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Inactivation of murine Norovirus-1, coliphage φX174 and Bacteroides fragilis phage B40-8 on surfaces and fresh cut iceberg lettuce by hydrogen peroxide and UV light

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VEG-I-TRADE (Impact of climate change and globalisation on safety of fresh produce – governing a supply chain of uncompromised food sovereignty)
Abstract
In this study, the inactivating properties of liquid hydrogen peroxide (L-H2O2), vaporized hydrogen peroxide (V-H2O2), UV-light and a combination of V-H2O2 and UV-light were tested on murine norovirus 1 (MNV-1), bacteriophages (φX174 and B40-8) as models for human noroviruses. Disinfection of surfaces was examined on stainless steel discs based on EN 13697 : 2001 (12). For the fresh produce decontamination, a mixture of the viruses was inoculated onto shredded iceberg lettuce and treated after overnight incubation at 2°C. According to our results, L-H2O2 [2.1%] was able to inactivate MNV-1 and φX174 on stainless steel discs by approximately 4-log10 within 10 min exposure, whereas for B40-8 15% of L-H2O2 was needed to get a similar reduction in 10 min. Only a marginal reduction (≤1-log10, 5 min exposure) by V-H2O2 [2.52%] was achieved for the tested model viruses. Although in combination with UV-light, a 4-log10 decrease within 5 min treatment was observed on stainless steel discs. Similar trends were obtained for the decontamination of shredded iceberg lettuce but the viral decline was reduced. These results demonstrated that both L-H2O2 and combination of V-H2O2 and UV-light can be used for norovirus inactivation on surfaces; V-H2O2 [2.52%] in combination with UV-light is promising as a decontamination of fresh produce with much less consumption of water and disinfectant.
Keywords
INFECTING BACTEROIDES-FRAGILIS, ACUTE GASTROENTERITIS, FELINE CALICIVIRUS, ESCHERICHIA-COLI, SODIUM-HYPOCHLORITE, PEROXYACETIC ACID, GENETIC DIVERSITY, VIRUS, SURVIVAL, CULTURE, Inactivation, Hydrogen Peroxide, Norovirus

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Citation

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MLA
Li, Dan, Leen Baert, Maarten De Jonghe, et al. “Inactivation of Murine Norovirus-1, Coliphage φX174 and Bacteroides Fragilis Phage B40-8 on Surfaces and Fresh Cut Iceberg Lettuce by Hydrogen Peroxide and UV Light.” APPLIED AND ENVIRONMENTAL MICROBIOLOGY 77.4 (2011): 1399–1404. Print.
APA
Li, Dan, Baert, L., De Jonghe, M., Van Coillie, E., Ryckeboer, J., Devlieghere, F., & Uyttendaele, M. (2011). Inactivation of murine Norovirus-1, coliphage φX174 and Bacteroides fragilis phage B40-8 on surfaces and fresh cut iceberg lettuce by hydrogen peroxide and UV light. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 77(4), 1399–1404.
Chicago author-date
Li, Dan, Leen Baert, Maarten De Jonghe, Els Van Coillie, Jaak Ryckeboer, Frank Devlieghere, and Mieke Uyttendaele. 2011. “Inactivation of Murine Norovirus-1, Coliphage φX174 and Bacteroides Fragilis Phage B40-8 on Surfaces and Fresh Cut Iceberg Lettuce by Hydrogen Peroxide and UV Light.” Applied and Environmental Microbiology 77 (4): 1399–1404.
Chicago author-date (all authors)
Li, Dan, Leen Baert, Maarten De Jonghe, Els Van Coillie, Jaak Ryckeboer, Frank Devlieghere, and Mieke Uyttendaele. 2011. “Inactivation of Murine Norovirus-1, Coliphage φX174 and Bacteroides Fragilis Phage B40-8 on Surfaces and Fresh Cut Iceberg Lettuce by Hydrogen Peroxide and UV Light.” Applied and Environmental Microbiology 77 (4): 1399–1404.
Vancouver
1.
Li D, Baert L, De Jonghe M, Van Coillie E, Ryckeboer J, Devlieghere F, et al. Inactivation of murine Norovirus-1, coliphage φX174 and Bacteroides fragilis phage B40-8 on surfaces and fresh cut iceberg lettuce by hydrogen peroxide and UV light. APPLIED AND ENVIRONMENTAL MICROBIOLOGY. 2011;77(4):1399–404.
IEEE
[1]
D. Li et al., “Inactivation of murine Norovirus-1, coliphage φX174 and Bacteroides fragilis phage B40-8 on surfaces and fresh cut iceberg lettuce by hydrogen peroxide and UV light,” APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 77, no. 4, pp. 1399–1404, 2011.
@article{1175919,
  abstract     = {In this study, the inactivating properties of liquid hydrogen peroxide (L-H2O2), vaporized hydrogen peroxide (V-H2O2), UV-light and a combination of V-H2O2 and UV-light were tested on murine norovirus 1 (MNV-1), bacteriophages (φX174 and B40-8) as models for human noroviruses. Disinfection of surfaces was examined on stainless steel discs based on EN 13697 : 2001 (12). For the fresh produce decontamination, a mixture of the viruses was inoculated onto shredded iceberg lettuce and treated after overnight incubation at 2°C. According to our results, L-H2O2 [2.1%] was able to inactivate MNV-1 and φX174 on stainless steel discs by approximately 4-log10 within 10 min exposure, whereas for B40-8 15% of L-H2O2 was needed to get a similar reduction in 10 min. Only a marginal reduction (≤1-log10, 5 min exposure) by V-H2O2 [2.52%] was achieved for the tested model viruses. Although in combination with UV-light, a 4-log10 decrease within 5 min treatment was observed on stainless steel discs. Similar trends were obtained for the decontamination of shredded iceberg lettuce but the viral decline was reduced. These results demonstrated that both L-H2O2 and combination of V-H2O2 and UV-light can be used for norovirus inactivation on surfaces; V-H2O2 [2.52%] in combination with UV-light is promising as a decontamination of fresh produce with much less consumption of water and disinfectant.},
  author       = {Li, Dan and Baert, Leen and De Jonghe, Maarten and Van Coillie, Els and Ryckeboer, Jaak and Devlieghere, Frank and Uyttendaele, Mieke},
  issn         = {0099-2240},
  journal      = {APPLIED AND ENVIRONMENTAL MICROBIOLOGY},
  keywords     = {INFECTING BACTEROIDES-FRAGILIS,ACUTE GASTROENTERITIS,FELINE CALICIVIRUS,ESCHERICHIA-COLI,SODIUM-HYPOCHLORITE,PEROXYACETIC ACID,GENETIC DIVERSITY,VIRUS,SURVIVAL,CULTURE,Inactivation,Hydrogen Peroxide,Norovirus},
  language     = {eng},
  number       = {4},
  pages        = {1399--1404},
  title        = {Inactivation of murine Norovirus-1, coliphage φX174 and Bacteroides fragilis phage B40-8 on surfaces and fresh cut iceberg lettuce by hydrogen peroxide and UV light},
  url          = {http://dx.doi.org/10.1128/AEM.02131-10},
  volume       = {77},
  year         = {2011},
}

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