Ghent University Academic Bibliography

Advanced

MAGUKs, scaffolding proteins at cell junctions, are substrates of different proteases during apoptosis

S Ivanova, U Gregorc, N Vidergar, R Javier, DS Bredt, Peter Vandenabeele UGent, J Pardo, MM Simon, V Turk, L Banks, et al. (2011) CELL DEATH & DISEASE. 2.
abstract
A major feature of apoptotic cell death is gross structural changes, one of which is the loss of cell-cell contacts. The caspases, executioners of apoptosis, were shown to cleave several proteins involved in the formation of cell junctions. The membrane-associated guanylate kinases (MAGUKs), which are typically associated with cell junctions, have a major role in the organization of protein-protein complexes at plasma membranes and are therefore potentially important caspase targets during apoptosis. We report here that MAGUKs are cleaved and/or degraded by executioner caspases, granzyme B and several cysteine cathepsins in vitro. When apoptosis was induced by UV-irradiation and staurosporine in different epithelial cell lines, caspases were found to efficiently cleave MAGUKs in these cell models, as the cleavages could be prevented by a pan-caspase inhibitor N-benzyloxycarbonyl-Val-Ala-Asp(OMe) fluoromethylketone. Using a selective lysosomal disrupting agent L-leucyl-L-leucine methyl ester, which induces apoptosis through the lysosomal pathway, it was further shown that MAGUKs are also cleaved by the cathepsins in HaCaT and CaCo-2 cells. Immunohistological data showed rapid loss of MAGUKs at the sites of cell-cell contacts, preceding actual cell detachment, suggesting that cleavage of MAGUKs is an important step in fast and efficient cell detachment.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
MAGUK, apoptosis, caspase, cathepsin, cell junctions, LYSOSOMAL CYSTEINE PROTEASES, LARGE TUMOR-SUPPRESSOR, TIGHT JUNCTION, CASPASE ACTIVITY, BETA-CATENIN, GRANZYME-B, CLEAVAGE, DEATH, TARGET, LOCALIZATION
journal title
CELL DEATH & DISEASE
Cell Death Dis.
volume
2
article number
e116
pages
11 pages
Web of Science type
Article
Web of Science id
000286621400005
JCR category
CELL BIOLOGY
JCR impact factor
5.333 (2011)
JCR rank
45/178 (2011)
JCR quartile
2 (2011)
ISSN
2041-4889
DOI
10.1038/cddis.2010.92
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1176638
handle
http://hdl.handle.net/1854/LU-1176638
date created
2011-02-28 16:20:10
date last changed
2016-12-21 15:42:32
@article{1176638,
  abstract     = {A major feature of apoptotic cell death is gross structural changes, one of which is the loss of cell-cell contacts. The caspases, executioners of apoptosis, were shown to cleave several proteins involved in the formation of cell junctions. The membrane-associated guanylate kinases (MAGUKs), which are typically associated with cell junctions, have a major role in the organization of protein-protein complexes at plasma membranes and are therefore potentially important caspase targets during apoptosis. We report here that MAGUKs are cleaved and/or degraded by executioner caspases, granzyme B and several cysteine cathepsins in vitro. When apoptosis was induced by UV-irradiation and staurosporine in different epithelial cell lines, caspases were found to efficiently cleave MAGUKs in these cell models, as the cleavages could be prevented by a pan-caspase inhibitor N-benzyloxycarbonyl-Val-Ala-Asp(OMe) fluoromethylketone. Using a selective lysosomal disrupting agent L-leucyl-L-leucine methyl ester, which induces apoptosis through the lysosomal pathway, it was further shown that MAGUKs are also cleaved by the cathepsins in HaCaT and CaCo-2 cells. Immunohistological data showed rapid loss of MAGUKs at the sites of cell-cell contacts, preceding actual cell detachment, suggesting that cleavage of MAGUKs is an important step in fast and efficient cell detachment.},
  articleno    = {e116},
  author       = {Ivanova, S and Gregorc, U and Vidergar, N and Javier, R and Bredt, DS and Vandenabeele, Peter and Pardo, J and Simon, MM and Turk, V and Banks, L and Turk, B},
  issn         = {2041-4889},
  journal      = {CELL DEATH \& DISEASE},
  keyword      = {MAGUK,apoptosis,caspase,cathepsin,cell junctions,LYSOSOMAL CYSTEINE PROTEASES,LARGE TUMOR-SUPPRESSOR,TIGHT JUNCTION,CASPASE ACTIVITY,BETA-CATENIN,GRANZYME-B,CLEAVAGE,DEATH,TARGET,LOCALIZATION},
  language     = {eng},
  pages        = {11},
  title        = {MAGUKs, scaffolding proteins at cell junctions, are substrates of different proteases during apoptosis},
  url          = {http://dx.doi.org/10.1038/cddis.2010.92},
  volume       = {2},
  year         = {2011},
}

Chicago
Ivanova, S, U Gregorc, N Vidergar, R Javier, DS Bredt, Peter Vandenabeele, J Pardo, et al. 2011. “MAGUKs, Scaffolding Proteins at Cell Junctions, Are Substrates of Different Proteases During Apoptosis.” Cell Death & Disease 2.
APA
Ivanova, S., Gregorc, U., Vidergar, N., Javier, R., Bredt, D., Vandenabeele, P., Pardo, J., et al. (2011). MAGUKs, scaffolding proteins at cell junctions, are substrates of different proteases during apoptosis. CELL DEATH & DISEASE, 2.
Vancouver
1.
Ivanova S, Gregorc U, Vidergar N, Javier R, Bredt D, Vandenabeele P, et al. MAGUKs, scaffolding proteins at cell junctions, are substrates of different proteases during apoptosis. CELL DEATH & DISEASE. 2011;2.
MLA
Ivanova, S, U Gregorc, N Vidergar, et al. “MAGUKs, Scaffolding Proteins at Cell Junctions, Are Substrates of Different Proteases During Apoptosis.” CELL DEATH & DISEASE 2 (2011): n. pag. Print.