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MicroRNA miR-885-5p targets CDK2 and MCM5, activates p53 and inhibits proliferation and survival

(2011) CELL DEATH AND DIFFERENTIATION. 18(6). p.974-984
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Bioinformatics: from nucleotids to networks (N2N)
Abstract
Several microRNA (miRNA) loci are found within genomic regions frequently deleted in primary neuroblastoma, including miR-885-5p at 3p25.3. In this study, we demonstrate that miR-885-5p is downregulated on loss of 3p25.3 region in neuroblastoma. Experimentally enforced miR-885-5p expression in neuroblastoma cell lines inhibits proliferation triggering cell cycle arrest, senescence and/or apoptosis. miR-885-5p leads to the accumulation of p53 protein and activates the p53 pathway, resulting in upregulation of p53 targets. Enforced miR-885-5p expression consistently leads to downregulation of cyclin-dependent kinase (CDK2) and mini-chromosome maintenance protein (MCM5). Both genes are targeted by miR-885-5p via predicted binding sites within the 3'-untranslated regions (UTRs) of CDK2 and MCM5. Transcript profiling after miR-885-5p introduction in neuroblastoma cells reveals alterations in expression of multiple genes, including several p53 target genes and a number of factors involved in p53 pathway activity. Taken together, these data provide evidence that miR-885-5p has a tumor suppressive role in neuroblastoma interfering with cell cycle progression and cell survival.
Keywords
CLEAVAGE, AMPLIFICATION, CANCER, miRNA, 3p25.3, p53 stabilization, senescence, NEUROBLASTOMA-CELLS, GENE-EXPRESSION, TUMOR-SUPPRESSOR, DNA-DAMAGE, REGIONS, APOPTOSIS, SITES

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Citation

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Chicago
Afanasyeva, EA, Pieter Mestdagh, Candy Kumps, Jo Vandesompele, V Ehemann, J Theissen, Mark Zapatka, et al. 2011. “MicroRNA miR-885-5p Targets CDK2 and MCM5, Activates P53 and Inhibits Proliferation and Survival.” Cell Death and Differentiation 18 (6): 974–984.
APA
Afanasyeva, E., Mestdagh, P., Kumps, C., Vandesompele, J., Ehemann, V., Theissen, J., Zapatka, M., et al. (2011). MicroRNA miR-885-5p targets CDK2 and MCM5, activates p53 and inhibits proliferation and survival. CELL DEATH AND DIFFERENTIATION, 18(6), 974–984.
Vancouver
1.
Afanasyeva E, Mestdagh P, Kumps C, Vandesompele J, Ehemann V, Theissen J, et al. MicroRNA miR-885-5p targets CDK2 and MCM5, activates p53 and inhibits proliferation and survival. CELL DEATH AND DIFFERENTIATION. 2011;18(6):974–84.
MLA
Afanasyeva, EA, Pieter Mestdagh, Candy Kumps, et al. “MicroRNA miR-885-5p Targets CDK2 and MCM5, Activates P53 and Inhibits Proliferation and Survival.” CELL DEATH AND DIFFERENTIATION 18.6 (2011): 974–984. Print.
@article{1176506,
  abstract     = {Several microRNA (miRNA) loci are found within genomic regions frequently deleted in primary neuroblastoma, including miR-885-5p at 3p25.3. In this study, we demonstrate that miR-885-5p is downregulated on loss of 3p25.3 region in neuroblastoma. Experimentally enforced miR-885-5p expression in neuroblastoma cell lines inhibits proliferation triggering cell cycle arrest, senescence and/or apoptosis. miR-885-5p leads to the accumulation of p53 protein and activates the p53 pathway, resulting in upregulation of p53 targets. Enforced miR-885-5p expression consistently leads to downregulation of cyclin-dependent kinase (CDK2) and mini-chromosome maintenance protein (MCM5). Both genes are targeted by miR-885-5p via predicted binding sites within the 3'-untranslated regions (UTRs) of CDK2 and MCM5. Transcript profiling after miR-885-5p introduction in neuroblastoma cells reveals alterations in expression of multiple genes, including several p53 target genes and a number of factors involved in p53 pathway activity. Taken together, these data provide evidence that miR-885-5p has a tumor suppressive role in neuroblastoma interfering with cell cycle progression and cell survival.},
  author       = {Afanasyeva, EA and Mestdagh, Pieter and Kumps, Candy and Vandesompele, Jo and Ehemann, V and Theissen, J and Zapatka, Mark and Brors, Benedikt and Savelyeva, L and Sagulenko, V and Schwab, Manfred and Speleman, Franki and Westermann, Frank},
  issn         = {1350-9047},
  journal      = {CELL DEATH AND DIFFERENTIATION},
  keyword      = {CLEAVAGE,AMPLIFICATION,CANCER,miRNA,3p25.3,p53 stabilization,senescence,NEUROBLASTOMA-CELLS,GENE-EXPRESSION,TUMOR-SUPPRESSOR,DNA-DAMAGE,REGIONS,APOPTOSIS,SITES},
  language     = {eng},
  number       = {6},
  pages        = {974--984},
  title        = {MicroRNA miR-885-5p targets CDK2 and MCM5, activates p53 and inhibits proliferation and survival},
  url          = {http://dx.doi.org/10.1038/cdd.2010.164},
  volume       = {18},
  year         = {2011},
}

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