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The effect of caspase-inhibitors on radiation induced apoptosis in human peripheral blood lymphocytes: an electron microscopic approach

Maria Cornelissen (UGent) , Anne Vral (UGent) , Hubert Thierens (UGent) and Leo De Ridder (UGent)
(1999) APOPTOSIS. 4(6). p.449-454
Author
Organization
Abstract
Resting lymphocytes are sensitive to radiation damage and die by apoptosis. We investigated the effect of caspase-inhibitors on radiation induced apoptosis in human peripheral blood lymphocytes. Lymphocytes were irradiated in vitro with 5 Gy Co-60-gamma-rays and cultured for 24 hours in the presence or absence of the caspase-inhibitors zVAD-fmk and zDEVD-fmk. Cell death was evaluated by electron microscopy. Irradiation in the absence of the inhibitors resulted in about 30% dead cells, almost all showing typical apoptotic morphologies. Addition of either one of the inhibitors could not rescue cells from death. Part of the dead lymphocytes (about 65%) still showed typical nuclear characteristics of apoptotic cells: sharply marginated, condensed chromatin, clumped into one sphere or into a crescent shaped mass. The remaining part of the dead cells had ultrastructural characteristics, aberrant from apoptic cells: clumping of the chromatin was less pronounced and less sharply marginated. Irregular clumps were formed. Data indicate that part of the lymphocytes go in apoptosis in a caspase-independent way. The other part shows caspase-dependent apoptosis with respect to the nuclear events.
Keywords
caspase-inhibitors, apoptosis, electron microscopy, ionizing radiation, lymphocytes, CELL-DEATH, COMMITMENT, EXECUTIONERS

Citation

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Chicago
Cornelissen, Maria, Anne Vral, Hubert Thierens, and Leo De Ridder. 1999. “The Effect of Caspase-inhibitors on Radiation Induced Apoptosis in Human Peripheral Blood Lymphocytes: An Electron Microscopic Approach.” Apoptosis 4 (6): 449–454.
APA
Cornelissen, Maria, Vral, A., Thierens, H., & De Ridder, L. (1999). The effect of caspase-inhibitors on radiation induced apoptosis in human peripheral blood lymphocytes: an electron microscopic approach. APOPTOSIS, 4(6), 449–454.
Vancouver
1.
Cornelissen M, Vral A, Thierens H, De Ridder L. The effect of caspase-inhibitors on radiation induced apoptosis in human peripheral blood lymphocytes: an electron microscopic approach. APOPTOSIS. 1999;4(6):449–54.
MLA
Cornelissen, Maria, Anne Vral, Hubert Thierens, et al. “The Effect of Caspase-inhibitors on Radiation Induced Apoptosis in Human Peripheral Blood Lymphocytes: An Electron Microscopic Approach.” APOPTOSIS 4.6 (1999): 449–454. Print.
@article{116441,
  abstract     = {Resting lymphocytes are sensitive to radiation damage and die by apoptosis. We investigated the effect of caspase-inhibitors on radiation induced apoptosis in human peripheral blood lymphocytes. Lymphocytes were irradiated in vitro with 5 Gy Co-60-gamma-rays and cultured for 24 hours in the presence or absence of the caspase-inhibitors zVAD-fmk and zDEVD-fmk. Cell death was evaluated by electron microscopy. Irradiation in the absence of the inhibitors resulted in about 30\% dead cells, almost all showing typical apoptotic morphologies. Addition of either one of the inhibitors could not rescue cells from death. Part of the dead lymphocytes (about 65\%) still showed typical nuclear characteristics of apoptotic cells: sharply marginated, condensed chromatin, clumped into one sphere or into a crescent shaped mass. The remaining part of the dead cells had ultrastructural characteristics, aberrant from apoptic cells: clumping of the chromatin was less pronounced and less sharply marginated. Irregular clumps were formed. Data indicate that part of the lymphocytes go in apoptosis in a caspase-independent way. The other part shows caspase-dependent apoptosis with respect to the nuclear events.},
  author       = {Cornelissen, Maria and Vral, Anne and Thierens, Hubert and De Ridder, Leo},
  issn         = {1360-8185},
  journal      = {APOPTOSIS},
  keyword      = {caspase-inhibitors,apoptosis,electron microscopy,ionizing radiation,lymphocytes,CELL-DEATH,COMMITMENT,EXECUTIONERS},
  language     = {eng},
  number       = {6},
  pages        = {449--454},
  title        = {The effect of caspase-inhibitors on radiation induced apoptosis in human peripheral blood lymphocytes: an electron microscopic approach},
  url          = {http://dx.doi.org/10.1023/A:1009652426259},
  volume       = {4},
  year         = {1999},
}

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