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MicroRNA miR-885-5p targets CDK2 and MCM5, activates p53 and inhibits proliferation and survival

(2011) CELL DEATH AND DIFFERENTIATION. 18(6). p.974-984
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Bioinformatics: from nucleotids to networks (N2N)
Abstract
Several microRNA (miRNA) loci are found within genomic regions frequently deleted in primary neuroblastoma, including miR-885-5p at 3p25.3. In this study, we demonstrate that miR-885-5p is downregulated on loss of 3p25.3 region in neuroblastoma. Experimentally enforced miR-885-5p expression in neuroblastoma cell lines inhibits proliferation triggering cell cycle arrest, senescence and/or apoptosis. miR-885-5p leads to the accumulation of p53 protein and activates the p53 pathway, resulting in upregulation of p53 targets. Enforced miR-885-5p expression consistently leads to downregulation of cyclin-dependent kinase (CDK2) and mini-chromosome maintenance protein (MCM5). Both genes are targeted by miR-885-5p via predicted binding sites within the 3'-untranslated regions (UTRs) of CDK2 and MCM5. Transcript profiling after miR-885-5p introduction in neuroblastoma cells reveals alterations in expression of multiple genes, including several p53 target genes and a number of factors involved in p53 pathway activity. Taken together, these data provide evidence that miR-885-5p has a tumor suppressive role in neuroblastoma interfering with cell cycle progression and cell survival.
Keywords
CLEAVAGE, AMPLIFICATION, CANCER, miRNA, 3p25.3, p53 stabilization, senescence, NEUROBLASTOMA-CELLS, GENE-EXPRESSION, TUMOR-SUPPRESSOR, DNA-DAMAGE, REGIONS, APOPTOSIS, SITES

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Citation

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MLA
Afanasyeva, EA, Pieter Mestdagh, Candy Kumps, et al. “MicroRNA miR-885-5p Targets CDK2 and MCM5, Activates P53 and Inhibits Proliferation and Survival.” CELL DEATH AND DIFFERENTIATION 18.6 (2011): 974–984. Print.
APA
Afanasyeva, E., Mestdagh, P., Kumps, C., Vandesompele, J., Ehemann, V., Theissen, J., Zapatka, M., et al. (2011). MicroRNA miR-885-5p targets CDK2 and MCM5, activates p53 and inhibits proliferation and survival. CELL DEATH AND DIFFERENTIATION, 18(6), 974–984.
Chicago author-date
Afanasyeva, EA, Pieter Mestdagh, Candy Kumps, Jo Vandesompele, V Ehemann, J Theissen, Mark Zapatka, et al. 2011. “MicroRNA miR-885-5p Targets CDK2 and MCM5, Activates P53 and Inhibits Proliferation and Survival.” Cell Death and Differentiation 18 (6): 974–984.
Chicago author-date (all authors)
Afanasyeva, EA, Pieter Mestdagh, Candy Kumps, Jo Vandesompele, V Ehemann, J Theissen, Mark Zapatka, Benedikt Brors, L Savelyeva, V Sagulenko, Manfred Schwab, Franki Speleman, and Frank Westermann. 2011. “MicroRNA miR-885-5p Targets CDK2 and MCM5, Activates P53 and Inhibits Proliferation and Survival.” Cell Death and Differentiation 18 (6): 974–984.
Vancouver
1.
Afanasyeva E, Mestdagh P, Kumps C, Vandesompele J, Ehemann V, Theissen J, et al. MicroRNA miR-885-5p targets CDK2 and MCM5, activates p53 and inhibits proliferation and survival. CELL DEATH AND DIFFERENTIATION. 2011;18(6):974–84.
IEEE
[1]
E. Afanasyeva et al., “MicroRNA miR-885-5p targets CDK2 and MCM5, activates p53 and inhibits proliferation and survival,” CELL DEATH AND DIFFERENTIATION, vol. 18, no. 6, pp. 974–984, 2011.
@article{1176506,
  abstract     = {Several microRNA (miRNA) loci are found within genomic regions frequently deleted in primary neuroblastoma, including miR-885-5p at 3p25.3. In this study, we demonstrate that miR-885-5p is downregulated on loss of 3p25.3 region in neuroblastoma. Experimentally enforced miR-885-5p expression in neuroblastoma cell lines inhibits proliferation triggering cell cycle arrest, senescence and/or apoptosis. miR-885-5p leads to the accumulation of p53 protein and activates the p53 pathway, resulting in upregulation of p53 targets. Enforced miR-885-5p expression consistently leads to downregulation of cyclin-dependent kinase (CDK2) and mini-chromosome maintenance protein (MCM5). Both genes are targeted by miR-885-5p via predicted binding sites within the 3'-untranslated regions (UTRs) of CDK2 and MCM5. Transcript profiling after miR-885-5p introduction in neuroblastoma cells reveals alterations in expression of multiple genes, including several p53 target genes and a number of factors involved in p53 pathway activity. Taken together, these data provide evidence that miR-885-5p has a tumor suppressive role in neuroblastoma interfering with cell cycle progression and cell survival.},
  author       = {Afanasyeva, EA and Mestdagh, Pieter and Kumps, Candy and Vandesompele, Jo and Ehemann, V and Theissen, J and Zapatka, Mark and Brors, Benedikt and Savelyeva, L and Sagulenko, V and Schwab, Manfred and Speleman, Franki and Westermann, Frank},
  issn         = {1350-9047},
  journal      = {CELL DEATH AND DIFFERENTIATION},
  keywords     = {CLEAVAGE,AMPLIFICATION,CANCER,miRNA,3p25.3,p53 stabilization,senescence,NEUROBLASTOMA-CELLS,GENE-EXPRESSION,TUMOR-SUPPRESSOR,DNA-DAMAGE,REGIONS,APOPTOSIS,SITES},
  language     = {eng},
  number       = {6},
  pages        = {974--984},
  title        = {MicroRNA miR-885-5p targets CDK2 and MCM5, activates p53 and inhibits proliferation and survival},
  url          = {http://dx.doi.org/10.1038/cdd.2010.164},
  volume       = {18},
  year         = {2011},
}

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