field of radiation protection. Biological dosimetry or Biodosimetry, is mainly performed, in addition to physical dosimetry, with the aim of individual dose assessment. Many studies have shown that the number of radiation-induced MN is strongly correlated with dose and quality of radiation. The CBMN assay has become, in the last years, a thoroughly validated and standardised technique to evaluate in vivo radiation exposure of occupational, medical and accidentally exposed individuals. Compared to the gold standard, the dicentric assay, the CBMN assay has the important advantage of allowing economical, easy and quick analysis. The main disadvantage of the CBMN assay is related to the variable micronucleus ( MN) background frequency, by which only in vivo exposures in excess of 0.2-0.3 Gy X-rays can be detected.
In the last years, several improvements have been achieved, with the ultimate goals (i) of further increasing the sensitivity of the CBMN assay for low-dose detection by combining the assay with a fluorescence in situ hybridisation centromere staining technique, (ii) of increasing the specificity of the test for radiation by scoring nucleoplasmic bridges in binucleated cells and (iii) of making the assay optimally suitable for rapid automated analysis of a large number of samples, viz. in case of a large-scale radiation accident. The development of a combined automated MN-centromere scoring procedure remains a challenge for the future, as it will allow systematic biomonitoring of radiation workers exposed to low-dose radiation.},
  author       = {Vral, Anne and Fenech, Michel and Thierens, Hubert},
  issn         = {0267-8357},
  journal      = {MUTAGENESIS},
  keyword      = {PARTIAL-BODY-RADIOTHERAPY,CYTOKINESIS-BLOCKED LYMPHOCYTES,AUTOMATED IMAGE-ANALYSIS,POWER-PLANT WORKERS,CANCER-PATIENTS,CENTROMERE ASSAY,CHROMOSOME-ABERRATIONS,RADIOLOGICAL WORKERS,SCORING PROCEDURE,PERIPHERAL-BLOOD LYMPHOCYTES},
  language     = {eng},
  number       = {1},
  pages        = {11--17},
  title        = {The micronucleus assay as a biological dosimeter of in vivo ionising radiation exposure},
  url          = {http://dx.doi.org/10.1093/mutage/geq078},
  volume       = {26},
  year         = {2011},
}

Chicago
Vral, Anne, Michel Fenech, and Hubert Thierens. 2011. “The Micronucleus Assay as a Biological Dosimeter of in Vivo Ionising Radiation Exposure.” Mutagenesis 26 (1): 11–17.
APA
Vral, A., Fenech, M., & Thierens, H. (2011). The micronucleus assay as a biological dosimeter of in vivo ionising radiation exposure. MUTAGENESIS, 26(1), 11–17.
Vancouver
1.
Vral A, Fenech M, Thierens H. The micronucleus assay as a biological dosimeter of in vivo ionising radiation exposure. MUTAGENESIS. 2011;26(1):11–7.
MLA
Vral, Anne, Michel Fenech, and Hubert Thierens. “The Micronucleus Assay as a Biological Dosimeter of in Vivo Ionising Radiation Exposure.” MUTAGENESIS 26.1 (2011): 11–17. Print.