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Bacillus thermolactis sp. nov., isolated from dairy farms and emended description of Bacillus thermoamylovorans

An Coorevits, Niall A Logan, Anna Dinsdale, Gillian Halket, Patsy Scheldeman, Marc Heyndrickx UGent, Peter Schumann, Anita Van Landschoot UGent and Paul De Vos UGent (2011) INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY. 61(8). p.1954-1961
abstract
A polyphasic taxonomic study was performed on 22 thermotolerant, aerobic, endospore-forming bacteria from dairy environments. Seventeen isolates were retrieved from raw milk, one from a filter cloth and four were from grass, straw and milking equipment. These four isolates (R-6546, R-7499, R-7764 and R-7440) were identified as Bacillus thermoamylovorans based on DNA/DNA hybridisations (values above 70% with LMG 18084T, the type strain of Bacillus thermoamylovorans) but showed discrepancies in characteristics with the original species description, so an emended description of this species is given. According to 16S rRNA gene sequence analysis and DNA/DNA hybridisation experiments, the remaining 18 isolates (R-6488T, R-28193, R-6491, R-6492, R-7336, R-33367, R-6486, R-6770, R-31288, R-28160, R-26358, R-7632, R-26955, R-26950, R-33520, R-6484, R-26954 and R-7165) represented one single species, most closely related to Bacillus thermoamylovorans (93,9% 16S rRNA gene sequence similarity), for which the name Bacillus thermolactis is proposed. Cells were Gram-positive, facultatively anaerobic, endospore-forming rods that grew optimally at 40-50°C. The cell wall peptidoglycan type of strain R-6488T, the proposed type strain, was A1{gamma} based on meso-diaminopimelic acid. Major fatty acids of the strains were C16:0 (27.97%), iso-C16:0 (12.06%) and iso-C15:0 (12.04%). MK-7 was the predominant menaquinone, and major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and some unidentified phospholipids. DNA G + C content was 35.0 mol%. Phenotypic properties allowed discrimination from other thermotolerant Bacillus species and supported the description of the novel Bacillus thermolactis species with strain R-6488T (= LMG 25569T = DSM 23332T) as the proposed type strain.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
HYBRIDIZATION, LIKELIHOOD, PAENIBACILLUS, IDENTIFICATION, CLASSIFICATION, GEN-NOV, MILK-PRODUCTS, MURAL PAINTINGS, ENDOSPORE-FORMING BACTERIA, DEOXYRIBONUCLEIC-ACID
journal title
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
Int. J. Syst. Evol. Microbiol.
volume
61
issue
8
pages
1954 - 1961
Web of Science type
Article
Web of Science id
000294514500029
JCR category
MICROBIOLOGY
JCR impact factor
2.268 (2011)
JCR rank
63/111 (2011)
JCR quartile
3 (2011)
ISSN
1466-5026
DOI
10.1099/ijs.0.024240-0
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1180022
handle
http://hdl.handle.net/1854/LU-1180022
date created
2011-03-02 09:04:18
date last changed
2016-12-19 15:46:42
@article{1180022,
  abstract     = {A polyphasic taxonomic study was performed on 22 thermotolerant, aerobic, endospore-forming bacteria from dairy environments. Seventeen isolates were retrieved from raw milk, one from a filter cloth and four were from grass, straw and milking equipment. These four isolates (R-6546, R-7499, R-7764 and R-7440) were identified as Bacillus thermoamylovorans based on DNA/DNA hybridisations (values above 70\% with LMG 18084T, the type strain of Bacillus thermoamylovorans) but showed discrepancies in characteristics with the original species description, so an emended description of this species is given. According to 16S rRNA gene sequence analysis and DNA/DNA hybridisation experiments, the remaining 18 isolates (R-6488T, R-28193, R-6491, R-6492, R-7336, R-33367, R-6486, R-6770, R-31288, R-28160, R-26358, R-7632, R-26955, R-26950, R-33520, R-6484, R-26954 and R-7165) represented one single species, most closely related to Bacillus thermoamylovorans (93,9\% 16S rRNA gene sequence similarity), for which the name Bacillus thermolactis is proposed. Cells were Gram-positive, facultatively anaerobic, endospore-forming rods that grew optimally at 40-50{\textdegree}C. The cell wall peptidoglycan type of strain R-6488T, the proposed type strain, was A1\{gamma\} based on meso-diaminopimelic acid. Major fatty acids of the strains were C16:0 (27.97\%), iso-C16:0 (12.06\%) and iso-C15:0 (12.04\%). MK-7 was the predominant menaquinone, and major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and some unidentified phospholipids. DNA G + C content was 35.0 mol\%. Phenotypic properties allowed discrimination from other thermotolerant Bacillus species and supported the description of the novel Bacillus thermolactis species with strain R-6488T (= LMG 25569T = DSM 23332T) as the proposed type strain.},
  author       = {Coorevits, An and Logan, Niall A and Dinsdale, Anna and Halket, Gillian and Scheldeman, Patsy and Heyndrickx, Marc and Schumann, Peter and Van Landschoot, Anita and De Vos, Paul},
  issn         = {1466-5026},
  journal      = {INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY},
  keyword      = {HYBRIDIZATION,LIKELIHOOD,PAENIBACILLUS,IDENTIFICATION,CLASSIFICATION,GEN-NOV,MILK-PRODUCTS,MURAL PAINTINGS,ENDOSPORE-FORMING BACTERIA,DEOXYRIBONUCLEIC-ACID},
  language     = {eng},
  number       = {8},
  pages        = {1954--1961},
  title        = {Bacillus thermolactis sp. nov., isolated from dairy farms and emended description of Bacillus thermoamylovorans},
  url          = {http://dx.doi.org/10.1099/ijs.0.024240-0},
  volume       = {61},
  year         = {2011},
}

Chicago
Coorevits, An, Niall A Logan, Anna Dinsdale, Gillian Halket, Patsy Scheldeman, Marc Heyndrickx, Peter Schumann, Anita Van Landschoot, and Paul De Vos. 2011. “Bacillus Thermolactis Sp. Nov., Isolated from Dairy Farms and Emended Description of Bacillus Thermoamylovorans.” International Journal of Systematic and Evolutionary Microbiology 61 (8): 1954–1961.
APA
Coorevits, A., Logan, N. A., Dinsdale, A., Halket, G., Scheldeman, P., Heyndrickx, M., Schumann, P., et al. (2011). Bacillus thermolactis sp. nov., isolated from dairy farms and emended description of Bacillus thermoamylovorans. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 61(8), 1954–1961.
Vancouver
1.
Coorevits A, Logan NA, Dinsdale A, Halket G, Scheldeman P, Heyndrickx M, et al. Bacillus thermolactis sp. nov., isolated from dairy farms and emended description of Bacillus thermoamylovorans. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY. 2011;61(8):1954–61.
MLA
Coorevits, An, Niall A Logan, Anna Dinsdale, et al. “Bacillus Thermolactis Sp. Nov., Isolated from Dairy Farms and Emended Description of Bacillus Thermoamylovorans.” INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 61.8 (2011): 1954–1961. Print.