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Erwinia oleae sp. nov., isolated from olive knots caused by Pseudomonas savastanoi pv. savastanoi

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Abstract
Three endophytic bacterial isolates were obtained in Italy from olive knots caused by Pseudomonas savastanoi pv. savastanoi. Phenotypic tests in combination with 16S rRNA gene sequence analysis indicated a phylogenetic position of these isolates in the genus Erwinia or Pantoea, and revealed two other strains with highly similar 16S rRNA gene sequences (> 99 %), CECT 5262 and CECT 5264, obtained in Spain from olive knots. Rep-PCR DNA fingerprinting of the five strains from olive knots with BOX, ERIC and REP primers revealed three groups of profiles that were highly similar to each other. Multilocus sequence analysis (MLSA) based on concatenated partial atpD, gyrB, infB and rpoB gene sequences, indicated that the strains constitute a single novel species in the genus Erwinia. The strains showed general phenotypic characteristic of Erwinia, and whole genome DNA-DNA hybridization data confirmed they represent a single novel Erwinia species. The strains showed a DNA G+C base composition ranging from 54.7 to 54.9 mol%. They could be discriminated from the phylogenetically related Erwinia species by their ability to utilise potassium gluconate, L-rhamnose and D-arabitol, but not glycerol, inositol and D-sorbitol. The name Erwinia oleae (type strain DAPP-PG 531T = LMG 25322T = DSM 23398T) is proposed for this new taxon.
Keywords
DEOXYRIBONUCLEIC-ACID, IDENTIFICATION, PEAR TREES, BACTERIA, DNA, PATHOGEN, CLASSIFICATION, PLANTS, STRAINS, HYBRIDIZATION

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Chicago
Moretti, Chiaraluce, Taha Hosni, Katrien Vandemeulebroecke, Carrie Brady, Paul De Vos, Roberto Buonaurio, and Ilse Cleenwerck. 2011. “Erwinia Oleae Sp. Nov., Isolated from Olive Knots Caused by Pseudomonas Savastanoi Pv. Savastanoi.” International Journal of Systematic and Evolutionary Microbiology 61 (11): 2745–2752.
APA
Moretti, C., Hosni, T., Vandemeulebroecke, K., Brady, C., De Vos, P., Buonaurio, R., & Cleenwerck, I. (2011). Erwinia oleae sp. nov., isolated from olive knots caused by Pseudomonas savastanoi pv. savastanoi. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 61(11), 2745–2752.
Vancouver
1.
Moretti C, Hosni T, Vandemeulebroecke K, Brady C, De Vos P, Buonaurio R, et al. Erwinia oleae sp. nov., isolated from olive knots caused by Pseudomonas savastanoi pv. savastanoi. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY. 2011;61(11):2745–52.
MLA
Moretti, Chiaraluce, Taha Hosni, Katrien Vandemeulebroecke, et al. “Erwinia Oleae Sp. Nov., Isolated from Olive Knots Caused by Pseudomonas Savastanoi Pv. Savastanoi.” INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 61.11 (2011): 2745–2752. Print.
@article{1174301,
  abstract     = {Three endophytic bacterial isolates were obtained in Italy from olive knots caused by Pseudomonas savastanoi pv. savastanoi. Phenotypic tests in combination with 16S rRNA gene sequence analysis indicated a phylogenetic position of these isolates in the genus Erwinia or Pantoea, and revealed two other strains with highly similar 16S rRNA gene sequences ({\textrangle} 99 \%), CECT 5262 and CECT 5264, obtained in Spain from olive knots. Rep-PCR DNA fingerprinting of the five strains from olive knots with BOX, ERIC and REP primers revealed three groups of profiles that were highly similar to each other. Multilocus sequence analysis (MLSA) based on concatenated partial atpD, gyrB, infB and rpoB gene sequences, indicated that the strains constitute a single novel species in the genus Erwinia. The strains showed general phenotypic characteristic of Erwinia, and whole genome DNA-DNA hybridization data confirmed they represent a single novel Erwinia species. The strains showed a DNA G+C base composition ranging from 54.7 to 54.9 mol\%. They could be discriminated from the phylogenetically related Erwinia species by their ability to utilise potassium gluconate, L-rhamnose and D-arabitol, but not glycerol, inositol and D-sorbitol. The name Erwinia oleae (type strain DAPP-PG 531T = LMG 25322T = DSM 23398T) is proposed for this new taxon.},
  author       = {Moretti, Chiaraluce and Hosni, Taha and Vandemeulebroecke, Katrien and Brady, Carrie and De Vos, Paul and Buonaurio, Roberto and Cleenwerck, Ilse},
  issn         = {1466-5026},
  journal      = {INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY},
  language     = {eng},
  number       = {11},
  pages        = {2745--2752},
  title        = {Erwinia oleae sp. nov., isolated from olive knots caused by Pseudomonas savastanoi pv. savastanoi},
  url          = {http://dx.doi.org/10.1099/ijs.0.026336-0},
  volume       = {61},
  year         = {2011},
}

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