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DNA fragmentation in canine immature grade 1 cumulus-oocyte complexes

G Lopes, Leen Vandaele (UGent) , Tom Rijsselaere (UGent) , Ann Van Soom (UGent) and A Rocha
(2010) REPRODUCTION IN DOMESTIC ANIMALS. 45(6). p.e275-e281
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Abstract
In this work, we studied the incidence of DNA fragmentation, interpreted as apoptotic changes and assessed by the TUNEL assay, in cumulus cells and oocytes of immature Grade 1 cumulus-oocyte complexes (COCs) obtained from healthy bitches (n = 27) of three age groups: young (1-3 years; n = 13), adult (4-6 years; n = 8) and elderly (7-10 years; n = 6). Age affected (p < 0.05) Grade 1 COCs recovery rates, with young animals yielding more (p < 0.01) Grade 1 COCs than the other two age groups. Conversely, no differences were observed in the incidence of DNA fragmentation (TUNEL-positive) in cumulus cells or oocytes between the three age groups. Overall, more than 80% of Grade 1 COCs presented < 15% of TUNEL-positive cumulus cells and enclosed TUNEL-negative (intact DNA) oocytes. Despite a higher proportion of TUNEL-negative oocytes being found in the germinal vesicle stage, most of the oocytes with nuclear material compatible with meiosis resumption (MR) or with non-identifiable nuclear material (ND) did not present DNA fragmentation. No correlation was observed between DNA fragmentations in oocytes and in cumulus cells. We concluded that the morphological parameters used to classify canine Grade 1 COCs are reliable to select a homogeneous population of COCs with low incidence of DNA fragmentation. Furthermore, these results indicate that DNA fragmentation can only explain a minor proportion of the incidence of MR and degeneration in canine oocytes at collection.
Keywords
IN-VITRO MATURATION, MEIOTIC COMPETENCE, DEVELOPMENTAL COMPETENCE, NUCLEAR MATURATION, OVARIAN FOLLICLE, BOVINE OOCYTES, CELL APOPTOSIS, RECOVERY RATE, BITCH, ATRESIA

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Please use this url to cite or link to this publication:

Chicago
Lopes, G, Leen Vandaele, Tom Rijsselaere, Ann Van Soom, and A Rocha. 2010. “DNA Fragmentation in Canine Immature Grade 1 Cumulus-oocyte Complexes.” Reproduction in Domestic Animals 45 (6): e275–e281.
APA
Lopes, G., Vandaele, L., Rijsselaere, T., Van Soom, A., & Rocha, A. (2010). DNA fragmentation in canine immature grade 1 cumulus-oocyte complexes. REPRODUCTION IN DOMESTIC ANIMALS, 45(6), e275–e281.
Vancouver
1.
Lopes G, Vandaele L, Rijsselaere T, Van Soom A, Rocha A. DNA fragmentation in canine immature grade 1 cumulus-oocyte complexes. REPRODUCTION IN DOMESTIC ANIMALS. 2010;45(6):e275–e281.
MLA
Lopes, G, Leen Vandaele, Tom Rijsselaere, et al. “DNA Fragmentation in Canine Immature Grade 1 Cumulus-oocyte Complexes.” REPRODUCTION IN DOMESTIC ANIMALS 45.6 (2010): e275–e281. Print.
@article{1167691,
  abstract     = {In this work, we studied the incidence of DNA fragmentation, interpreted as apoptotic changes and assessed by the TUNEL assay, in cumulus cells and oocytes of immature Grade 1 cumulus-oocyte complexes (COCs) obtained from healthy bitches (n = 27) of three age groups: young (1-3 years; n = 13), adult (4-6 years; n = 8) and elderly (7-10 years; n = 6). Age affected (p {\textlangle} 0.05) Grade 1 COCs recovery rates, with young animals yielding more (p {\textlangle} 0.01) Grade 1 COCs than the other two age groups. Conversely, no differences were observed in the incidence of DNA fragmentation (TUNEL-positive) in cumulus cells or oocytes between the three age groups. Overall, more than 80\% of Grade 1 COCs presented {\textlangle} 15\% of TUNEL-positive cumulus cells and enclosed TUNEL-negative (intact DNA) oocytes. Despite a higher proportion of TUNEL-negative oocytes being found in the germinal vesicle stage, most of the oocytes with nuclear material compatible with meiosis resumption (MR) or with non-identifiable nuclear material (ND) did not present DNA fragmentation. No correlation was observed between DNA fragmentations in oocytes and in cumulus cells. We concluded that the morphological parameters used to classify canine Grade 1 COCs are reliable to select a homogeneous population of COCs with low incidence of DNA fragmentation. Furthermore, these results indicate that DNA fragmentation can only explain a minor proportion of the incidence of MR and degeneration in canine oocytes at collection.},
  author       = {Lopes, G and Vandaele, Leen and Rijsselaere, Tom and Van Soom, Ann and Rocha, A},
  issn         = {0936-6768},
  journal      = {REPRODUCTION IN DOMESTIC ANIMALS},
  keyword      = {IN-VITRO MATURATION,MEIOTIC COMPETENCE,DEVELOPMENTAL COMPETENCE,NUCLEAR MATURATION,OVARIAN FOLLICLE,BOVINE OOCYTES,CELL APOPTOSIS,RECOVERY RATE,BITCH,ATRESIA},
  language     = {eng},
  number       = {6},
  pages        = {e275--e281},
  title        = {DNA fragmentation in canine immature grade 1 cumulus-oocyte complexes},
  url          = {http://dx.doi.org/10.1111/j.1439-0531.2009.01557.x},
  volume       = {45},
  year         = {2010},
}

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