Advanced search
1 file | 70.39 KB

External quality assessment of multi-analyte chromatographic methods in oral fluid

Author
Organization
Project
DRUID - Driving Under the Influence of Drugs and Medicines
Abstract
Objectives: DRUID (Driving Under the Inflluence of Drugs, Alcohol and Medicines) is a research project funded by the European Commission in which oral fluid is analysed by eleven laboratories. A common collection and analysis methodology has been used: collection with Statsure Saliva Sampler and LC-MS/MS or GC-MS analysis of 22 substances. Four rounds of proficiency testing (PT) were organized between March 2008 and September 2009. Methods: Qualitative results were evaluated using sensitivity and specificity. Quantitative results were evaluated using z-scores and the standard deviation of Horwitz. Results: Specificity was above 99% for all analytes; sensitivity per analyte varied between 81.7 and 100%, 20 out of 22 analytes had a sensitivity >90%. The percentage of satisfactory z-scores increased from 79.4% to 89.2% over the test period. This trend was seen for all drugs, except zopiclone. Results were discussed with participating laboratories and problems were addressed. Conclusions: Because of these corrective actions, DRUID laboratories have a lower variation in results than previously published PT schemes in oral fluid.

Downloads

  • External quality assessment of multi.pdf
    • full text
    • |
    • open access
    • |
    • PDF
    • |
    • 70.39 KB

Citation

Please use this url to cite or link to this publication:

Chicago
Verstraete, Alain, Kristof Pil, and Francis Esposito. 2010. “External Quality Assessment of Multi-analyte Chromatographic Methods in Oral Fluid.” In Tri-annual Meeting of the International Council on Alcohol, Drugs and Traffic Society (ICADTS), Abstracts, 169–169.
APA
Verstraete, Alain, Pil, K., & Esposito, F. (2010). External quality assessment of multi-analyte chromatographic methods in oral fluid. Tri-annual meeting of the International Council on Alcohol, Drugs and Traffic Society (ICADTS), Abstracts (pp. 169–169). Presented at the Tri-annual meeting of the International Council on Alcohol, Drugs and Traffic Society (ICADTS).
Vancouver
1.
Verstraete A, Pil K, Esposito F. External quality assessment of multi-analyte chromatographic methods in oral fluid. Tri-annual meeting of the International Council on Alcohol, Drugs and Traffic Society (ICADTS), Abstracts. 2010. p. 169–169.
MLA
Verstraete, Alain, Kristof Pil, and Francis Esposito. “External Quality Assessment of Multi-analyte Chromatographic Methods in Oral Fluid.” Tri-annual Meeting of the International Council on Alcohol, Drugs and Traffic Society (ICADTS), Abstracts. 2010. 169–169. Print.
@inproceedings{1166607,
  abstract     = {Objectives: DRUID (Driving Under the Inflluence of Drugs, Alcohol and Medicines) is a research project funded by the European Commission in which oral fluid is analysed by eleven laboratories. A common collection and analysis methodology has been used: collection with Statsure Saliva Sampler and LC-MS/MS or GC-MS analysis of 22 substances. Four rounds of proficiency testing (PT) were organized between March 2008 and September 2009.
Methods: Qualitative results were evaluated using sensitivity and specificity. Quantitative results were evaluated using z-scores and the standard deviation of Horwitz.
Results: Specificity was above 99\% for all analytes; sensitivity per analyte varied between 81.7 and 100\%, 20 out of 22 analytes had a sensitivity {\textrangle}90\%. The percentage of satisfactory z-scores increased from 79.4\% to 89.2\% over the test period. This trend was seen for all drugs, except zopiclone. Results were discussed with participating laboratories and problems were addressed. 
Conclusions: Because of these corrective actions, DRUID laboratories have a lower variation in results than previously published PT schemes in oral fluid.},
  author       = {Verstraete, Alain and Pil, Kristof and Esposito, Francis},
  booktitle    = {Tri-annual meeting of the International Council on Alcohol, Drugs and Traffic Society (ICADTS), Abstracts},
  language     = {eng},
  location     = {Oslo, Norway},
  pages        = {169--169},
  title        = {External quality assessment of multi-analyte chromatographic methods in oral fluid},
  year         = {2010},
}