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Bacillus thermolactis sp. nov., isolated from dairy farms and emended description of Bacillus thermoamylovorans

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Abstract
A polyphasic taxonomic study was performed on 22 thermotolerant, aerobic, endospore-forming bacteria from dairy environments. Seventeen isolates were retrieved from raw milk, one from a filter cloth and four were from grass, straw and milking equipment. These four isolates (R-6546, R-7499, R-7764 and R-7440) were identified as Bacillus thermoamylovorans based on DNA/DNA hybridisations (values above 70% with LMG 18084T, the type strain of Bacillus thermoamylovorans) but showed discrepancies in characteristics with the original species description, so an emended description of this species is given. According to 16S rRNA gene sequence analysis and DNA/DNA hybridisation experiments, the remaining 18 isolates (R-6488T, R-28193, R-6491, R-6492, R-7336, R-33367, R-6486, R-6770, R-31288, R-28160, R-26358, R-7632, R-26955, R-26950, R-33520, R-6484, R-26954 and R-7165) represented one single species, most closely related to Bacillus thermoamylovorans (93,9% 16S rRNA gene sequence similarity), for which the name Bacillus thermolactis is proposed. Cells were Gram-positive, facultatively anaerobic, endospore-forming rods that grew optimally at 40-50°C. The cell wall peptidoglycan type of strain R-6488T, the proposed type strain, was A1{gamma} based on meso-diaminopimelic acid. Major fatty acids of the strains were C16:0 (27.97%), iso-C16:0 (12.06%) and iso-C15:0 (12.04%). MK-7 was the predominant menaquinone, and major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and some unidentified phospholipids. DNA G + C content was 35.0 mol%. Phenotypic properties allowed discrimination from other thermotolerant Bacillus species and supported the description of the novel Bacillus thermolactis species with strain R-6488T (= LMG 25569T = DSM 23332T) as the proposed type strain.
Keywords
HYBRIDIZATION, LIKELIHOOD, PAENIBACILLUS, IDENTIFICATION, CLASSIFICATION, GEN-NOV, MILK-PRODUCTS, MURAL PAINTINGS, ENDOSPORE-FORMING BACTERIA, DEOXYRIBONUCLEIC-ACID

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Chicago
Coorevits, An, Niall A Logan, Anna Dinsdale, Gillian Halket, Patsy Scheldeman, Marc Heyndrickx, Peter Schumann, Anita Van Landschoot, and Paul De Vos. 2011. “Bacillus Thermolactis Sp. Nov., Isolated from Dairy Farms and Emended Description of Bacillus Thermoamylovorans.” International Journal of Systematic and Evolutionary Microbiology 61 (8): 1954–1961.
APA
Coorevits, A., Logan, N. A., Dinsdale, A., Halket, G., Scheldeman, P., Heyndrickx, M., Schumann, P., et al. (2011). Bacillus thermolactis sp. nov., isolated from dairy farms and emended description of Bacillus thermoamylovorans. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 61(8), 1954–1961.
Vancouver
1.
Coorevits A, Logan NA, Dinsdale A, Halket G, Scheldeman P, Heyndrickx M, et al. Bacillus thermolactis sp. nov., isolated from dairy farms and emended description of Bacillus thermoamylovorans. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY. 2011;61(8):1954–61.
MLA
Coorevits, An, Niall A Logan, Anna Dinsdale, et al. “Bacillus Thermolactis Sp. Nov., Isolated from Dairy Farms and Emended Description of Bacillus Thermoamylovorans.” INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 61.8 (2011): 1954–1961. Print.
@article{1180022,
  abstract     = {A polyphasic taxonomic study was performed on 22 thermotolerant, aerobic, endospore-forming bacteria from dairy environments. Seventeen isolates were retrieved from raw milk, one from a filter cloth and four were from grass, straw and milking equipment. These four isolates (R-6546, R-7499, R-7764 and R-7440) were identified as Bacillus thermoamylovorans based on DNA/DNA hybridisations (values above 70\% with LMG 18084T, the type strain of Bacillus thermoamylovorans) but showed discrepancies in characteristics with the original species description, so an emended description of this species is given. According to 16S rRNA gene sequence analysis and DNA/DNA hybridisation experiments, the remaining 18 isolates (R-6488T, R-28193, R-6491, R-6492, R-7336, R-33367, R-6486, R-6770, R-31288, R-28160, R-26358, R-7632, R-26955, R-26950, R-33520, R-6484, R-26954 and R-7165) represented one single species, most closely related to Bacillus thermoamylovorans (93,9\% 16S rRNA gene sequence similarity), for which the name Bacillus thermolactis is proposed. Cells were Gram-positive, facultatively anaerobic, endospore-forming rods that grew optimally at 40-50{\textdegree}C. The cell wall peptidoglycan type of strain R-6488T, the proposed type strain, was A1\{gamma\} based on meso-diaminopimelic acid. Major fatty acids of the strains were C16:0 (27.97\%), iso-C16:0 (12.06\%) and iso-C15:0 (12.04\%). MK-7 was the predominant menaquinone, and major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and some unidentified phospholipids. DNA G + C content was 35.0 mol\%. Phenotypic properties allowed discrimination from other thermotolerant Bacillus species and supported the description of the novel Bacillus thermolactis species with strain R-6488T (= LMG 25569T = DSM 23332T) as the proposed type strain.},
  author       = {Coorevits, An and Logan, Niall A and Dinsdale, Anna and Halket, Gillian and Scheldeman, Patsy and Heyndrickx, Marc and Schumann, Peter and Van Landschoot, Anita and De Vos, Paul},
  issn         = {1466-5026},
  journal      = {INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY},
  keyword      = {HYBRIDIZATION,LIKELIHOOD,PAENIBACILLUS,IDENTIFICATION,CLASSIFICATION,GEN-NOV,MILK-PRODUCTS,MURAL PAINTINGS,ENDOSPORE-FORMING BACTERIA,DEOXYRIBONUCLEIC-ACID},
  language     = {eng},
  number       = {8},
  pages        = {1954--1961},
  title        = {Bacillus thermolactis sp. nov., isolated from dairy farms and emended description of Bacillus thermoamylovorans},
  url          = {http://dx.doi.org/10.1099/ijs.0.024240-0},
  volume       = {61},
  year         = {2011},
}

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